Streptomyces scopuliridis RB72

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces scopuliridis

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6830 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T7T3S4|A0A2T7T3S4_9ACTN Uncharacterized protein OS=Streptomyces scopuliridis RB72 OX=1440053 GN=Y717_33270 PE=4 SV=1
MM1 pKa = 7.01TAGTGGAVLAFPLLAATTAEE21 pKa = 4.37AAPAAHH27 pKa = 6.38TAATAKK33 pKa = 9.19VTLTPAAAVQQDD45 pKa = 3.71TPATYY50 pKa = 10.52SVVWGDD56 pKa = 4.14SLSKK60 pKa = 10.66IADD63 pKa = 3.83TYY65 pKa = 10.99SLSGGWQQLYY75 pKa = 10.49EE76 pKa = 4.21NNRR79 pKa = 11.84DD80 pKa = 3.74VIGANPSLIHH90 pKa = 6.89PGLTLTLGTEE100 pKa = 4.67SVSQAPAEE108 pKa = 4.3STADD112 pKa = 3.43EE113 pKa = 4.61SATADD118 pKa = 3.16EE119 pKa = 4.57SAAVDD124 pKa = 3.94EE125 pKa = 4.89SASADD130 pKa = 3.35TGATATQAAAKK141 pKa = 9.4TYY143 pKa = 10.62TDD145 pKa = 5.76DD146 pKa = 3.87LDD148 pKa = 3.47GWIRR152 pKa = 11.84EE153 pKa = 4.41ALDD156 pKa = 3.04VMAVHH161 pKa = 7.44GIPGSYY167 pKa = 10.37DD168 pKa = 3.31GIYY171 pKa = 9.79RR172 pKa = 11.84NIMRR176 pKa = 11.84EE177 pKa = 3.86SSGNPTAINNWDD189 pKa = 3.51SNAAAGIPSKK199 pKa = 11.13GLLQVIDD206 pKa = 3.93PTFQAYY212 pKa = 8.64HH213 pKa = 5.83VPGTSTDD220 pKa = 3.22SYY222 pKa = 11.62DD223 pKa = 3.31PVANITAACNYY234 pKa = 9.87AADD237 pKa = 4.59RR238 pKa = 11.84YY239 pKa = 10.99GSIDD243 pKa = 3.83NVNGPYY249 pKa = 10.54

Molecular weight: 25.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T7T3R3|A0A2T7T3R3_9ACTN Protein tyrosine phosphatase OS=Streptomyces scopuliridis RB72 OX=1440053 GN=Y717_35035 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.48GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6830

0

6830

2275614

27

9534

333.2

35.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.429 ± 0.051

0.733 ± 0.008

5.957 ± 0.025

5.764 ± 0.031

2.754 ± 0.017

9.619 ± 0.028

2.168 ± 0.013

3.354 ± 0.021

2.194 ± 0.028

10.326 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.735 ± 0.012

1.831 ± 0.019

6.047 ± 0.029

2.693 ± 0.017

7.959 ± 0.033

5.284 ± 0.023

6.223 ± 0.03

8.309 ± 0.032

1.492 ± 0.012

2.129 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski