Aquabacterium sp. NJ1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Aquabacterium; unclassified Aquabacterium

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3620 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0DWL6|A0A0A0DWL6_9BURK Exosortase OS=Aquabacterium sp. NJ1 OX=1538295 GN=JY96_15445 PE=4 SV=1
MM1 pKa = 7.76EE2 pKa = 4.42STMTASVGSDD12 pKa = 3.55MPPPAPLSFSAAAAAKK28 pKa = 9.7VSEE31 pKa = 4.85LIADD35 pKa = 4.01EE36 pKa = 5.17GNPQLKK42 pKa = 10.1LRR44 pKa = 11.84LYY46 pKa = 8.65VTGGGCSGFSYY57 pKa = 10.66GFAFDD62 pKa = 4.56DD63 pKa = 4.64QLNEE67 pKa = 4.09DD68 pKa = 4.36DD69 pKa = 4.37TRR71 pKa = 11.84IDD73 pKa = 3.42KK74 pKa = 11.21DD75 pKa = 3.72GVTMVVDD82 pKa = 4.53AMSLQYY88 pKa = 11.66LMGADD93 pKa = 3.35IDD95 pKa = 4.33YY96 pKa = 11.15EE97 pKa = 5.37DD98 pKa = 4.32GLEE101 pKa = 3.97GSRR104 pKa = 11.84FVIHH108 pKa = 6.53NPNAQTTCGCGSSFSMM124 pKa = 5.09

Molecular weight: 13.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0DP73|A0A0A0DP73_9BURK Formate transporter OS=Aquabacterium sp. NJ1 OX=1538295 GN=JY96_08485 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.67TKK25 pKa = 10.48GGRR28 pKa = 11.84KK29 pKa = 9.03VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.67GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LALL44 pKa = 3.85

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3620

0

3620

1215807

37

9543

335.9

36.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.056 ± 0.065

0.941 ± 0.018

5.376 ± 0.034

5.104 ± 0.045

3.379 ± 0.029

8.087 ± 0.045

2.435 ± 0.029

4.392 ± 0.03

3.655 ± 0.04

10.669 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.666 ± 0.022

2.792 ± 0.032

5.131 ± 0.042

4.532 ± 0.035

6.262 ± 0.048

5.662 ± 0.038

5.424 ± 0.069

7.631 ± 0.033

1.541 ± 0.019

2.263 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski