Mycobacterium phage GS4E

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5XCA8|A0A2Z5XCA8_9CAUD Uncharacterized protein OS=Mycobacterium phage GS4E OX=2072004 PE=4 SV=1
MM1 pKa = 7.44FGDD4 pKa = 5.8RR5 pKa = 11.84YY6 pKa = 10.31IDD8 pKa = 3.45WDD10 pKa = 3.36GHH12 pKa = 4.31EE13 pKa = 4.57QYY15 pKa = 10.88TLFDD19 pKa = 3.84LEE21 pKa = 5.01GIHH24 pKa = 7.51DD25 pKa = 4.32YY26 pKa = 11.04VHH28 pKa = 6.76EE29 pKa = 5.15GDD31 pKa = 5.19DD32 pKa = 3.82EE33 pKa = 4.27EE34 pKa = 4.55

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5XCE3|A0A2Z5XCE3_9CAUD Uncharacterized protein OS=Mycobacterium phage GS4E OX=2072004 PE=4 SV=1
MM1 pKa = 7.63TFTKK5 pKa = 10.5LPYY8 pKa = 8.63LHH10 pKa = 7.01KK11 pKa = 10.35NARR14 pKa = 11.84SRR16 pKa = 11.84QITSRR21 pKa = 11.84EE22 pKa = 3.78VRR24 pKa = 11.84EE25 pKa = 3.98VFAAEE30 pKa = 3.86VTRR33 pKa = 11.84NLDD36 pKa = 3.07RR37 pKa = 11.84RR38 pKa = 11.84LDD40 pKa = 3.39KK41 pKa = 11.2DD42 pKa = 3.37EE43 pKa = 3.77YY44 pKa = 10.68LRR46 pKa = 11.84RR47 pKa = 11.84VMPP50 pKa = 4.78

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

15169

34

845

172.4

19.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.856 ± 0.413

0.93 ± 0.147

6.388 ± 0.238

7.12 ± 0.43

3.336 ± 0.157

8.016 ± 0.385

1.991 ± 0.19

5.149 ± 0.18

4.845 ± 0.217

8.412 ± 0.382

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.159

3.349 ± 0.208

5.531 ± 0.272

3.784 ± 0.234

6.072 ± 0.393

5.142 ± 0.223

6.052 ± 0.344

6.863 ± 0.221

1.806 ± 0.146

2.861 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski