Lolium perenne-associated virus

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses

Average proteome isoelectric point is 8.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345BKA8|A0A345BKA8_9VIRU Replication-associated protein OS=Lolium perenne-associated virus OX=2282644 PE=3 SV=1
MM1 pKa = 7.8SFRR4 pKa = 11.84LQSKK8 pKa = 9.94ILLLTYY14 pKa = 10.34SQADD18 pKa = 4.01AIDD21 pKa = 3.55HH22 pKa = 5.82EE23 pKa = 4.78RR24 pKa = 11.84LFKK27 pKa = 10.6FLSGLKK33 pKa = 9.09QPIRR37 pKa = 11.84IRR39 pKa = 11.84VGKK42 pKa = 8.18EE43 pKa = 3.04AHH45 pKa = 6.34MDD47 pKa = 3.35GHH49 pKa = 5.28THH51 pKa = 4.32YY52 pKa = 10.3HH53 pKa = 7.35AYY55 pKa = 10.46AEE57 pKa = 4.25WEE59 pKa = 4.38KK60 pKa = 11.03KK61 pKa = 10.6YY62 pKa = 9.18EE63 pKa = 4.25TTDD66 pKa = 2.89ARR68 pKa = 11.84HH69 pKa = 6.12FDD71 pKa = 3.86FEE73 pKa = 5.21SIHH76 pKa = 6.25PNIVSRR82 pKa = 11.84VRR84 pKa = 11.84DD85 pKa = 3.69PLSAWNYY92 pKa = 8.56CGKK95 pKa = 10.43DD96 pKa = 3.46GVAIDD101 pKa = 4.43YY102 pKa = 9.85GEE104 pKa = 4.62CPQPRR109 pKa = 11.84AINKK113 pKa = 8.4RR114 pKa = 11.84KK115 pKa = 10.21QMMDD119 pKa = 2.85AFDD122 pKa = 3.72ASSRR126 pKa = 11.84QEE128 pKa = 3.78FMEE131 pKa = 4.5MIKK134 pKa = 10.7NADD137 pKa = 3.34PGRR140 pKa = 11.84YY141 pKa = 8.65IFQHH145 pKa = 5.53QNLEE149 pKa = 4.13YY150 pKa = 10.21FANKK154 pKa = 8.88QFAVCIPQYY163 pKa = 11.24VSDD166 pKa = 5.08PDD168 pKa = 4.51LIFNIPDD175 pKa = 3.68EE176 pKa = 4.37LTTWLSQRR184 pKa = 11.84HH185 pKa = 4.72EE186 pKa = 4.04ADD188 pKa = 3.56RR189 pKa = 11.84PKK191 pKa = 11.04SLILTGPSRR200 pKa = 11.84TGKK203 pKa = 8.23TKK205 pKa = 9.29WARR208 pKa = 11.84SLGRR212 pKa = 11.84HH213 pKa = 6.08IYY215 pKa = 9.53WNGAFDD221 pKa = 6.19LSIFDD226 pKa = 4.8NDD228 pKa = 3.04AQYY231 pKa = 11.39AIFDD235 pKa = 5.0DD236 pKa = 4.82FPDD239 pKa = 3.04WDD241 pKa = 3.84RR242 pKa = 11.84FVQYY246 pKa = 10.15KK247 pKa = 8.91QWLGAQQHH255 pKa = 5.3FTATDD260 pKa = 3.1KK261 pKa = 11.43YY262 pKa = 10.17MKK264 pKa = 9.23KK265 pKa = 7.79TQIKK269 pKa = 8.2WGKK272 pKa = 8.38PCIILSNQNPLFKK285 pKa = 10.42DD286 pKa = 3.54YY287 pKa = 11.45LWIKK291 pKa = 10.16EE292 pKa = 4.01NTIEE296 pKa = 5.2CEE298 pKa = 4.1IKK300 pKa = 9.78TKK302 pKa = 10.76LYY304 pKa = 10.98

Molecular weight: 35.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345BKA8|A0A345BKA8_9VIRU Replication-associated protein OS=Lolium perenne-associated virus OX=2282644 PE=3 SV=1
MM1 pKa = 7.37VRR3 pKa = 11.84GTRR6 pKa = 11.84KK7 pKa = 9.5RR8 pKa = 11.84KK9 pKa = 9.56FKK11 pKa = 10.28GGHH14 pKa = 4.58IAIPRR19 pKa = 11.84KK20 pKa = 9.43KK21 pKa = 10.5VKK23 pKa = 9.61TGFRR27 pKa = 11.84NIKK30 pKa = 9.67KK31 pKa = 10.1KK32 pKa = 10.03VGGRR36 pKa = 11.84RR37 pKa = 11.84GSSSGVVMQSGMTSKK52 pKa = 9.8TKK54 pKa = 10.73LLVAGHH60 pKa = 6.74KK61 pKa = 10.84LNTKK65 pKa = 7.69VLKK68 pKa = 10.62LLSNPIRR75 pKa = 11.84VIVEE79 pKa = 3.99GNKK82 pKa = 10.13SVGANGMNYY91 pKa = 10.63AKK93 pKa = 10.68GEE95 pKa = 4.22YY96 pKa = 9.99LYY98 pKa = 10.27MDD100 pKa = 4.76ASQFDD105 pKa = 3.48IMAQLGFRR113 pKa = 11.84QRR115 pKa = 11.84AVSASGSLPAAIPGRR130 pKa = 11.84TFKK133 pKa = 10.93AWIQSFYY140 pKa = 11.52VEE142 pKa = 3.7QKK144 pKa = 9.66YY145 pKa = 11.11INMSNMAVKK154 pKa = 10.5LIIYY158 pKa = 8.16DD159 pKa = 3.74VKK161 pKa = 11.04VKK163 pKa = 10.46RR164 pKa = 11.84DD165 pKa = 3.49TSSGPFTCVQNDD177 pKa = 3.58VAILNQNRR185 pKa = 11.84DD186 pKa = 3.35TTITNDD192 pKa = 3.16AALATLTPGYY202 pKa = 10.78SPMDD206 pKa = 3.46SLQFRR211 pKa = 11.84THH213 pKa = 4.48WKK215 pKa = 8.1IVRR218 pKa = 11.84RR219 pKa = 11.84VQVHH223 pKa = 6.2LDD225 pKa = 2.93AGGVHH230 pKa = 5.77EE231 pKa = 6.11HH232 pKa = 7.39IIQANYY238 pKa = 10.41NKK240 pKa = 10.07IISRR244 pKa = 11.84NVSVEE249 pKa = 3.67DD250 pKa = 4.03NYY252 pKa = 11.59LKK254 pKa = 10.64GYY256 pKa = 10.07SFGVYY261 pKa = 9.5IEE263 pKa = 4.15VQPYY267 pKa = 9.59PIVDD271 pKa = 4.04GVTHH275 pKa = 7.4ALPSVAPPASEE286 pKa = 4.05VLMTSVGKK294 pKa = 10.28FSFRR298 pKa = 11.84EE299 pKa = 3.68MGMTPEE305 pKa = 5.07HH306 pKa = 6.98IEE308 pKa = 3.88VTNIWTLATNPTTVVDD324 pKa = 4.08EE325 pKa = 4.83SGLVTPGVTVV335 pKa = 3.59

Molecular weight: 36.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

639

304

335

319.5

36.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.729 ± 0.123

0.939 ± 0.484

5.634 ± 1.551

4.069 ± 0.593

4.538 ± 0.948

6.416 ± 1.468

2.973 ± 0.442

7.042 ± 0.359

7.668 ± 0.155

6.416 ± 0.337

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.973 ± 0.46

4.695 ± 0.513

4.538 ± 0.046

4.695 ± 0.841

5.477 ± 0.079

6.416 ± 0.791

5.947 ± 0.92

6.729 ± 3.036

1.878 ± 0.742

4.225 ± 0.486
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski