Subdoligranulum sp. CAG:314

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Subdoligranulum; environmental samples

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1341 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7K8I9|R7K8I9_9FIRM T5orf172 domain-containing protein OS=Subdoligranulum sp. CAG:314 OX=1262970 GN=BN603_00829 PE=4 SV=1
MM1 pKa = 7.15NRR3 pKa = 11.84IAMVIGSWGSYY14 pKa = 8.56NEE16 pKa = 4.16CNEE19 pKa = 4.02RR20 pKa = 11.84ALGSKK25 pKa = 8.98WLDD28 pKa = 3.22FDD30 pKa = 6.61DD31 pKa = 4.87YY32 pKa = 11.81DD33 pKa = 4.04DD34 pKa = 3.46WDD36 pKa = 4.35EE37 pKa = 5.11IIEE40 pKa = 4.09EE41 pKa = 4.31LEE43 pKa = 4.13KK44 pKa = 11.21EE45 pKa = 4.3GFEE48 pKa = 4.87LDD50 pKa = 4.77GIDD53 pKa = 4.71EE54 pKa = 4.32EE55 pKa = 6.19LFVQDD60 pKa = 4.32IEE62 pKa = 5.15GIPTDD67 pKa = 4.73SEE69 pKa = 4.63DD70 pKa = 3.95WDD72 pKa = 3.68NTHH75 pKa = 6.87PKK77 pKa = 10.29EE78 pKa = 3.85IFEE81 pKa = 5.25LIKK84 pKa = 10.99SSGVLNDD91 pKa = 3.64NYY93 pKa = 10.68KK94 pKa = 10.45HH95 pKa = 6.66EE96 pKa = 4.23IMEE99 pKa = 4.57AYY101 pKa = 10.45CEE103 pKa = 4.06VRR105 pKa = 11.84SFDD108 pKa = 3.04EE109 pKa = 4.02WADD112 pKa = 3.41LVEE115 pKa = 5.02RR116 pKa = 11.84YY117 pKa = 10.03GEE119 pKa = 4.0NWDD122 pKa = 4.72DD123 pKa = 5.98DD124 pKa = 4.22IIFYY128 pKa = 10.34KK129 pKa = 9.55GQDD132 pKa = 3.65MYY134 pKa = 11.74DD135 pKa = 3.1VAYY138 pKa = 10.99NLVNEE143 pKa = 4.89CYY145 pKa = 10.29DD146 pKa = 3.77LEE148 pKa = 5.46KK149 pKa = 10.63MLGNLASYY157 pKa = 10.89FDD159 pKa = 4.38YY160 pKa = 11.37NAFARR165 pKa = 11.84DD166 pKa = 3.76LSFDD170 pKa = 3.88GYY172 pKa = 11.07SEE174 pKa = 4.22TEE176 pKa = 3.72RR177 pKa = 11.84GVIEE181 pKa = 4.04IRR183 pKa = 3.52

Molecular weight: 21.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7K6F9|R7K6F9_9FIRM Toxin-antitoxin system antitoxin component PHD family OS=Subdoligranulum sp. CAG:314 OX=1262970 GN=BN603_00034 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.11KK9 pKa = 7.52RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 6.0GFRR19 pKa = 11.84KK20 pKa = 9.68RR21 pKa = 11.84MKK23 pKa = 8.11STGGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 9.67GRR39 pKa = 11.84HH40 pKa = 5.38KK41 pKa = 10.87LSAA44 pKa = 3.8

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1341

0

1341

446468

32

2832

332.9

36.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.493 ± 0.066

1.694 ± 0.035

5.762 ± 0.058

7.132 ± 0.062

4.841 ± 0.049

7.287 ± 0.061

1.222 ± 0.022

6.2 ± 0.057

7.056 ± 0.076

9.398 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.034

4.57 ± 0.044

3.104 ± 0.032

2.485 ± 0.03

4.627 ± 0.065

6.279 ± 0.061

5.402 ± 0.076

7.663 ± 0.055

0.76 ± 0.022

3.689 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski