Raoultella phage RP180

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; Raoultella virus RP180

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DY72|A0A4D6DY72_9CAUD DUF551 domain-containing protein OS=Raoultella phage RP180 OX=2565500 GN=RP180_48 PE=4 SV=1
MM1 pKa = 7.22MEE3 pKa = 4.34EE4 pKa = 4.61LICVKK9 pKa = 10.67SKK11 pKa = 10.95LSEE14 pKa = 4.16FKK16 pKa = 10.73PGGLYY21 pKa = 10.43SSYY24 pKa = 9.9STSDD28 pKa = 3.01YY29 pKa = 11.1DD30 pKa = 4.02YY31 pKa = 11.43VIDD34 pKa = 3.77EE35 pKa = 4.78TGGEE39 pKa = 4.13WVVDD43 pKa = 3.38QFGDD47 pKa = 3.8DD48 pKa = 3.27EE49 pKa = 5.26FGIFDD54 pKa = 4.46GGLKK58 pKa = 10.58ASFTTASNHH67 pKa = 6.23DD68 pKa = 3.42ARR70 pKa = 11.84ADD72 pKa = 3.68DD73 pKa = 4.21SEE75 pKa = 4.7GGCRR79 pKa = 11.84GG80 pKa = 3.27

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DYI0|A0A4D6DYI0_9CAUD Uncharacterized protein OS=Raoultella phage RP180 OX=2565500 GN=RP180_53 PE=4 SV=1
MM1 pKa = 7.21LRR3 pKa = 11.84SVAKK7 pKa = 9.64TIFISFSPHH16 pKa = 4.69VLRR19 pKa = 11.84LPAYY23 pKa = 7.99GALRR27 pKa = 11.84GSSLPVRR34 pKa = 11.84RR35 pKa = 11.84NQRR38 pKa = 11.84RR39 pKa = 11.84VSGGAAVEE47 pKa = 4.31LLTSSLYY54 pKa = 10.71NPCAYY59 pKa = 10.4FDD61 pKa = 4.09GLYY64 pKa = 10.38FSPDD68 pKa = 2.93VLALGFLLFTAGKK81 pKa = 8.52PRR83 pKa = 11.84VHH85 pKa = 7.13GIIEE89 pKa = 4.33TTFTT93 pKa = 3.63

Molecular weight: 10.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13583

45

814

212.2

23.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.681 ± 0.475

1.178 ± 0.171

6.111 ± 0.21

6.766 ± 0.351

3.872 ± 0.158

7.568 ± 0.303

1.833 ± 0.186

5.338 ± 0.152

5.86 ± 0.312

7.738 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.135

4.542 ± 0.241

4.005 ± 0.184

3.652 ± 0.311

5.639 ± 0.277

6.015 ± 0.343

6.25 ± 0.321

6.817 ± 0.28

1.355 ± 0.146

3.364 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski