Lactobacillus reuteri (strain DSM 20016)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Limosilactobacillus; Limosilactobacillus reuteri

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1865 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5VMQ7|A5VMQ7_LACRD Uncharacterized protein OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=Lreu_1894 PE=4 SV=1
MM1 pKa = 7.34SKK3 pKa = 10.29VLAAALGAAGALAVANTASADD24 pKa = 3.72TQVQVQSGDD33 pKa = 4.12TVWGFAQQYY42 pKa = 7.18ATTVDD47 pKa = 4.06SISTANQLADD57 pKa = 3.82PNVIYY62 pKa = 10.65VGQQLVIPSSAISTASAAATVAAPTATDD90 pKa = 3.2NATASQAAVEE100 pKa = 4.48TTEE103 pKa = 4.04TTAASTTPAVSTTNASNVADD123 pKa = 3.74QAQPTTVVSASDD135 pKa = 3.69NNTAAQVSVASQAPAQVSAASATISVAQTSAANTNNNVTTLAATTNTAAPTQTTYY190 pKa = 11.66AATEE194 pKa = 4.06AVATPQSNTTVQSTAAGQGNGSTANAVAVAQAQIGTPYY232 pKa = 10.33VWGGNQPGGFDD243 pKa = 3.67CSGLVQYY250 pKa = 11.04AYY252 pKa = 10.79GLGSNYY258 pKa = 8.15RR259 pKa = 11.84TTYY262 pKa = 9.33QQTNLGTHH270 pKa = 5.82QYY272 pKa = 10.66DD273 pKa = 3.25IQNAQSGDD281 pKa = 3.66LYY283 pKa = 11.19FWGPDD288 pKa = 3.1SAPYY292 pKa = 9.81HH293 pKa = 5.35VAIATGNGGYY303 pKa = 9.14IQAPTPGQNVQTGNINYY320 pKa = 6.95YY321 pKa = 9.24TPSFYY326 pKa = 10.49ISMNN330 pKa = 3.37

Molecular weight: 33.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5VHJ2|A5VHJ2_LACRD Peptidoglycan transpeptidase ErfK-YbiS-YhnG family OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=Lreu_0040 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.02KK9 pKa = 7.4RR10 pKa = 11.84HH11 pKa = 5.36RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 5.84GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1865

0

1865

559402

31

1443

299.9

33.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.707 ± 0.058

0.586 ± 0.014

5.888 ± 0.06

5.701 ± 0.067

4.106 ± 0.04

6.546 ± 0.058

2.33 ± 0.026

7.371 ± 0.063

6.678 ± 0.051

9.504 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.029

5.209 ± 0.043

3.698 ± 0.028

4.677 ± 0.055

4.289 ± 0.047

5.542 ± 0.047

5.822 ± 0.041

6.8 ± 0.042

1.078 ± 0.025

3.717 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski