Clostridium phage vB_CpeS-CP51

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9Q2L5|M9Q2L5_9CAUD Uncharacterized protein OS=Clostridium phage vB_CpeS-CP51 OX=1305708 GN=phiCP51_0041 PE=4 SV=1
MM1 pKa = 7.51VYY3 pKa = 10.56LISYY7 pKa = 9.1DD8 pKa = 3.85LNKK11 pKa = 10.08TGQDD15 pKa = 3.53YY16 pKa = 11.39NSLYY20 pKa = 10.58EE21 pKa = 4.36AIKK24 pKa = 10.82NSSTGVWFHH33 pKa = 6.39YY34 pKa = 10.63LDD36 pKa = 3.67STWIIKK42 pKa = 10.26SYY44 pKa = 11.21LSIQQVSDD52 pKa = 3.95NIKK55 pKa = 11.05SKK57 pKa = 10.46MDD59 pKa = 4.5DD60 pKa = 3.58NDD62 pKa = 3.78SLLVIEE68 pKa = 5.16VKK70 pKa = 10.71NNYY73 pKa = 9.23YY74 pKa = 10.73GWLPKK79 pKa = 10.36DD80 pKa = 2.66AWEE83 pKa = 4.02YY84 pKa = 11.02LRR86 pKa = 11.84TDD88 pKa = 3.09VFII91 pKa = 5.6

Molecular weight: 10.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9Q2L6|M9Q2L6_9CAUD Uncharacterized protein OS=Clostridium phage vB_CpeS-CP51 OX=1305708 GN=phiCP51_0046 PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 6.07SYY4 pKa = 11.06SSWLRR9 pKa = 11.84GQIANLLVGFMSSKK23 pKa = 10.49SSNLLLSAIKK33 pKa = 10.25FNIPHH38 pKa = 6.63RR39 pKa = 11.84RR40 pKa = 11.84GLHH43 pKa = 4.78

Molecular weight: 4.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

11843

39

2205

236.9

27.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.463 ± 0.502

1.064 ± 0.132

5.64 ± 0.276

7.954 ± 0.53

3.842 ± 0.202

5.624 ± 0.303

1.072 ± 0.12

9.128 ± 0.327

10.817 ± 0.56

8.072 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.246

7.625 ± 0.448

2.195 ± 0.194

3.014 ± 0.205

3.656 ± 0.273

6.578 ± 0.383

5.843 ± 0.451

5.176 ± 0.177

1.14 ± 0.12

3.909 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski