Microbacterium phage Percival

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4Q6J5|A0A2Z4Q6J5_9CAUD ADP-ribosyltransferase domain and MuF-like fusion protein OS=Microbacterium phage Percival OX=2201439 GN=4 PE=4 SV=1
MM1 pKa = 7.31TEE3 pKa = 5.59CIQCHH8 pKa = 5.01QLAVDD13 pKa = 3.59PADD16 pKa = 3.73PPRR19 pKa = 11.84YY20 pKa = 9.38DD21 pKa = 3.62VLAEE25 pKa = 4.29TPTGPYY31 pKa = 9.73CAEE34 pKa = 3.98CWEE37 pKa = 4.4VTVTARR43 pKa = 11.84EE44 pKa = 3.87TDD46 pKa = 3.54TPP48 pKa = 3.94

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q6N2|A0A2Z4Q6N2_9CAUD Uncharacterized protein OS=Microbacterium phage Percival OX=2201439 GN=45 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84KK3 pKa = 9.54APSARR8 pKa = 11.84IVLYY12 pKa = 9.34MSNIRR17 pKa = 11.84YY18 pKa = 6.98TAAQRR23 pKa = 11.84LALAVKK29 pKa = 10.16RR30 pKa = 11.84DD31 pKa = 3.57AALVAQRR38 pKa = 11.84ATMSEE43 pKa = 3.52ADD45 pKa = 3.25IAEE48 pKa = 4.69VIGSAPGEE56 pKa = 4.07AVTLSSGRR64 pKa = 11.84VIAWADD70 pKa = 3.25FVPAYY75 pKa = 10.32RR76 pKa = 11.84AGDD79 pKa = 3.47VLNAHH84 pKa = 7.11RR85 pKa = 11.84GIIEE89 pKa = 3.88RR90 pKa = 11.84AGALRR95 pKa = 3.89

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14618

27

973

197.5

21.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.072 ± 0.507

0.493 ± 0.089

6.348 ± 0.312

5.986 ± 0.293

2.955 ± 0.17

7.983 ± 0.306

2.196 ± 0.203

4.241 ± 0.28

3.256 ± 0.275

8.941 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.847 ± 0.115

2.408 ± 0.183

5.452 ± 0.284

2.928 ± 0.173

7.368 ± 0.47

5.486 ± 0.276

6.998 ± 0.373

6.861 ± 0.255

1.724 ± 0.159

2.456 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski