Lake Sarah-associated circular virus-46

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GA04|A0A126GA04_9VIRU Coat protein OS=Lake Sarah-associated circular virus-46 OX=1685775 PE=4 SV=1
MM1 pKa = 7.15AQNRR5 pKa = 11.84HH6 pKa = 4.44WQITDD11 pKa = 3.49YY12 pKa = 10.76EE13 pKa = 4.63VSDD16 pKa = 4.08EE17 pKa = 4.61PYY19 pKa = 10.7VIPQYY24 pKa = 11.44GDD26 pKa = 3.26YY27 pKa = 10.75TVGQVEE33 pKa = 4.51RR34 pKa = 11.84CPDD37 pKa = 3.15TGQLHH42 pKa = 5.35WQLYY46 pKa = 9.25CYY48 pKa = 9.73TRR50 pKa = 11.84TKK52 pKa = 10.94LSVKK56 pKa = 9.84AFKK59 pKa = 10.28KK60 pKa = 10.17RR61 pKa = 11.84YY62 pKa = 5.3PTVHH66 pKa = 6.75FEE68 pKa = 3.73IARR71 pKa = 11.84NPEE74 pKa = 3.56ALRR77 pKa = 11.84NYY79 pKa = 7.84GQKK82 pKa = 10.57EE83 pKa = 4.13EE84 pKa = 4.26TRR86 pKa = 11.84VAGPFTWGEE95 pKa = 3.71APEE98 pKa = 4.1QGKK101 pKa = 7.82RR102 pKa = 11.84TDD104 pKa = 3.19LHH106 pKa = 5.95EE107 pKa = 6.23AIATWVTHH115 pKa = 6.02GKK117 pKa = 10.05RR118 pKa = 11.84KK119 pKa = 9.72ACEE122 pKa = 3.85DD123 pKa = 3.73HH124 pKa = 6.45PHH126 pKa = 5.36VVAKK130 pKa = 9.39FYY132 pKa = 11.05KK133 pKa = 10.14GIQFVAEE140 pKa = 4.25ALRR143 pKa = 11.84PRR145 pKa = 11.84YY146 pKa = 9.35VPPVLEE152 pKa = 4.6LRR154 pKa = 11.84DD155 pKa = 3.84HH156 pKa = 6.59QRR158 pKa = 11.84TLLDD162 pKa = 3.52SFLSHH167 pKa = 6.59NNPRR171 pKa = 11.84HH172 pKa = 5.03VYY174 pKa = 9.91YY175 pKa = 10.68LYY177 pKa = 10.51DD178 pKa = 3.48EE179 pKa = 5.25CGGSGKK185 pKa = 10.36SRR187 pKa = 11.84FAAHH191 pKa = 6.76IRR193 pKa = 11.84DD194 pKa = 3.61HH195 pKa = 6.74HH196 pKa = 7.2GGIILDD202 pKa = 4.02EE203 pKa = 4.45GNIADD208 pKa = 3.89WALIYY213 pKa = 10.82DD214 pKa = 4.16GEE216 pKa = 4.55PYY218 pKa = 10.78VIFDD222 pKa = 4.4LARR225 pKa = 11.84AQEE228 pKa = 4.18KK229 pKa = 9.91GVPLIVPRR237 pKa = 11.84FIEE240 pKa = 4.11YY241 pKa = 10.45LSNGSITSPKK251 pKa = 9.9YY252 pKa = 8.62EE253 pKa = 4.18SKK255 pKa = 11.19VKK257 pKa = 10.3IFKK260 pKa = 10.32QPQCMIMSNTRR271 pKa = 11.84CDD273 pKa = 3.27ITTLSADD280 pKa = 3.23RR281 pKa = 11.84WIHH284 pKa = 5.9VDD286 pKa = 3.23WSNHH290 pKa = 3.9GQGSQVPSAFQAA302 pKa = 3.48

Molecular weight: 34.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GA04|A0A126GA04_9VIRU Coat protein OS=Lake Sarah-associated circular virus-46 OX=1685775 PE=4 SV=1
MM1 pKa = 7.63AKK3 pKa = 9.42GAKK6 pKa = 8.46YY7 pKa = 10.04RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84SKK12 pKa = 10.86PKK14 pKa = 7.66TAKK17 pKa = 9.74FKK19 pKa = 10.8KK20 pKa = 9.81SRR22 pKa = 11.84QTTKK26 pKa = 9.96PSPAFKK32 pKa = 10.83VKK34 pKa = 9.71VKK36 pKa = 10.71KK37 pKa = 10.69VMRR40 pKa = 11.84SQEE43 pKa = 4.11EE44 pKa = 4.04TKK46 pKa = 9.68MAYY49 pKa = 9.67AQIVSKK55 pKa = 9.07TLHH58 pKa = 6.19NSPINTQADD67 pKa = 3.89WYY69 pKa = 10.44RR70 pKa = 11.84VYY72 pKa = 10.46PQLGQTTDD80 pKa = 2.83ANARR84 pKa = 11.84IGNEE88 pKa = 3.99VYY90 pKa = 7.96PTRR93 pKa = 11.84LVLDD97 pKa = 4.13LDD99 pKa = 3.63ISLRR103 pKa = 11.84AADD106 pKa = 4.63DD107 pKa = 3.79RR108 pKa = 11.84GVDD111 pKa = 3.53LTVVVLIAKK120 pKa = 9.73SIAIRR125 pKa = 11.84GTPQGSVNGTFLPFAEE141 pKa = 4.24YY142 pKa = 10.24FKK144 pKa = 10.63TDD146 pKa = 3.46EE147 pKa = 4.28ATVTAFDD154 pKa = 4.17GSVNTVDD161 pKa = 5.33FPWNNEE167 pKa = 3.77QVKK170 pKa = 10.64GIARR174 pKa = 11.84KK175 pKa = 7.36TFRR178 pKa = 11.84LSKK181 pKa = 10.86GGGLNTGQNNHH192 pKa = 6.88PSQPNDD198 pKa = 3.26GFSDD202 pKa = 3.79SPYY205 pKa = 11.36CMLKK209 pKa = 10.31KK210 pKa = 10.44LRR212 pKa = 11.84FNLPVPKK219 pKa = 9.64ILKK222 pKa = 9.39YY223 pKa = 10.92DD224 pKa = 4.79PISVGSGSAFPNNDD238 pKa = 2.68HH239 pKa = 6.57YY240 pKa = 11.45YY241 pKa = 9.08YY242 pKa = 11.21AVGYY246 pKa = 9.19YY247 pKa = 10.28YY248 pKa = 11.31NNLDD252 pKa = 3.44VAPDD256 pKa = 3.58VLIGQSLIVNARR268 pKa = 11.84SRR270 pKa = 11.84LYY272 pKa = 11.09YY273 pKa = 10.34KK274 pKa = 10.73DD275 pKa = 3.2GG276 pKa = 3.75

Molecular weight: 30.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

578

276

302

289.0

32.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.266 ± 0.225

1.211 ± 0.557

5.882 ± 0.182

4.325 ± 1.411

3.806 ± 0.118

6.747 ± 0.09

3.114 ± 1.33

5.19 ± 0.553

6.92 ± 1.165

6.401 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.211 ± 0.156

4.671 ± 1.214

6.055 ± 0.068

4.671 ± 0.45

6.228 ± 0.283

5.882 ± 0.657

5.882 ± 0.419

7.266 ± 0.462

1.557 ± 0.546

5.709 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski