Lactococcus phage LW81

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Audreyjarvisvirus; Lactococcus virus LW81

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 178 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JN25|A0A1W6JN25_9CAUD Uncharacterized protein OS=Lactococcus phage LW81 OX=1965482 GN=LW81_038 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 4.21NTKK5 pKa = 10.3EE6 pKa = 3.96FNQLTIDD13 pKa = 3.7VTEE16 pKa = 4.73LGLFNGLYY24 pKa = 7.9EE25 pKa = 4.71TIWLNDD31 pKa = 3.16NMDD34 pKa = 3.35IDD36 pKa = 4.49EE37 pKa = 4.31VMEE40 pKa = 4.38LADD43 pKa = 3.81MLEE46 pKa = 4.11VDD48 pKa = 4.14WNDD51 pKa = 2.61IDD53 pKa = 5.45VSIDD57 pKa = 3.28CNEE60 pKa = 3.8YY61 pKa = 10.87LEE63 pKa = 5.67AIGEE67 pKa = 4.42LYY69 pKa = 10.98CEE71 pKa = 4.35MFCNEE76 pKa = 4.97LDD78 pKa = 3.77SKK80 pKa = 11.57GLFRR84 pKa = 11.84VDD86 pKa = 3.0SLYY89 pKa = 11.01SPRR92 pKa = 11.84WYY94 pKa = 10.76NYY96 pKa = 8.09DD97 pKa = 3.36TDD99 pKa = 5.35HH100 pKa = 7.03ITITWDD106 pKa = 3.44SEE108 pKa = 4.08LSLEE112 pKa = 5.07DD113 pKa = 3.66MEE115 pKa = 5.68SKK117 pKa = 10.69LKK119 pKa = 9.86EE120 pKa = 3.82LCYY123 pKa = 10.53DD124 pKa = 4.54IINAYY129 pKa = 9.97DD130 pKa = 3.29WTIEE134 pKa = 3.93EE135 pKa = 5.07SIWDD139 pKa = 3.59NKK141 pKa = 9.24GRR143 pKa = 11.84EE144 pKa = 4.3LYY146 pKa = 11.17SNMVRR151 pKa = 11.84YY152 pKa = 7.88TYY154 pKa = 10.75KK155 pKa = 10.82NKK157 pKa = 9.83PLWFGMDD164 pKa = 3.05SKK166 pKa = 11.39DD167 pKa = 3.22IAEE170 pKa = 4.4VKK172 pKa = 10.43GG173 pKa = 3.47

Molecular weight: 20.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JN47|A0A1W6JN47_9CAUD Uncharacterized protein OS=Lactococcus phage LW81 OX=1965482 GN=LW81_084 PE=4 SV=1
MM1 pKa = 7.08FKK3 pKa = 10.67KK4 pKa = 10.45RR5 pKa = 11.84ITRR8 pKa = 11.84LIILIVSYY16 pKa = 10.71LFLVGVSWIFPTIHH30 pKa = 5.22MTNNIFKK37 pKa = 10.42NIAILLVGQPILNMFIAGCIAMVSIFLALLAYY69 pKa = 10.03AIIYY73 pKa = 10.12AVVKK77 pKa = 9.83WVMKK81 pKa = 10.32GDD83 pKa = 3.59KK84 pKa = 10.4

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

178

0

178

36357

29

3213

204.3

23.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.896 ± 0.338

0.624 ± 0.073

6.909 ± 0.176

8.067 ± 0.294

4.481 ± 0.202

5.584 ± 0.236

1.436 ± 0.108

7.748 ± 0.146

9.407 ± 0.202

8.271 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.616 ± 0.096

6.582 ± 0.177

2.181 ± 0.091

3.331 ± 0.261

3.543 ± 0.136

6.576 ± 0.271

5.903 ± 0.19

6.227 ± 0.165

1.073 ± 0.079

4.547 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski