Gordonia sp. NB4-1Y

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Gordoniaceae; Gordonia; unclassified Gordonia

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N0UMM5|A0A0N0UMM5_9ACTN Uncharacterized protein OS=Gordonia sp. NB4-1Y OX=1241906 GN=ISGA_11360 PE=4 SV=1
MM1 pKa = 7.17TNQWTGGQQTAGIDD15 pKa = 3.58SSTTTSASGTGSVAVGGDD33 pKa = 3.2ASGPIATTGGVAGDD47 pKa = 4.0GNSVGNSYY55 pKa = 10.84DD56 pKa = 3.88DD57 pKa = 3.47HH58 pKa = 6.31STWVDD63 pKa = 3.42QSVHH67 pKa = 5.81GNNSGNTQNTHH78 pKa = 6.92IEE80 pKa = 4.26DD81 pKa = 3.51NSTNDD86 pKa = 2.77SHH88 pKa = 6.87NQLNVHH94 pKa = 6.78AGGGDD99 pKa = 3.5GGDD102 pKa = 3.88GGDD105 pKa = 3.42GGGWGFGGAGDD116 pKa = 4.41GGDD119 pKa = 3.33GGGNVDD125 pKa = 5.4LSGIDD130 pKa = 3.2LGYY133 pKa = 9.05TRR135 pKa = 11.84GGITSDD141 pKa = 3.12NSNHH145 pKa = 4.6YY146 pKa = 9.53TEE148 pKa = 4.76THH150 pKa = 6.89DD151 pKa = 3.88SHH153 pKa = 7.66DD154 pKa = 3.77ISDD157 pKa = 3.85SFNDD161 pKa = 3.1EE162 pKa = 3.86RR163 pKa = 11.84SYY165 pKa = 11.73DD166 pKa = 3.66SHH168 pKa = 8.4DD169 pKa = 4.44DD170 pKa = 3.42NSTNDD175 pKa = 3.31SFNDD179 pKa = 3.58NSQHH183 pKa = 4.51THH185 pKa = 5.7SEE187 pKa = 4.19SGFDD191 pKa = 4.28PGDD194 pKa = 3.6LLGGVADD201 pKa = 4.15AAGGVAGAYY210 pKa = 10.2GDD212 pKa = 3.82VAGGIAGGIAGGVADD227 pKa = 4.67AAGGIADD234 pKa = 3.84VAGDD238 pKa = 3.81AVDD241 pKa = 5.21AIGDD245 pKa = 3.78ALGDD249 pKa = 3.59IFF251 pKa = 5.98

Molecular weight: 24.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M7A5B1|M7A5B1_9ACTN Oxidoreductase OS=Gordonia sp. NB4-1Y OX=1241906 GN=ISGA_4823 PE=4 SV=2
MM1 pKa = 7.59KK2 pKa = 10.19VRR4 pKa = 11.84SSLRR8 pKa = 11.84SLKK11 pKa = 9.98KK12 pKa = 9.94QPGSQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84VFVINKK30 pKa = 8.12GNPRR34 pKa = 11.84FKK36 pKa = 10.78ARR38 pKa = 11.84QGG40 pKa = 3.28

Molecular weight: 4.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4263

0

4263

1343207

32

4939

315.1

33.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.771 ± 0.05

0.752 ± 0.009

6.742 ± 0.029

5.2 ± 0.035

2.991 ± 0.022

9.039 ± 0.034

2.261 ± 0.017

4.633 ± 0.026

2.055 ± 0.025

9.455 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.013 ± 0.015

2.06 ± 0.018

5.603 ± 0.029

2.72 ± 0.018

7.265 ± 0.033

5.515 ± 0.023

6.559 ± 0.026

8.878 ± 0.036

1.454 ± 0.015

2.035 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski