Achromobacter phage phiAxp-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G3EVK9|A0A0G3EVK9_9CAUD Uncharacterized protein OS=Achromobacter phage phiAxp-1 OX=1610509 GN=RU58_00018 PE=4 SV=1
MM1 pKa = 7.16NVITVTEE8 pKa = 4.03LMARR12 pKa = 11.84LRR14 pKa = 11.84EE15 pKa = 3.97ILNEE19 pKa = 4.24HH20 pKa = 6.48GDD22 pKa = 3.7IPVVVADD29 pKa = 4.59SLNDD33 pKa = 3.38ATPPQVQVEE42 pKa = 4.27YY43 pKa = 10.65GVAIILL49 pKa = 4.0

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G3EYI6|A0A0G3EYI6_9CAUD Scaffold protein OS=Achromobacter phage phiAxp-1 OX=1610509 GN=RU58_00043 PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 6.35WITQIPGPVGILGALLAVVMFYY24 pKa = 9.85VTGRR28 pKa = 11.84RR29 pKa = 11.84SGRR32 pKa = 11.84ADD34 pKa = 3.38AEE36 pKa = 4.27RR37 pKa = 11.84EE38 pKa = 4.1HH39 pKa = 6.11QRR41 pKa = 11.84QKK43 pKa = 11.37AEE45 pKa = 3.82EE46 pKa = 3.89ATEE49 pKa = 3.81QVRR52 pKa = 11.84NINRR56 pKa = 11.84TVGEE60 pKa = 4.15SNARR64 pKa = 11.84SEE66 pKa = 4.28ANRR69 pKa = 11.84RR70 pKa = 11.84LDD72 pKa = 3.28GAGIRR77 pKa = 11.84DD78 pKa = 3.77RR79 pKa = 11.84MRR81 pKa = 11.84NDD83 pKa = 2.97YY84 pKa = 10.75RR85 pKa = 11.84RR86 pKa = 3.34

Molecular weight: 9.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

14277

30

1331

223.1

24.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.002 ± 0.511

0.805 ± 0.104

5.442 ± 0.205

6.122 ± 0.332

3.81 ± 0.146

7.978 ± 0.422

1.632 ± 0.147

5.309 ± 0.213

5.197 ± 0.385

7.277 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.257 ± 0.14

4.721 ± 0.207

4.826 ± 0.27

3.964 ± 0.27

5.954 ± 0.25

5.61 ± 0.272

6.045 ± 0.248

7.004 ± 0.238

1.429 ± 0.121

3.614 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski