Polaribacter sp. Hel1_85

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter; unclassified Polaribacter

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3382 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A099XNY3|A0A099XNY3_9FLAO Cell division trigger factor OS=Polaribacter sp. Hel1_85 OX=1250005 GN=PHEL85_2926 PE=4 SV=1
MM1 pKa = 7.21PTGSFTFADD10 pKa = 3.83PNGTSNEE17 pKa = 3.89KK18 pKa = 10.44QIEE21 pKa = 4.29NNHH24 pKa = 5.85YY25 pKa = 10.67AVIDD29 pKa = 3.65PTHH32 pKa = 6.35IQDD35 pKa = 4.63AYY37 pKa = 10.06PSDD40 pKa = 5.52GYY42 pKa = 10.14WFWSGNIPPGVSSGVNSPDD61 pKa = 3.45TDD63 pKa = 3.36DD64 pKa = 4.25HH65 pKa = 5.8TTGDD69 pKa = 3.21INGAVMAINAGTTLDD84 pKa = 3.66YY85 pKa = 11.03LYY87 pKa = 11.08VRR89 pKa = 11.84SVSLNAGDD97 pKa = 5.12CYY99 pKa = 10.68RR100 pKa = 11.84ISYY103 pKa = 8.68WIYY106 pKa = 10.69LVNNDD111 pKa = 3.46AQIEE115 pKa = 4.21VDD117 pKa = 5.8LRR119 pKa = 11.84DD120 pKa = 3.48TSNQSVLNNDD130 pKa = 3.06NDD132 pKa = 3.89YY133 pKa = 9.21QTNLITIIDD142 pKa = 3.46NWAQYY147 pKa = 10.99SIDD150 pKa = 3.52FKK152 pKa = 11.74VPNTCTNTDD161 pKa = 3.88FEE163 pKa = 4.74VTLRR167 pKa = 11.84NDD169 pKa = 3.24YY170 pKa = 10.34PLNSGNDD177 pKa = 3.62YY178 pKa = 11.04YY179 pKa = 11.71VDD181 pKa = 4.8DD182 pKa = 4.46IFMEE186 pKa = 4.35KK187 pKa = 10.54LSTCTATEE195 pKa = 4.05VANCPLGVSQTDD207 pKa = 3.22KK208 pKa = 11.37DD209 pKa = 3.89GDD211 pKa = 4.71SIPDD215 pKa = 3.5MDD217 pKa = 7.2DD218 pKa = 3.68IDD220 pKa = 5.47DD221 pKa = 4.99DD222 pKa = 4.49NDD224 pKa = 4.89GILDD228 pKa = 3.82TLEE231 pKa = 4.75SNGNNPDD238 pKa = 3.3GDD240 pKa = 3.99EE241 pKa = 5.09DD242 pKa = 5.11GDD244 pKa = 4.4GIPNFKK250 pKa = 10.92DD251 pKa = 3.29NTDD254 pKa = 3.27GTLGNGSPGTDD265 pKa = 3.07YY266 pKa = 11.47TDD268 pKa = 3.62TNNDD272 pKa = 4.62GIPDD276 pKa = 3.82VYY278 pKa = 11.36DD279 pKa = 4.16FDD281 pKa = 7.03DD282 pKa = 5.42DD283 pKa = 5.38GISNHH288 pKa = 6.82LDD290 pKa = 3.11LDD292 pKa = 4.06ADD294 pKa = 3.61NDD296 pKa = 4.66GIYY299 pKa = 10.35DD300 pKa = 3.49ILEE303 pKa = 4.37AGGTDD308 pKa = 3.52SNNNGVADD316 pKa = 4.81DD317 pKa = 5.45LSDD320 pKa = 4.26LDD322 pKa = 4.15SDD324 pKa = 4.33GLVDD328 pKa = 5.88IYY330 pKa = 11.35DD331 pKa = 4.21DD332 pKa = 4.03TCQSPAIPASNYY344 pKa = 9.23YY345 pKa = 9.08ATDD348 pKa = 3.34VTASQHH354 pKa = 6.33FNNTNNALGAPSNTFSSGPTGWPGSNPFYY383 pKa = 10.92VVLDD387 pKa = 3.71FTEE390 pKa = 4.24TLTVGTEE397 pKa = 3.98LTIHH401 pKa = 7.01LGTNTGTASVRR412 pKa = 11.84VRR414 pKa = 11.84QTDD417 pKa = 2.99ASGTGDD423 pKa = 3.68TNYY426 pKa = 10.22VAYY429 pKa = 10.25NVTGTDD435 pKa = 3.49PTTFTYY441 pKa = 10.13TITYY445 pKa = 10.56SNTDD449 pKa = 3.52YY450 pKa = 11.31IKK452 pKa = 10.59IQTYY456 pKa = 9.83DD457 pKa = 3.38YY458 pKa = 9.96NVRR461 pKa = 11.84VYY463 pKa = 10.46GVEE466 pKa = 3.95YY467 pKa = 8.86GTAASGGEE475 pKa = 4.07DD476 pKa = 3.47CAGSALSPIEE486 pKa = 4.28TTLGTADD493 pKa = 4.53FLNLDD498 pKa = 4.2SDD500 pKa = 4.17GDD502 pKa = 3.98GCFDD506 pKa = 3.42VLEE509 pKa = 4.98AGFTDD514 pKa = 3.8ANIDD518 pKa = 3.71GEE520 pKa = 4.37IDD522 pKa = 3.38GTSYY526 pKa = 9.05STKK529 pKa = 10.47GIVIGSDD536 pKa = 3.28GYY538 pKa = 9.65TGTTSAVTDD547 pKa = 3.73NSNSAACQSLIDD559 pKa = 4.23SDD561 pKa = 4.67NDD563 pKa = 3.39GSTDD567 pKa = 5.51LDD569 pKa = 5.49DD570 pKa = 6.77LDD572 pKa = 5.83DD573 pKa = 5.79DD574 pKa = 4.9NDD576 pKa = 5.08GILDD580 pKa = 4.12TIEE583 pKa = 4.53NNQCGIGGNIVTTIFTEE600 pKa = 4.77DD601 pKa = 3.73FGTQNTSNGTKK612 pKa = 10.39SNTSEE617 pKa = 3.92YY618 pKa = 10.51TNYY621 pKa = 10.53NYY623 pKa = 11.06YY624 pKa = 9.44EE625 pKa = 4.57AEE627 pKa = 4.33VGTVPTDD634 pKa = 2.88WMDD637 pKa = 3.41GSSAPQSLQDD647 pKa = 3.29GRR649 pKa = 11.84YY650 pKa = 8.22TIFNDD655 pKa = 3.19IQYY658 pKa = 7.4TASWAATDD666 pKa = 3.3WQTIGDD672 pKa = 4.34HH673 pKa = 5.32TTGSATPSSGRR684 pKa = 11.84MAIFNANEE692 pKa = 4.14NIAGLEE698 pKa = 4.23FYY700 pKa = 10.84RR701 pKa = 11.84RR702 pKa = 11.84TLKK705 pKa = 11.01NIVAGAPINASYY717 pKa = 9.74WAMNIYY723 pKa = 6.9TTPGGSSVIKK733 pKa = 10.3PNITVQFIQRR743 pKa = 11.84GNEE746 pKa = 3.82VYY748 pKa = 10.71SFNSGDD754 pKa = 3.74IDD756 pKa = 3.7PVAAGSTAAWSQFSNQTVFIPTSNDD781 pKa = 3.38PIEE784 pKa = 5.02IIFINNTIGGGGNDD798 pKa = 4.32LAIDD802 pKa = 5.38DD803 pKa = 4.35ILITQSFCDD812 pKa = 3.95EE813 pKa = 5.17DD814 pKa = 5.49GDD816 pKa = 5.23GIPSYY821 pKa = 11.43LDD823 pKa = 3.83LDD825 pKa = 4.1SDD827 pKa = 4.02NDD829 pKa = 4.67GIPDD833 pKa = 3.67VIEE836 pKa = 4.66AGGTDD841 pKa = 3.55VNRR844 pKa = 11.84DD845 pKa = 3.22GLADD849 pKa = 4.82DD850 pKa = 6.0DD851 pKa = 5.42DD852 pKa = 5.9NNVNNFATNGIPTTAGTGLTATNTDD877 pKa = 3.51GDD879 pKa = 4.42SLPDD883 pKa = 3.76YY884 pKa = 11.21LDD886 pKa = 3.77IDD888 pKa = 4.59ADD890 pKa = 3.86NDD892 pKa = 4.72GIPDD896 pKa = 4.06NIEE899 pKa = 3.97AQSSNRR905 pKa = 11.84YY906 pKa = 8.31IAPSSVGATIIDD918 pKa = 3.97TNNNGVDD925 pKa = 3.76DD926 pKa = 4.49NYY928 pKa = 10.31EE929 pKa = 3.91TGSIVGLNPINTDD942 pKa = 2.98SSNDD946 pKa = 3.92TIPDD950 pKa = 3.79YY951 pKa = 11.4LDD953 pKa = 3.87SDD955 pKa = 4.24SDD957 pKa = 3.77NDD959 pKa = 4.62GINDD963 pKa = 3.46VLEE966 pKa = 4.87NGDD969 pKa = 3.76TDD971 pKa = 4.63NIASGLDD978 pKa = 3.39TDD980 pKa = 6.1GDD982 pKa = 4.15GLDD985 pKa = 4.48NAFDD989 pKa = 5.15DD990 pKa = 5.01NDD992 pKa = 3.91DD993 pKa = 3.79SSITGSTINDD1003 pKa = 3.5GLGIGNKK1010 pKa = 8.05VTDD1013 pKa = 3.68SSSLEE1018 pKa = 4.06SAFGDD1023 pKa = 3.51EE1024 pKa = 5.99DD1025 pKa = 5.13SDD1027 pKa = 4.27FNPGTGDD1034 pKa = 3.54LDD1036 pKa = 3.82YY1037 pKa = 11.0RR1038 pKa = 11.84DD1039 pKa = 3.93SFNPQGTPMITQVYY1053 pKa = 8.87QFGTEE1058 pKa = 3.45KK1059 pKa = 10.02WIEE1062 pKa = 3.75ITNISTTNSIEE1073 pKa = 3.89ANLIKK1078 pKa = 10.36IQLYY1082 pKa = 10.26LNKK1085 pKa = 10.1TGDD1088 pKa = 3.76QTGVTPDD1095 pKa = 3.01VTYY1098 pKa = 9.98TVTSALIPGQSIIIGNSANSIINTNAGAITVTNDD1132 pKa = 2.67ALTNIDD1138 pKa = 4.04GANDD1142 pKa = 5.59LITLSTTNDD1151 pKa = 3.06TTSFANRR1158 pKa = 11.84YY1159 pKa = 9.15DD1160 pKa = 3.97VIEE1163 pKa = 4.35SSSNKK1168 pKa = 8.88TSFVRR1173 pKa = 11.84IDD1175 pKa = 3.32EE1176 pKa = 4.4TLTPNSTYY1184 pKa = 10.07TVSEE1188 pKa = 3.87WAVFIDD1194 pKa = 5.24DD1195 pKa = 5.23ALDD1198 pKa = 4.13PYY1200 pKa = 11.32LLLGAGGAEE1209 pKa = 4.04RR1210 pKa = 11.84HH1211 pKa = 5.78PHH1213 pKa = 6.76DD1214 pKa = 4.65PLISEE1219 pKa = 4.65IEE1221 pKa = 4.14NANAQANIQLGLHH1234 pKa = 6.92RR1235 pKa = 11.84IDD1237 pKa = 2.96ITNRR1241 pKa = 11.84VAASGGSWDD1250 pKa = 3.43NGYY1253 pKa = 9.49PDD1255 pKa = 3.44RR1256 pKa = 11.84SRR1258 pKa = 11.84YY1259 pKa = 9.97VIIDD1263 pKa = 3.2EE1264 pKa = 5.21DD1265 pKa = 4.59YY1266 pKa = 11.13NHH1268 pKa = 5.9TTDD1271 pKa = 3.77RR1272 pKa = 11.84LSARR1276 pKa = 11.84KK1277 pKa = 8.39LTVNTSKK1284 pKa = 11.03KK1285 pKa = 10.69LSITDD1290 pKa = 3.35NLLAVTNDD1298 pKa = 2.71ILLNGEE1304 pKa = 3.75VRR1306 pKa = 11.84LIGSSQLIQTHH1317 pKa = 5.58TAASLVTGTGILLVDD1332 pKa = 4.0QNSTVPSKK1340 pKa = 11.0YY1341 pKa = 9.12RR1342 pKa = 11.84YY1343 pKa = 9.72NYY1345 pKa = 8.9MGSPVKK1351 pKa = 10.83NSTGAANYY1359 pKa = 7.9TVASIFKK1366 pKa = 10.53DD1367 pKa = 3.21GTNPTNHH1374 pKa = 6.59TGIINTNIAKK1384 pKa = 10.34DD1385 pKa = 3.41ITWIGGYY1392 pKa = 10.34DD1393 pKa = 4.14GDD1395 pKa = 4.15TTDD1398 pKa = 5.12PISLAEE1404 pKa = 3.95HH1405 pKa = 7.17WIYY1408 pKa = 10.59TYY1410 pKa = 11.06AANGGSRR1417 pKa = 11.84ASWTQKK1423 pKa = 9.85FSAGNIPNTDD1433 pKa = 2.71GFIFKK1438 pKa = 10.89GPGRR1442 pKa = 11.84AQNYY1446 pKa = 6.04TFSGIPKK1453 pKa = 9.8DD1454 pKa = 3.89GLITTSIGANEE1465 pKa = 4.57SYY1467 pKa = 11.06LVGNPFASAISVKK1480 pKa = 10.4EE1481 pKa = 4.02FIEE1484 pKa = 4.2NNNASISGTLYY1495 pKa = 9.34FWEE1498 pKa = 4.75HH1499 pKa = 6.47ASEE1502 pKa = 4.13QTTQEE1507 pKa = 4.2GSSVGHH1513 pKa = 6.24NFAGYY1518 pKa = 9.31IGGYY1522 pKa = 6.21ATRR1525 pKa = 11.84TIAMGVNAKK1534 pKa = 8.42TAANTGTVNTTLEE1547 pKa = 4.28AEE1549 pKa = 4.36NANTINGTSEE1559 pKa = 4.13QIVDD1563 pKa = 3.55NSVTIDD1569 pKa = 3.38VVRR1572 pKa = 11.84FNATNNFIEE1581 pKa = 4.74FEE1583 pKa = 4.41NIVKK1587 pKa = 10.44GVDD1590 pKa = 3.28TLKK1593 pKa = 10.35IRR1595 pKa = 11.84YY1596 pKa = 8.69KK1597 pKa = 11.02ADD1599 pKa = 3.42LDD1601 pKa = 3.85KK1602 pKa = 11.61NIILKK1607 pKa = 8.3TQGDD1611 pKa = 4.09VLKK1614 pKa = 9.49TINITLPQTSGEE1626 pKa = 3.95FSIFEE1631 pKa = 4.11IKK1633 pKa = 10.21HH1634 pKa = 5.35CAEE1637 pKa = 4.3PSDD1640 pKa = 4.58NITITSNDD1648 pKa = 3.39TNLLYY1653 pKa = 9.78IDD1655 pKa = 4.98HH1656 pKa = 7.25LNLYY1660 pKa = 10.21DD1661 pKa = 4.29GDD1663 pKa = 4.25GLISCVPNVGGDD1675 pKa = 3.37QITYY1679 pKa = 8.92TEE1681 pKa = 4.48PEE1683 pKa = 4.14PYY1685 pKa = 9.75IAIGQGFFVQGDD1697 pKa = 3.93NVDD1700 pKa = 3.47GGQIIFNNSQRR1711 pKa = 11.84EE1712 pKa = 4.13FKK1714 pKa = 10.68TEE1716 pKa = 3.96GTGSSVFLKK1725 pKa = 11.0SNAKK1729 pKa = 8.41KK1730 pKa = 7.32TTDD1733 pKa = 3.31NNSILTLPVIKK1744 pKa = 10.49LGMNFNSTDD1753 pKa = 3.58DD1754 pKa = 3.39NKK1756 pKa = 10.89KK1757 pKa = 7.8YY1758 pKa = 10.3HH1759 pKa = 5.74RR1760 pKa = 11.84QIGISFSNYY1769 pKa = 7.8TSFSYY1774 pKa = 11.01DD1775 pKa = 2.66KK1776 pKa = 11.04GYY1778 pKa = 10.63DD1779 pKa = 3.3AEE1781 pKa = 5.36IYY1783 pKa = 10.5DD1784 pKa = 3.83VGNTDD1789 pKa = 4.12VYY1791 pKa = 10.99WKK1793 pKa = 10.77FPDD1796 pKa = 3.99DD1797 pKa = 3.42EE1798 pKa = 5.0RR1799 pKa = 11.84KK1800 pKa = 10.01FIIAGVQNITDD1811 pKa = 3.9DD1812 pKa = 4.34LEE1814 pKa = 4.65VPLEE1818 pKa = 3.84ITMGYY1823 pKa = 9.53SGEE1826 pKa = 4.08IVLKK1830 pKa = 9.97VDD1832 pKa = 3.2EE1833 pKa = 4.99MKK1835 pKa = 10.95NISNNIYY1842 pKa = 8.86ITDD1845 pKa = 3.8KK1846 pKa = 10.7LTGNSYY1852 pKa = 11.06DD1853 pKa = 3.4ILNNKK1858 pKa = 9.14AMLNLEE1864 pKa = 3.92QGLYY1868 pKa = 9.14TDD1870 pKa = 4.25RR1871 pKa = 11.84FVLAFALTNALNINDD1886 pKa = 4.73EE1887 pKa = 4.37IIKK1890 pKa = 10.35QYY1892 pKa = 9.2ATVYY1896 pKa = 9.92VDD1898 pKa = 3.73HH1899 pKa = 7.14NNAHH1903 pKa = 6.69LVITKK1908 pKa = 9.1NQNINIKK1915 pKa = 9.72QVSLYY1920 pKa = 10.6NILGKK1925 pKa = 10.45KK1926 pKa = 9.06VGFWNIKK1933 pKa = 7.54EE1934 pKa = 4.13QNQKK1938 pKa = 8.93TKK1940 pKa = 11.06LSFNKK1945 pKa = 10.02RR1946 pKa = 11.84LATGLYY1952 pKa = 6.96ITKK1955 pKa = 10.1INTDD1959 pKa = 3.19KK1960 pKa = 11.51GQFSQKK1966 pKa = 10.32IIIEE1970 pKa = 4.13

Molecular weight:
212.62 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A099Y1P6|A0A099Y1P6_9FLAO Anthranilate synthase component II OS=Polaribacter sp. Hel1_85 OX=1250005 GN=PHEL85_2563 PE=4 SV=1
MM1 pKa = 7.79IDD3 pKa = 3.6NIRR6 pKa = 11.84HH7 pKa = 4.66FFEE10 pKa = 5.22RR11 pKa = 11.84NGFHH15 pKa = 6.25VSSRR19 pKa = 11.84LADD22 pKa = 4.89RR23 pKa = 11.84IGMRR27 pKa = 11.84ALNVRR32 pKa = 11.84LFFIYY37 pKa = 9.38VTFFTVGLSFGFYY50 pKa = 9.77LTMAFLFKK58 pKa = 10.7LKK60 pKa = 11.02DD61 pKa = 3.45MVHH64 pKa = 6.46TSRR67 pKa = 11.84SSVFDD72 pKa = 3.56LL73 pKa = 4.79

Molecular weight:
8.65 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3382

0

3382

1146162

37

3283

338.9

38.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.793 ± 0.037

0.692 ± 0.013

5.472 ± 0.045

6.578 ± 0.038

5.483 ± 0.036

6.257 ± 0.048

1.633 ± 0.021

8.491 ± 0.042

8.781 ± 0.065

9.044 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.99 ± 0.024

6.802 ± 0.052

3.113 ± 0.026

3.043 ± 0.019

3.007 ± 0.028

6.768 ± 0.038

5.965 ± 0.049

6.021 ± 0.029

1.037 ± 0.016

4.031 ± 0.027

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski