Staphylococcus phage DW2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Phietavirus; unclassified Phietavirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059T621|A0A059T621_9CAUD Uncharacterized protein OS=Staphylococcus phage DW2 OX=1464127 GN=DW2_26 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.45VEE20 pKa = 3.82YY21 pKa = 10.92QHH23 pKa = 6.96FDD25 pKa = 3.41DD26 pKa = 5.2VDD28 pKa = 3.71NEE30 pKa = 4.24KK31 pKa = 9.27EE32 pKa = 4.15TLADD36 pKa = 3.54YY37 pKa = 10.81LYY39 pKa = 11.05NNPDD43 pKa = 3.26EE44 pKa = 4.53LLEE47 pKa = 3.95YY48 pKa = 11.05DD49 pKa = 3.49NLKK52 pKa = 9.69IRR54 pKa = 11.84NVNVEE59 pKa = 3.93VEE61 pKa = 4.1

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059T663|A0A059T663_9CAUD Single strand DNA binding protein OS=Staphylococcus phage DW2 OX=1464127 GN=DW2_14 PE=3 SV=1
MM1 pKa = 7.39RR2 pKa = 11.84QQVTEE7 pKa = 4.0KK8 pKa = 10.55YY9 pKa = 9.95LRR11 pKa = 11.84NKK13 pKa = 9.68IQVKK17 pKa = 10.25YY18 pKa = 8.3KK19 pKa = 9.01TKK21 pKa = 10.74NGGSQDD27 pKa = 3.28VLRR30 pKa = 11.84NRR32 pKa = 11.84RR33 pKa = 11.84CMSEE37 pKa = 4.03GNN39 pKa = 3.48

Molecular weight: 4.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12595

37

1047

203.1

23.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.875 ± 0.502

0.476 ± 0.085

6.376 ± 0.316

7.773 ± 0.576

4.24 ± 0.173

6.026 ± 0.314

1.564 ± 0.138

6.987 ± 0.271

8.964 ± 0.39

7.566 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.58 ± 0.2

6.566 ± 0.328

2.715 ± 0.22

4.248 ± 0.278

4.287 ± 0.29

5.852 ± 0.264

6.05 ± 0.311

6.368 ± 0.272

1.191 ± 0.164

4.295 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski