Prochlorococcus phage P-SSM2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Salacisavirus; Prochlorococcus virus PSSM2

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 334 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q58MS2|Q58MS2_BPPRM Uncharacterized protein OS=Prochlorococcus phage P-SSM2 OX=268746 GN=PCMG_00081 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84TPSNQLFADD11 pKa = 3.22IDD13 pKa = 4.03FLVDD17 pKa = 3.63EE18 pKa = 5.49LGMNANEE25 pKa = 4.62CDD27 pKa = 4.26DD28 pKa = 3.98VLHH31 pKa = 7.0ACDD34 pKa = 3.67EE35 pKa = 4.57LGGISAEE42 pKa = 4.23YY43 pKa = 9.88FCEE46 pKa = 3.85EE47 pKa = 4.72FIFEE51 pKa = 4.59TGDD54 pKa = 3.43PDD56 pKa = 6.4DD57 pKa = 4.73IDD59 pKa = 4.44RR60 pKa = 11.84LHH62 pKa = 7.32DD63 pKa = 4.37PNYY66 pKa = 11.08LKK68 pKa = 10.72INWGLNN74 pKa = 3.23

Molecular weight: 8.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q58M83|Q58M83_BPPRM High light inducible protein OS=Prochlorococcus phage P-SSM2 OX=268746 GN=hli03 PE=4 SV=1
MM1 pKa = 7.33NNKK4 pKa = 9.91QIFLRR9 pKa = 11.84ANGRR13 pKa = 11.84AAMIGFLVLCASYY26 pKa = 8.88ATTGNLIPGIVV37 pKa = 3.07

Molecular weight: 3.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

334

0

334

80255

24

5195

240.3

26.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.95 ± 0.141

1.077 ± 0.082

6.655 ± 0.111

6.109 ± 0.245

4.101 ± 0.087

8.447 ± 0.315

1.83 ± 0.076

6.611 ± 0.145

6.584 ± 0.298

6.954 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.011 ± 0.128

6.041 ± 0.149

4.241 ± 0.178

3.338 ± 0.084

3.673 ± 0.107

7.307 ± 0.213

7.151 ± 0.324

6.575 ± 0.184

1.163 ± 0.072

4.182 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski