Sphingobacterium sp. ML3W

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium; unclassified Sphingobacterium

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4058 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088EZZ5|A0A088EZZ5_9SPHI Thymidylate synthase OS=Sphingobacterium sp. ML3W OX=1538644 GN=KO02_16490 PE=4 SV=1
MM1 pKa = 7.56VIPNSLADD9 pKa = 5.25AEE11 pKa = 5.03LLLNDD16 pKa = 4.21YY17 pKa = 10.23STMNTGYY24 pKa = 9.4PVYY27 pKa = 10.79GEE29 pKa = 4.66LSADD33 pKa = 3.54EE34 pKa = 4.86YY35 pKa = 11.6YY36 pKa = 11.08VALEE40 pKa = 4.43TFDD43 pKa = 4.6GMLDD47 pKa = 3.77FDD49 pKa = 4.94QRR51 pKa = 11.84NTYY54 pKa = 7.71TWMDD58 pKa = 3.92IIYY61 pKa = 10.29DD62 pKa = 5.43DD63 pKa = 3.64VAQWQRR69 pKa = 11.84PYY71 pKa = 10.97KK72 pKa = 10.62AVFNANQALEE82 pKa = 4.4IINNNSADD90 pKa = 3.59TKK92 pKa = 10.87IDD94 pKa = 3.12IKK96 pKa = 10.96KK97 pKa = 10.48

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088EUE5|A0A088EUE5_9SPHI Uncharacterized protein OS=Sphingobacterium sp. ML3W OX=1538644 GN=KO02_04745 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.14RR3 pKa = 11.84IKK5 pKa = 10.03HH6 pKa = 5.7IKK8 pKa = 9.63LIAIAIFSILFFANPNTTFAQRR30 pKa = 11.84GYY32 pKa = 11.06NDD34 pKa = 3.57YY35 pKa = 11.06QSGVSFQMFYY45 pKa = 11.45DD46 pKa = 3.88EE47 pKa = 4.67LAPYY51 pKa = 9.74GDD53 pKa = 3.28WVNDD57 pKa = 3.78RR58 pKa = 11.84EE59 pKa = 4.53HH60 pKa = 8.17GYY62 pKa = 8.18MWIPDD67 pKa = 3.92VGPNFQPYY75 pKa = 8.14STNGYY80 pKa = 6.2WTMTEE85 pKa = 4.29YY86 pKa = 11.42GNTWVSNYY94 pKa = 8.74SWGWAPFHH102 pKa = 6.16YY103 pKa = 10.38GRR105 pKa = 11.84WEE107 pKa = 3.81YY108 pKa = 10.92NNNYY112 pKa = 7.86GWAWTPDD119 pKa = 4.17YY120 pKa = 10.5EE121 pKa = 5.28WGPAWVNWRR130 pKa = 11.84QGSGYY135 pKa = 10.24YY136 pKa = 10.3GWAPLGINISINLPMNLWVFVGSSNIYY163 pKa = 9.4SNRR166 pKa = 11.84LDD168 pKa = 3.17RR169 pKa = 11.84YY170 pKa = 8.25YY171 pKa = 11.23VHH173 pKa = 7.11PRR175 pKa = 11.84NYY177 pKa = 10.47NNFYY181 pKa = 11.13NKK183 pKa = 7.88TTIINNTIIVNNRR196 pKa = 11.84NYY198 pKa = 10.46YY199 pKa = 9.53GGPRR203 pKa = 11.84RR204 pKa = 11.84SDD206 pKa = 3.05IEE208 pKa = 3.95RR209 pKa = 11.84STGKK213 pKa = 10.05RR214 pKa = 11.84VSVRR218 pKa = 11.84NINNSGRR225 pKa = 11.84AGTSRR230 pKa = 11.84VTKK233 pKa = 10.67NSIDD237 pKa = 3.52MYY239 pKa = 10.68RR240 pKa = 11.84PNIDD244 pKa = 3.24RR245 pKa = 11.84NSRR248 pKa = 11.84TDD250 pKa = 3.19ARR252 pKa = 11.84PSRR255 pKa = 11.84IAEE258 pKa = 4.02ASSRR262 pKa = 11.84TRR264 pKa = 11.84NNNTSLQNRR273 pKa = 11.84NDD275 pKa = 3.43KK276 pKa = 10.92VISDD280 pKa = 4.25RR281 pKa = 11.84DD282 pKa = 3.62NMTSTRR288 pKa = 11.84GNRR291 pKa = 11.84EE292 pKa = 3.65LYY294 pKa = 10.06IDD296 pKa = 3.65NKK298 pKa = 10.83GNATVRR304 pKa = 11.84SGSNNSTTTRR314 pKa = 11.84NNDD317 pKa = 2.68RR318 pKa = 11.84AANRR322 pKa = 11.84NQNSSNVTGSSRR334 pKa = 11.84TRR336 pKa = 11.84DD337 pKa = 3.35NSNATVNNSSTTGNARR353 pKa = 11.84TEE355 pKa = 4.49TNSSSVRR362 pKa = 11.84TDD364 pKa = 2.91RR365 pKa = 11.84TRR367 pKa = 11.84GNTSSSKK374 pKa = 10.04QEE376 pKa = 4.16EE377 pKa = 4.34IRR379 pKa = 11.84KK380 pKa = 9.71SGQPVQGQATRR391 pKa = 11.84SSRR394 pKa = 11.84TTQTQPARR402 pKa = 11.84NEE404 pKa = 3.79QPAKK408 pKa = 10.35VQSTSNDD415 pKa = 2.69ARR417 pKa = 11.84TSRR420 pKa = 11.84PAQGQQSRR428 pKa = 11.84SAQPAKK434 pKa = 10.46VQSSTSNDD442 pKa = 2.72ARR444 pKa = 11.84TSRR447 pKa = 11.84PAQGQQTRR455 pKa = 11.84SAQPARR461 pKa = 11.84VQSSTTDD468 pKa = 3.04RR469 pKa = 11.84GSSSSRR475 pKa = 11.84SSSSTVEE482 pKa = 4.03RR483 pKa = 11.84NTSQSEE489 pKa = 4.19NNRR492 pKa = 11.84SSGSRR497 pKa = 11.84TRR499 pKa = 3.72

Molecular weight: 56.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4058

0

4058

1400076

38

2700

345.0

38.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.857 ± 0.041

0.731 ± 0.011

5.566 ± 0.028

6.093 ± 0.038

4.95 ± 0.027

6.529 ± 0.039

1.9 ± 0.015

7.73 ± 0.039

7.242 ± 0.035

9.57 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.336 ± 0.018

5.75 ± 0.034

3.492 ± 0.021

3.88 ± 0.025

3.831 ± 0.023

6.536 ± 0.031

5.485 ± 0.024

6.16 ± 0.026

1.166 ± 0.015

4.197 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski