Pseudolabrys sp. GY_H

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Xanthobacteraceae; Pseudolabrys; unclassified Pseudolabrys

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3838 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371B8F8|A0A371B8F8_9RHIZ LysR family transcriptional regulator OS=Pseudolabrys sp. GY_H OX=2292256 GN=DXH78_03405 PE=3 SV=1
MM1 pKa = 7.37TYY3 pKa = 10.71VVTEE7 pKa = 3.8NCIKK11 pKa = 10.19CKK13 pKa = 10.76YY14 pKa = 7.69MDD16 pKa = 4.28CVEE19 pKa = 4.32VCPVDD24 pKa = 4.15CFYY27 pKa = 11.35EE28 pKa = 4.72GDD30 pKa = 3.66NMLVIHH36 pKa = 7.26PDD38 pKa = 2.97EE39 pKa = 5.88CIDD42 pKa = 4.44CGVCEE47 pKa = 4.63PEE49 pKa = 5.02CPADD53 pKa = 4.4AIKK56 pKa = 10.54PDD58 pKa = 4.01TEE60 pKa = 4.58PGLEE64 pKa = 4.71KK65 pKa = 10.36FLEE68 pKa = 4.44LNAEE72 pKa = 4.38YY73 pKa = 10.87AKK75 pKa = 9.16TWPNITVKK83 pKa = 10.36RR84 pKa = 11.84DD85 pKa = 3.4PPADD89 pKa = 3.63AKK91 pKa = 11.19DD92 pKa = 3.32WDD94 pKa = 4.08GVADD98 pKa = 3.74KK99 pKa = 11.08LEE101 pKa = 4.2KK102 pKa = 10.61HH103 pKa = 6.4FSPKK107 pKa = 9.98PGEE110 pKa = 4.2GDD112 pKa = 3.05

Molecular weight: 12.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371B7R8|A0A371B7R8_9RHIZ DUF4212 domain-containing protein OS=Pseudolabrys sp. GY_H OX=2292256 GN=DXH78_02920 PE=4 SV=1
MM1 pKa = 8.04KK2 pKa = 9.97IRR4 pKa = 11.84NSLKK8 pKa = 9.99SLRR11 pKa = 11.84GRR13 pKa = 11.84HH14 pKa = 5.55RR15 pKa = 11.84NNKK18 pKa = 8.48LVRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 10.37GRR25 pKa = 11.84VFIINKK31 pKa = 6.32TNPRR35 pKa = 11.84FKK37 pKa = 10.75ARR39 pKa = 11.84QGG41 pKa = 3.28

Molecular weight: 4.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3838

0

3838

1211684

29

5984

315.7

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.739 ± 0.05

0.811 ± 0.013

5.538 ± 0.031

5.225 ± 0.039

3.77 ± 0.028

8.465 ± 0.047

1.921 ± 0.019

5.452 ± 0.026

4.065 ± 0.038

9.716 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.58 ± 0.018

2.786 ± 0.031

5.248 ± 0.036

3.016 ± 0.023

6.77 ± 0.05

5.215 ± 0.035

5.391 ± 0.041

7.744 ± 0.033

1.247 ± 0.016

2.3 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski