Amino acid dipepetide frequency for Nocardioides sp. GY 10113

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.191AlaAla: 22.191 ± 0.227	1.008AlaCys: 1.008 ± 0.026	9.329AlaAsp: 9.329 ± 0.097	9.097AlaGlu: 9.097 ± 0.117	3.62AlaPhe: 3.62 ± 0.058	13.815AlaGly: 13.815 ± 0.118	2.541AlaHis: 2.541 ± 0.053	4.655AlaIle: 4.655 ± 0.074	2.391AlaLys: 2.391 ± 0.061	14.183AlaLeu: 14.183 ± 0.133	2.818AlaMet: 2.818 ± 0.051	2.0AlaAsn: 2.0 ± 0.042	7.161AlaPro: 7.161 ± 0.102	3.382AlaGln: 3.382 ± 0.052	9.796AlaArg: 9.796 ± 0.118	6.469AlaSer: 6.469 ± 0.076	8.184AlaThr: 8.184 ± 0.1	12.252AlaVal: 12.252 ± 0.122	2.031AlaTrp: 2.031 ± 0.039	2.571AlaTyr: 2.571 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.932CysAla: 0.932 ± 0.029	0.09CysCys: 0.09 ± 0.009	0.457CysAsp: 0.457 ± 0.021	0.355CysGlu: 0.355 ± 0.017	0.213CysPhe: 0.213 ± 0.013	0.818CysGly: 0.818 ± 0.027	0.193CysHis: 0.193 ± 0.013	0.207CysIle: 0.207 ± 0.013	0.08CysLys: 0.08 ± 0.008	0.664CysLeu: 0.664 ± 0.023	0.095CysMet: 0.095 ± 0.008	0.119CysAsn: 0.119 ± 0.009	0.445CysPro: 0.445 ± 0.019	0.162CysGln: 0.162 ± 0.012	0.542CysArg: 0.542 ± 0.02	0.397CysSer: 0.397 ± 0.018	0.45CysThr: 0.45 ± 0.029	0.56CysVal: 0.56 ± 0.021	0.104CysTrp: 0.104 ± 0.01	0.164CysTyr: 0.164 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.665AspAla: 8.665 ± 0.09	0.367AspCys: 0.367 ± 0.019	4.157AspAsp: 4.157 ± 0.07	4.16AspGlu: 4.16 ± 0.07	1.645AspPhe: 1.645 ± 0.035	6.849AspGly: 6.849 ± 0.097	1.522AspHis: 1.522 ± 0.044	1.802AspIle: 1.802 ± 0.04	0.991AspLys: 0.991 ± 0.03	7.65AspLeu: 7.65 ± 0.088	0.764AspMet: 0.764 ± 0.025	0.901AspAsn: 0.901 ± 0.026	4.876AspPro: 4.876 ± 0.072	1.775AspGln: 1.775 ± 0.039	5.186AspArg: 5.186 ± 0.069	2.307AspSer: 2.307 ± 0.045	2.729AspThr: 2.729 ± 0.048	5.56AspVal: 5.56 ± 0.071	0.965AspTrp: 0.965 ± 0.029	1.262AspTyr: 1.262 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.716GluAla: 7.716 ± 0.097	0.325GluCys: 0.325 ± 0.015	3.134GluAsp: 3.134 ± 0.057	3.724GluGlu: 3.724 ± 0.064	1.486GluPhe: 1.486 ± 0.032	4.443GluGly: 4.443 ± 0.073	1.551GluHis: 1.551 ± 0.039	2.558GluIle: 2.558 ± 0.048	1.229GluLys: 1.229 ± 0.04	6.194GluLeu: 6.194 ± 0.074	1.002GluMet: 1.002 ± 0.031	0.931GluAsn: 0.931 ± 0.029	3.316GluPro: 3.316 ± 0.056	2.126GluGln: 2.126 ± 0.051	5.465GluArg: 5.465 ± 0.075	2.579GluSer: 2.579 ± 0.037	2.871GluThr: 2.871 ± 0.051	5.823GluVal: 5.823 ± 0.069	0.833GluTrp: 0.833 ± 0.026	1.097GluTyr: 1.097 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	3.801PheAla: 3.801 ± 0.057	0.265PheCys: 0.265 ± 0.016	2.035PheAsp: 2.035 ± 0.041	1.571PheGlu: 1.571 ± 0.037	0.892PhePhe: 0.892 ± 0.029	3.135PheGly: 3.135 ± 0.052	0.577PheHis: 0.577 ± 0.023	0.758PheIle: 0.758 ± 0.029	0.468PheLys: 0.468 ± 0.019	2.586PheLeu: 2.586 ± 0.046	0.361PheMet: 0.361 ± 0.019	0.578PheAsn: 0.578 ± 0.022	1.313PhePro: 1.313 ± 0.031	0.608PheGln: 0.608 ± 0.021	1.717PheArg: 1.717 ± 0.041	1.485PheSer: 1.485 ± 0.034	1.841PheThr: 1.841 ± 0.039	2.424PheVal: 2.424 ± 0.047	0.432PheTrp: 0.432 ± 0.023	0.612PheTyr: 0.612 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	11.832GlyAla: 11.832 ± 0.102	0.775GlyCys: 0.775 ± 0.029	5.81GlyAsp: 5.81 ± 0.105	5.507GlyGlu: 5.507 ± 0.071	2.964GlyPhe: 2.964 ± 0.046	8.723GlyGly: 8.723 ± 0.109	2.065GlyHis: 2.065 ± 0.044	3.702GlyIle: 3.702 ± 0.067	1.944GlyLys: 1.944 ± 0.044	9.586GlyLeu: 9.586 ± 0.089	1.978GlyMet: 1.978 ± 0.04	1.671GlyAsn: 1.671 ± 0.046	4.847GlyPro: 4.847 ± 0.064	2.57GlyGln: 2.57 ± 0.056	7.506GlyArg: 7.506 ± 0.085	5.506GlySer: 5.506 ± 0.064	6.013GlyThr: 6.013 ± 0.077	8.344GlyVal: 8.344 ± 0.091	1.789GlyTrp: 1.789 ± 0.042	2.276GlyTyr: 2.276 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	2.525HisAla: 2.525 ± 0.05	0.19HisCys: 0.19 ± 0.013	1.345HisAsp: 1.345 ± 0.036	1.117HisGlu: 1.117 ± 0.03	0.572HisPhe: 0.572 ± 0.022	2.16HisGly: 2.16 ± 0.044	0.658HisHis: 0.658 ± 0.028	0.484HisIle: 0.484 ± 0.022	0.265HisLys: 0.265 ± 0.014	2.392HisLeu: 2.392 ± 0.048	0.278HisMet: 0.278 ± 0.014	0.322HisAsn: 0.322 ± 0.016	1.684HisPro: 1.684 ± 0.041	0.622HisGln: 0.622 ± 0.025	1.913HisArg: 1.913 ± 0.035	0.792HisSer: 0.792 ± 0.024	0.988HisThr: 0.988 ± 0.03	1.787HisVal: 1.787 ± 0.038	0.268HisTrp: 0.268 ± 0.015	0.434HisTyr: 0.434 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.291IleAla: 5.291 ± 0.068	0.261IleCys: 0.261 ± 0.014	2.708IleAsp: 2.708 ± 0.044	2.316IleGlu: 2.316 ± 0.046	0.823IlePhe: 0.823 ± 0.027	3.92IleGly: 3.92 ± 0.071	0.63IleHis: 0.63 ± 0.021	0.938IleIle: 0.938 ± 0.026	0.734IleLys: 0.734 ± 0.024	2.627IleLeu: 2.627 ± 0.048	0.422IleMet: 0.422 ± 0.019	0.79IleAsn: 0.79 ± 0.027	1.779IlePro: 1.779 ± 0.042	0.754IleGln: 0.754 ± 0.021	2.291IleArg: 2.291 ± 0.041	1.839IleSer: 1.839 ± 0.038	2.28IleThr: 2.28 ± 0.052	3.062IleVal: 3.062 ± 0.052	0.424IleTrp: 0.424 ± 0.021	0.618IleTyr: 0.618 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	2.436LysAla: 2.436 ± 0.064	0.088LysCys: 0.088 ± 0.009	0.989LysAsp: 0.989 ± 0.031	1.015LysGlu: 1.015 ± 0.03	0.446LysPhe: 0.446 ± 0.021	1.446LysGly: 1.446 ± 0.036	0.363LysHis: 0.363 ± 0.018	0.759LysIle: 0.759 ± 0.028	0.707LysLys: 0.707 ± 0.039	1.439LysLeu: 1.439 ± 0.037	0.359LysMet: 0.359 ± 0.019	0.445LysAsn: 0.445 ± 0.018	1.062LysPro: 1.062 ± 0.036	0.615LysGln: 0.615 ± 0.025	1.314LysArg: 1.314 ± 0.035	0.925LysSer: 0.925 ± 0.027	1.009LysThr: 1.009 ± 0.03	1.862LysVal: 1.862 ± 0.042	0.203LysTrp: 0.203 ± 0.013	0.404LysTyr: 0.404 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	15.756LeuAla: 15.756 ± 0.134	0.661LeuCys: 0.661 ± 0.023	6.963LeuAsp: 6.963 ± 0.085	5.289LeuGlu: 5.289 ± 0.074	2.49LeuPhe: 2.49 ± 0.049	9.947LeuGly: 9.947 ± 0.096	1.935LeuHis: 1.935 ± 0.043	3.121LeuIle: 3.121 ± 0.053	1.599LeuLys: 1.599 ± 0.037	10.614LeuLeu: 10.614 ± 0.134	1.614LeuMet: 1.614 ± 0.036	1.571LeuAsn: 1.571 ± 0.036	5.813LeuPro: 5.813 ± 0.062	2.128LeuGln: 2.128 ± 0.044	7.915LeuArg: 7.915 ± 0.106	4.969LeuSer: 4.969 ± 0.063	6.324LeuThr: 6.324 ± 0.078	10.219LeuVal: 10.219 ± 0.122	1.212LeuTrp: 1.212 ± 0.031	1.56LeuTyr: 1.56 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	2.352MetAla: 2.352 ± 0.046	0.122MetCys: 0.122 ± 0.009	0.86MetAsp: 0.86 ± 0.029	0.752MetGlu: 0.752 ± 0.026	0.442MetPhe: 0.442 ± 0.02	1.387MetGly: 1.387 ± 0.036	0.305MetHis: 0.305 ± 0.015	0.691MetIle: 0.691 ± 0.023	0.394MetLys: 0.394 ± 0.018	1.786MetLeu: 1.786 ± 0.035	0.339MetMet: 0.339 ± 0.016	0.385MetAsn: 0.385 ± 0.017	1.058MetPro: 1.058 ± 0.028	0.414MetGln: 0.414 ± 0.019	1.345MetArg: 1.345 ± 0.032	1.381MetSer: 1.381 ± 0.029	1.56MetThr: 1.56 ± 0.035	1.565MetVal: 1.565 ± 0.033	0.196MetTrp: 0.196 ± 0.012	0.281MetTyr: 0.281 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.221AsnAla: 2.221 ± 0.044	0.152AsnCys: 0.152 ± 0.019	1.013AsnAsp: 1.013 ± 0.035	0.849AsnGlu: 0.849 ± 0.029	0.462AsnPhe: 0.462 ± 0.022	1.74AsnGly: 1.74 ± 0.045	0.341AsnHis: 0.341 ± 0.016	0.599AsnIle: 0.599 ± 0.022	0.352AsnLys: 0.352 ± 0.016	1.796AsnLeu: 1.796 ± 0.04	0.285AsnMet: 0.285 ± 0.017	0.402AsnAsn: 0.402 ± 0.019	1.331AsnPro: 1.331 ± 0.037	0.512AsnGln: 0.512 ± 0.022	1.23AsnArg: 1.23 ± 0.035	0.742AsnSer: 0.742 ± 0.024	0.928AsnThr: 0.928 ± 0.033	1.451AsnVal: 1.451 ± 0.039	0.247AsnTrp: 0.247 ± 0.013	0.359AsnTyr: 0.359 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	8.447ProAla: 8.447 ± 0.116	0.294ProCys: 0.294 ± 0.017	4.428ProAsp: 4.428 ± 0.064	4.164ProGlu: 4.164 ± 0.065	1.496ProPhe: 1.496 ± 0.032	6.086ProGly: 6.086 ± 0.083	1.122ProHis: 1.122 ± 0.033	1.812ProIle: 1.812 ± 0.04	0.919ProLys: 0.919 ± 0.029	4.865ProLeu: 4.865 ± 0.066	0.972ProMet: 0.972 ± 0.031	0.877ProAsn: 0.877 ± 0.031	3.059ProPro: 3.059 ± 0.08	1.331ProGln: 1.331 ± 0.032	3.762ProArg: 3.762 ± 0.064	2.999ProSer: 2.999 ± 0.056	3.795ProThr: 3.795 ± 0.061	5.189ProVal: 5.189 ± 0.06	0.881ProTrp: 0.881 ± 0.025	1.161ProTyr: 1.161 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.356GlnAla: 3.356 ± 0.057	0.153GlnCys: 0.153 ± 0.01	1.106GlnAsp: 1.106 ± 0.029	1.278GlnGlu: 1.278 ± 0.039	0.658GlnPhe: 0.658 ± 0.022	1.931GlnGly: 1.931 ± 0.039	0.664GlnHis: 0.664 ± 0.025	1.087GlnIle: 1.087 ± 0.028	0.486GlnLys: 0.486 ± 0.022	2.757GlnLeu: 2.757 ± 0.05	0.54GlnMet: 0.54 ± 0.021	0.427GlnAsn: 0.427 ± 0.018	1.588GlnPro: 1.588 ± 0.041	1.053GlnGln: 1.053 ± 0.03	2.398GlnArg: 2.398 ± 0.043	1.132GlnSer: 1.132 ± 0.033	1.284GlnThr: 1.284 ± 0.034	2.726GlnVal: 2.726 ± 0.05	0.391GlnTrp: 0.391 ± 0.019	0.536GlnTyr: 0.536 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	9.658ArgAla: 9.658 ± 0.104	0.539ArgCys: 0.539 ± 0.021	4.413ArgAsp: 4.413 ± 0.067	4.32ArgGlu: 4.32 ± 0.074	2.299ArgPhe: 2.299 ± 0.045	5.978ArgGly: 5.978 ± 0.071	1.799ArgHis: 1.799 ± 0.041	3.217ArgIle: 3.217 ± 0.054	1.314ArgLys: 1.314 ± 0.039	8.092ArgLeu: 8.092 ± 0.106	1.725ArgMet: 1.725 ± 0.038	1.242ArgAsn: 1.242 ± 0.029	4.526ArgPro: 4.526 ± 0.076	1.995ArgGln: 1.995 ± 0.043	7.758ArgArg: 7.758 ± 0.128	4.139ArgSer: 4.139 ± 0.066	4.622ArgThr: 4.622 ± 0.074	6.5ArgVal: 6.5 ± 0.086	1.42ArgTrp: 1.42 ± 0.036	1.716ArgTyr: 1.716 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	6.583SerAla: 6.583 ± 0.077	0.342SerCys: 0.342 ± 0.017	2.88SerAsp: 2.88 ± 0.052	2.421SerGlu: 2.421 ± 0.038	1.634SerPhe: 1.634 ± 0.044	5.621SerGly: 5.621 ± 0.083	0.935SerHis: 0.935 ± 0.029	1.796SerIle: 1.796 ± 0.041	0.855SerLys: 0.855 ± 0.025	4.666SerLeu: 4.666 ± 0.055	1.084SerMet: 1.084 ± 0.029	0.906SerAsn: 0.906 ± 0.036	2.906SerPro: 2.906 ± 0.042	1.172SerGln: 1.172 ± 0.032	3.476SerArg: 3.476 ± 0.05	2.841SerSer: 2.841 ± 0.066	3.281SerThr: 3.281 ± 0.053	4.4SerVal: 4.4 ± 0.063	0.87SerTrp: 0.87 ± 0.033	1.278SerTyr: 1.278 ± 0.034	0.0SerXaa: 0.0 ± 0.0
Thr	7.907ThrAla: 7.907 ± 0.078	0.449ThrCys: 0.449 ± 0.019	3.756ThrAsp: 3.756 ± 0.067	3.151ThrGlu: 3.151 ± 0.056	1.818ThrPhe: 1.818 ± 0.04	6.295ThrGly: 6.295 ± 0.069	1.11ThrHis: 1.11 ± 0.03	2.185ThrIle: 2.185 ± 0.049	1.047ThrLys: 1.047 ± 0.029	5.872ThrLeu: 5.872 ± 0.071	0.983ThrMet: 0.983 ± 0.026	1.097ThrAsn: 1.097 ± 0.042	3.846ThrPro: 3.846 ± 0.061	1.277ThrGln: 1.277 ± 0.03	3.794ThrArg: 3.794 ± 0.056	3.214ThrSer: 3.214 ± 0.054	4.054ThrThr: 4.054 ± 0.071	5.896ThrVal: 5.896 ± 0.095	1.004ThrTrp: 1.004 ± 0.027	1.43ThrTyr: 1.43 ± 0.066	0.0ThrXaa: 0.0 ± 0.0
Val	13.505ValAla: 13.505 ± 0.128	0.652ValCys: 0.652 ± 0.024	6.271ValAsp: 6.271 ± 0.076	5.584ValGlu: 5.584 ± 0.08	2.304ValPhe: 2.304 ± 0.043	7.927ValGly: 7.927 ± 0.09	1.827ValHis: 1.827 ± 0.044	3.163ValIle: 3.163 ± 0.052	1.515ValLys: 1.515 ± 0.036	9.859ValLeu: 9.859 ± 0.103	1.467ValMet: 1.467 ± 0.038	1.661ValAsn: 1.661 ± 0.039	5.222ValPro: 5.222 ± 0.073	1.969ValGln: 1.969 ± 0.045	6.894ValArg: 6.894 ± 0.088	4.329ValSer: 4.329 ± 0.058	5.97ValThr: 5.97 ± 0.106	10.069ValVal: 10.069 ± 0.108	1.149ValTrp: 1.149 ± 0.029	1.461ValTyr: 1.461 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.721TrpAla: 1.721 ± 0.037	0.134TrpCys: 0.134 ± 0.01	0.811TrpAsp: 0.811 ± 0.026	0.721TrpGlu: 0.721 ± 0.02	0.534TrpPhe: 0.534 ± 0.02	1.108TrpGly: 1.108 ± 0.028	0.304TrpHis: 0.304 ± 0.015	0.558TrpIle: 0.558 ± 0.019	0.283TrpLys: 0.283 ± 0.015	1.829TrpLeu: 1.829 ± 0.04	0.316TrpMet: 0.316 ± 0.016	0.329TrpAsn: 0.329 ± 0.017	0.786TrpPro: 0.786 ± 0.032	0.517TrpGln: 0.517 ± 0.021	1.298TrpArg: 1.298 ± 0.036	0.991TrpSer: 0.991 ± 0.033	0.97TrpThr: 0.97 ± 0.029	1.244TrpVal: 1.244 ± 0.033	0.39TrpTrp: 0.39 ± 0.02	0.315TrpTyr: 0.315 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.601TyrAla: 2.601 ± 0.045	0.18TyrCys: 0.18 ± 0.013	1.667TyrAsp: 1.667 ± 0.036	1.014TyrGlu: 1.014 ± 0.03	0.661TyrPhe: 0.661 ± 0.021	2.081TyrGly: 2.081 ± 0.048	0.357TyrHis: 0.357 ± 0.017	0.412TyrIle: 0.412 ± 0.02	0.313TyrLys: 0.313 ± 0.016	2.241TyrLeu: 2.241 ± 0.043	0.211TyrMet: 0.211 ± 0.014	0.358TyrAsn: 0.358 ± 0.027	1.04TyrPro: 1.04 ± 0.03	0.57TyrGln: 0.57 ± 0.021	1.675TyrArg: 1.675 ± 0.039	0.912TyrSer: 0.912 ± 0.032	1.031TyrThr: 1.031 ± 0.038	1.864TyrVal: 1.864 ± 0.039	0.347TyrTrp: 0.347 ± 0.015	0.435TyrTyr: 0.435 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3866 proteins (1303206 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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