Eremococcus coleocola ACS-139-V-Col8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Aerococcaceae; Eremococcus; Eremococcus coleocola

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1720 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E4KQN8|E4KQN8_9LACT Oxaloacetate decarboxylase gamma chain OS=Eremococcus coleocola ACS-139-V-Col8 OX=908337 GN=HMPREF9257_0597 PE=3 SV=1
MM1 pKa = 7.23KK2 pKa = 10.42NKK4 pKa = 9.93YY5 pKa = 10.1SKK7 pKa = 10.64LSKK10 pKa = 9.18TALVLMSSLVLQAPLALAQEE30 pKa = 5.2AYY32 pKa = 9.06TVQQGDD38 pKa = 3.78TLYY41 pKa = 11.31DD42 pKa = 3.82LAQTIGASVDD52 pKa = 4.12EE53 pKa = 4.12LMAWNGLTSDD63 pKa = 4.11YY64 pKa = 11.11LDD66 pKa = 3.9VGDD69 pKa = 5.05ILYY72 pKa = 9.25LAPAAPSAEE81 pKa = 4.32STPEE85 pKa = 4.03TTSPAYY91 pKa = 10.85SNGPGYY97 pKa = 8.8HH98 pKa = 5.38TVSAGEE104 pKa = 4.05NLWSIAYY111 pKa = 10.16YY112 pKa = 10.75YY113 pKa = 8.34GTTMQAIVDD122 pKa = 4.04ANGLSSDD129 pKa = 3.89YY130 pKa = 11.18LDD132 pKa = 5.14VGDD135 pKa = 4.35QLYY138 pKa = 10.39IPAVNDD144 pKa = 3.6AYY146 pKa = 10.83GPGEE150 pKa = 3.84AVSYY154 pKa = 10.28YY155 pKa = 10.71GQEE158 pKa = 3.66QSTNSTPADD167 pKa = 3.6YY168 pKa = 10.7SSSVTPDD175 pKa = 3.0STVKK179 pKa = 10.76DD180 pKa = 3.72GAQIHH185 pKa = 5.35TVVAGDD191 pKa = 3.77NLYY194 pKa = 10.79DD195 pKa = 3.3IAIAYY200 pKa = 7.64GINLNDD206 pKa = 4.0LYY208 pKa = 10.35TWNGLSSDD216 pKa = 3.99YY217 pKa = 11.4LNVGDD222 pKa = 4.19QLIVGLAGPVEE233 pKa = 4.63DD234 pKa = 3.98NQEE237 pKa = 3.9VTVTDD242 pKa = 4.09DD243 pKa = 2.94QGQSKK248 pKa = 10.62KK249 pKa = 9.88IDD251 pKa = 3.45LSKK254 pKa = 10.51IPEE257 pKa = 4.02KK258 pKa = 10.49FRR260 pKa = 11.84PKK262 pKa = 9.96TYY264 pKa = 9.98TVVAGDD270 pKa = 4.26NVWKK274 pKa = 10.3IAQKK278 pKa = 10.72NKK280 pKa = 9.75VSAEE284 pKa = 4.18SIRR287 pKa = 11.84LWNDD291 pKa = 2.84LAEE294 pKa = 4.6DD295 pKa = 3.36QGLTVGDD302 pKa = 3.54QVYY305 pKa = 10.17VSNPALVPEE314 pKa = 3.87IHH316 pKa = 6.37EE317 pKa = 4.36VVEE320 pKa = 4.64GEE322 pKa = 4.17DD323 pKa = 3.57LASIGADD330 pKa = 3.33YY331 pKa = 10.73QVAEE335 pKa = 5.0DD336 pKa = 6.28DD337 pKa = 3.97LLAWNDD343 pKa = 2.83ISKK346 pKa = 11.27AEE348 pKa = 4.61DD349 pKa = 3.24IKK351 pKa = 11.55VGDD354 pKa = 4.26QIAVSNPKK362 pKa = 9.58PEE364 pKa = 3.88KK365 pKa = 9.99HH366 pKa = 4.97QVQAGEE372 pKa = 4.14TLEE375 pKa = 5.92DD376 pKa = 3.07IANTYY381 pKa = 9.82GVTVDD386 pKa = 6.36DD387 pKa = 4.45LRR389 pKa = 11.84TWNNLPANALIVNGEE404 pKa = 4.7LIVADD409 pKa = 4.03PQAGNSAEE417 pKa = 3.96

Molecular weight: 44.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E4KR82|E4KR82_9LACT Uncharacterized protein OS=Eremococcus coleocola ACS-139-V-Col8 OX=908337 GN=HMPREF9257_0381 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 9.2KK9 pKa = 7.96RR10 pKa = 11.84HH11 pKa = 4.8RR12 pKa = 11.84QKK14 pKa = 10.52VHH16 pKa = 5.94GFRR19 pKa = 11.84QRR21 pKa = 11.84MKK23 pKa = 8.52TKK25 pKa = 9.67NGRR28 pKa = 11.84RR29 pKa = 11.84VLRR32 pKa = 11.84KK33 pKa = 9.21RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.45GRR39 pKa = 11.84KK40 pKa = 8.5SLAVV44 pKa = 3.3

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1720

0

1720

512594

37

1721

298.0

33.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.933 ± 0.061

0.532 ± 0.015

6.217 ± 0.049

6.485 ± 0.061

4.285 ± 0.041

6.526 ± 0.055

1.986 ± 0.029

7.492 ± 0.05

6.05 ± 0.049

10.249 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.028

4.505 ± 0.043

3.517 ± 0.036

5.237 ± 0.063

3.849 ± 0.045

5.947 ± 0.04

5.058 ± 0.04

6.735 ± 0.052

0.882 ± 0.021

3.847 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski