Alphaproteobacteria phage PhiJL001

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Yuavirus; Alphaproteobacteria virus phiJl001

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5DN34|Q5DN34_9CAUD Gp71 OS=Alphaproteobacteria phage PhiJL001 OX=2681607 GN=JL001p71 PE=4 SV=1
MM1 pKa = 7.48SLGTAYY7 pKa = 10.3GGSTTSSGGGVDD19 pKa = 3.66TTVEE23 pKa = 4.22TANSSGYY30 pKa = 9.68MYY32 pKa = 10.71LQGDD36 pKa = 4.06ATTEE40 pKa = 3.88GSVRR44 pKa = 11.84FNASSDD50 pKa = 2.72IGAGVFEE57 pKa = 4.5KK58 pKa = 10.92LEE60 pKa = 4.18NGIWTANTLEE70 pKa = 4.32MGVDD74 pKa = 3.73SLFLGPRR81 pKa = 11.84TGLASIGEE89 pKa = 4.27YY90 pKa = 10.76LSVEE94 pKa = 4.02NSNIPDD100 pKa = 3.36ARR102 pKa = 11.84RR103 pKa = 11.84LFPYY107 pKa = 9.8TVFNGTTIDD116 pKa = 3.99DD117 pKa = 4.57LATIPYY123 pKa = 7.9VTAHH127 pKa = 6.48LTNVVSVPFDD137 pKa = 3.63SDD139 pKa = 3.42EE140 pKa = 4.42TTGTSYY146 pKa = 11.02SWTFTGTVNAMLKK159 pKa = 10.4NIYY162 pKa = 9.89IKK164 pKa = 10.21IGSVAPTDD172 pKa = 3.5TVRR175 pKa = 11.84FFVRR179 pKa = 11.84DD180 pKa = 3.34NDD182 pKa = 3.73EE183 pKa = 4.09NGQIIFDD190 pKa = 3.75EE191 pKa = 4.88SYY193 pKa = 9.82PASAFAANSTVALQTTGFFHH213 pKa = 6.93NKK215 pKa = 9.74VGDD218 pKa = 3.77TYY220 pKa = 11.46YY221 pKa = 10.28STFEE225 pKa = 3.89SSQPFSILGSAALQLPYY242 pKa = 10.66FEE244 pKa = 5.17VDD246 pKa = 2.61ASFIRR251 pKa = 11.84DD252 pKa = 3.35DD253 pKa = 4.6RR254 pKa = 11.84LWQASPWEE262 pKa = 4.31AGTYY266 pKa = 10.42AVGDD270 pKa = 3.7YY271 pKa = 10.48VIEE274 pKa = 4.16GRR276 pKa = 11.84VPYY279 pKa = 10.05ICVVAGAQATDD290 pKa = 3.88FATNSTSWKK299 pKa = 9.13PINGYY304 pKa = 10.31DD305 pKa = 3.72FDD307 pKa = 4.99DD308 pKa = 3.46WVTNYY313 pKa = 7.48GTRR316 pKa = 11.84AILDD320 pKa = 3.68EE321 pKa = 4.75NYY323 pKa = 11.2DD324 pKa = 4.23LIIDD328 pKa = 4.08EE329 pKa = 4.66ATLEE333 pKa = 4.14PVVLEE338 pKa = 4.47GTT340 pKa = 3.79

Molecular weight: 36.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5DN44|Q5DN44_9CAUD Gp61 OS=Alphaproteobacteria phage PhiJL001 OX=2681607 GN=JL001p61 PE=4 SV=1
MM1 pKa = 7.37PTDD4 pKa = 3.67DD5 pKa = 5.34QIPGMPDD12 pKa = 2.81NVNEE16 pKa = 4.05EE17 pKa = 4.22WLDD20 pKa = 3.31AMIRR24 pKa = 11.84HH25 pKa = 5.89QIGLLRR31 pKa = 11.84VSGRR35 pKa = 11.84VRR37 pKa = 11.84QRR39 pKa = 11.84IFEE42 pKa = 4.19ILDD45 pKa = 3.15ATEE48 pKa = 5.17RR49 pKa = 11.84DD50 pKa = 3.13IADD53 pKa = 3.44QIKK56 pKa = 10.26RR57 pKa = 11.84RR58 pKa = 11.84LAKK61 pKa = 10.25GATTARR67 pKa = 11.84LEE69 pKa = 4.12ALIKK73 pKa = 10.29AVRR76 pKa = 11.84ALRR79 pKa = 11.84SEE81 pKa = 3.99AWKK84 pKa = 10.6KK85 pKa = 9.79SAEE88 pKa = 4.01VWRR91 pKa = 11.84EE92 pKa = 3.48EE93 pKa = 4.03MLSVARR99 pKa = 11.84EE100 pKa = 3.89EE101 pKa = 4.46PKK103 pKa = 10.69FLSQALRR110 pKa = 11.84TVSPVQLDD118 pKa = 3.83LTLPAADD125 pKa = 4.57LLQSIVTTRR134 pKa = 11.84PFEE137 pKa = 4.33GQTMRR142 pKa = 11.84QWASNIRR149 pKa = 11.84NSDD152 pKa = 3.01LRR154 pKa = 11.84RR155 pKa = 11.84IEE157 pKa = 3.65RR158 pKa = 11.84AIRR161 pKa = 11.84IGVTQGEE168 pKa = 4.52PTNAIARR175 pKa = 11.84RR176 pKa = 11.84VIGTVRR182 pKa = 11.84QRR184 pKa = 11.84GRR186 pKa = 11.84DD187 pKa = 3.16GVTQITRR194 pKa = 11.84RR195 pKa = 11.84NAEE198 pKa = 4.34AITRR202 pKa = 11.84TAINGIANQAKK213 pKa = 10.05RR214 pKa = 11.84EE215 pKa = 4.07FYY217 pKa = 10.23KK218 pKa = 11.21ANATLFEE225 pKa = 4.19EE226 pKa = 4.97EE227 pKa = 4.96IYY229 pKa = 11.05VATLDD234 pKa = 4.0SRR236 pKa = 11.84TTPICRR242 pKa = 11.84SLDD245 pKa = 3.45GQRR248 pKa = 11.84FAIGEE253 pKa = 4.24GPIPPLHH260 pKa = 6.04FNCRR264 pKa = 11.84SLRR267 pKa = 11.84VAAIDD272 pKa = 3.52GDD274 pKa = 4.63AIGSRR279 pKa = 11.84PQRR282 pKa = 11.84QFTQRR287 pKa = 11.84QLLRR291 pKa = 11.84EE292 pKa = 4.15YY293 pKa = 10.92ARR295 pKa = 11.84SRR297 pKa = 11.84GIKK300 pKa = 10.2APTRR304 pKa = 11.84RR305 pKa = 11.84ADD307 pKa = 3.39LPRR310 pKa = 11.84GHH312 pKa = 6.92KK313 pKa = 10.98GEE315 pKa = 4.23FDD317 pKa = 3.17SFARR321 pKa = 11.84TRR323 pKa = 11.84IRR325 pKa = 11.84QLTGTVDD332 pKa = 3.39AKK334 pKa = 11.08VSYY337 pKa = 10.41QDD339 pKa = 2.69WLTRR343 pKa = 11.84QSTEE347 pKa = 3.74FQDD350 pKa = 4.5DD351 pKa = 3.94VLGKK355 pKa = 8.43TRR357 pKa = 11.84ARR359 pKa = 11.84LFRR362 pKa = 11.84KK363 pKa = 10.01GGLRR367 pKa = 11.84LDD369 pKa = 3.51RR370 pKa = 11.84FVNRR374 pKa = 11.84QGDD377 pKa = 4.71EE378 pKa = 3.9IPLADD383 pKa = 4.1LARR386 pKa = 11.84RR387 pKa = 11.84EE388 pKa = 4.11TSAFRR393 pKa = 11.84AAGLDD398 pKa = 3.66PEE400 pKa = 5.61DD401 pKa = 3.89FTT403 pKa = 5.87

Molecular weight: 45.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

20205

29

1152

224.5

24.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.112 ± 0.361

0.97 ± 0.112

6.686 ± 0.224

6.36 ± 0.271

4.078 ± 0.19

8.523 ± 0.33

1.881 ± 0.204

4.85 ± 0.182

4.172 ± 0.348

7.869 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.199

4.068 ± 0.18

4.147 ± 0.173

3.89 ± 0.186

6.3 ± 0.304

5.914 ± 0.259

7.157 ± 0.468

7.176 ± 0.164

1.891 ± 0.155

2.549 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski