Bradyrhizobium sp. (strain ORS 278)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6701 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A4Z140|A4Z140_BRASO Putative Adenylate cyclase OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=BRADO6221 PE=4 SV=1
MM1 pKa = 7.5GSSGLTTTPSDD12 pKa = 2.98IVNRR16 pKa = 11.84DD17 pKa = 3.35FADD20 pKa = 4.05LQQPWMASFPQYY32 pKa = 11.2AYY34 pKa = 10.57DD35 pKa = 3.56AATNYY40 pKa = 9.65VSPPATDD47 pKa = 3.69YY48 pKa = 11.3GSSSATTPNGVVNQDD63 pKa = 3.4FAAVNQPLTTSFPQYY78 pKa = 10.92GYY80 pKa = 10.93DD81 pKa = 3.17AATNYY86 pKa = 9.69VSPPVTDD93 pKa = 3.95YY94 pKa = 11.52GSSSATATPNDD105 pKa = 3.73VVNQDD110 pKa = 3.63FAALNQSSTSSFPQYY125 pKa = 11.22AYY127 pKa = 9.12DD128 pKa = 3.44TATNYY133 pKa = 9.68VSPPTNNYY141 pKa = 9.94ASNDD145 pKa = 3.38TSGYY149 pKa = 9.49SPGYY153 pKa = 8.82DD154 pKa = 2.67TSTYY158 pKa = 10.35ASNYY162 pKa = 9.65NSGSSYY168 pKa = 10.97DD169 pKa = 3.29YY170 pKa = 10.88SYY172 pKa = 11.73NYY174 pKa = 10.05DD175 pKa = 3.22YY176 pKa = 11.11SYY178 pKa = 11.24SYY180 pKa = 11.73SDD182 pKa = 3.62FGGGDD187 pKa = 3.26FGFAPVVLDD196 pKa = 4.18LTGQGINITQLSQSNTFFDD215 pKa = 3.74ATGSGVQNQMAWAGAGNGVLFFDD238 pKa = 4.25PTGTGQLTQANQVIFTDD255 pKa = 3.84WDD257 pKa = 3.85PSAKK261 pKa = 9.94SDD263 pKa = 3.55MQALLDD269 pKa = 4.26VFDD272 pKa = 4.72TNHH275 pKa = 7.17DD276 pKa = 3.67GALNAGDD283 pKa = 4.29ANFSKK288 pKa = 10.46FYY290 pKa = 11.49VMVTNADD297 pKa = 3.45GTQTARR303 pKa = 11.84SLASLGITSINLVEE317 pKa = 5.37DD318 pKa = 3.98ATQVALPDD326 pKa = 3.93GSSIDD331 pKa = 3.65GQTSYY336 pKa = 8.03TTSGGTSGTVATVTFATSANGYY358 pKa = 9.54VVTTTTGTNADD369 pKa = 3.63GSVTIATSEE378 pKa = 4.31TNSDD382 pKa = 2.77GSLDD386 pKa = 3.45HH387 pKa = 7.19KK388 pKa = 10.92RR389 pKa = 11.84ILNTLVSSSTTSGVATTTTNRR410 pKa = 11.84EE411 pKa = 3.81LTTVNNGGVVLTLQTDD427 pKa = 4.47SISAASNGATTEE439 pKa = 3.86VLTNYY444 pKa = 10.29SAGTITSTGEE454 pKa = 3.65LTSAGTAGSEE464 pKa = 4.31KK465 pKa = 10.82LNSTTTATTVTSGGKK480 pKa = 9.57VVTIQRR486 pKa = 11.84DD487 pKa = 3.58LLGGGWTTQQEE498 pKa = 4.48VDD500 pKa = 3.6TTPSSGAATYY510 pKa = 10.34VVSDD514 pKa = 4.1LNFDD518 pKa = 3.75GTASHH523 pKa = 5.84VTSSTVTSGGLVRR536 pKa = 11.84TTIGLIDD543 pKa = 4.56GNSAMATTSVDD554 pKa = 2.93ATVIGSGGVRR564 pKa = 11.84TEE566 pKa = 4.49TITTSSGTTVTSLVQAVTSTTSNTVTRR593 pKa = 11.84TTSSYY598 pKa = 9.94LTDD601 pKa = 3.4GTTLDD606 pKa = 3.88RR607 pKa = 11.84VSVAQTVTTSSGSVTTQTDD626 pKa = 3.28RR627 pKa = 11.84SADD630 pKa = 3.59GTLLDD635 pKa = 4.8QVVTTQTPQSSGGLVTSAVTSALDD659 pKa = 3.19NGAFIGTGAQVTAISNAGGTATTTVTNYY687 pKa = 10.24SANGTLQSQRR697 pKa = 11.84ITSATLGSAARR708 pKa = 11.84TVTIYY713 pKa = 11.25GNGDD717 pKa = 3.08GRR719 pKa = 11.84ISQYY723 pKa = 9.1EE724 pKa = 4.26TVTVSSGTTTDD735 pKa = 3.17TVQLLNGDD743 pKa = 3.48GSIRR747 pKa = 11.84DD748 pKa = 3.63EE749 pKa = 4.64TVTSTASGGLSKK761 pKa = 9.59TLQIDD766 pKa = 3.52STGAGTAAAPVFDD779 pKa = 6.29HH780 pKa = 6.43ITTDD784 pKa = 3.36NTITSAGTSTEE795 pKa = 3.93TVTNYY800 pKa = 10.12GASTSYY806 pKa = 10.9KK807 pKa = 9.1IDD809 pKa = 3.32QSQTIVTSGGLQTIVSSAFTSSSLISGLWDD839 pKa = 3.43QVTTDD844 pKa = 4.21LTTISAGGALTEE856 pKa = 4.42TLTVTDD862 pKa = 4.88GVSHH866 pKa = 7.14ALKK869 pKa = 9.15TAQTNTSADD878 pKa = 3.3RR879 pKa = 11.84RR880 pKa = 11.84TRR882 pKa = 11.84TTTTTLGSTNLVKK895 pKa = 10.5QVEE898 pKa = 4.69TVTIANSGTVRR909 pKa = 11.84DD910 pKa = 3.83QVVSFDD916 pKa = 3.52QLGDD920 pKa = 3.6VLGATVTTSTADD932 pKa = 3.57GLVKK936 pKa = 9.84TVQKK940 pKa = 10.56DD941 pKa = 3.36IQGQSAAAFASGGLAFDD958 pKa = 3.83VTATDD963 pKa = 3.55TTVINADD970 pKa = 3.41GSRR973 pKa = 11.84TDD975 pKa = 3.59TVNTTAQNGALLSQTSVASSANGLSTTTTVNPYY1008 pKa = 8.63ATAHH1012 pKa = 5.8YY1013 pKa = 7.4ATRR1016 pKa = 11.84TARR1019 pKa = 11.84TTTLNGDD1026 pKa = 3.63GSSTVASATYY1036 pKa = 10.14SYY1038 pKa = 10.66NQVLIGSTQTVTAAGGLGTTTSQDD1062 pKa = 3.18LDD1064 pKa = 3.89GDD1066 pKa = 4.35GVTDD1070 pKa = 4.85LSTVDD1075 pKa = 4.36LVTINADD1082 pKa = 3.28GSRR1085 pKa = 11.84TEE1087 pKa = 4.06TVTDD1091 pKa = 3.74YY1092 pKa = 11.44TGTTAGAVRR1101 pKa = 11.84DD1102 pKa = 3.73VTTAHH1107 pKa = 6.35TGIIVAGAGLEE1118 pKa = 4.47SITTRR1123 pKa = 11.84QSLGSVPSYY1132 pKa = 10.22QVEE1135 pKa = 4.61TVLPAANGSVTDD1147 pKa = 3.63TTDD1150 pKa = 3.1YY1151 pKa = 10.52FASPGGALLLRR1162 pKa = 11.84TIATTSANGLVRR1174 pKa = 11.84TTATAVKK1181 pKa = 10.39GDD1183 pKa = 3.66TTTDD1187 pKa = 3.72FSTTDD1192 pKa = 4.1AITLNTDD1199 pKa = 3.52GSRR1202 pKa = 11.84TEE1204 pKa = 4.29TVSTSNKK1211 pKa = 8.81TGLLAEE1217 pKa = 4.39TVTVVSGNGLSRR1229 pKa = 11.84TTEE1232 pKa = 3.41VDD1234 pKa = 2.73ANGAVNGSGAAVFNRR1249 pKa = 11.84VITDD1253 pKa = 3.23NTVLNRR1259 pKa = 11.84TDD1261 pKa = 3.31GSRR1264 pKa = 11.84TEE1266 pKa = 4.33TVTTLNGVGTAITQSVTTVSADD1288 pKa = 3.49QQTTTTNRR1296 pKa = 11.84YY1297 pKa = 9.33LGEE1300 pKa = 4.33TGSITVVDD1308 pKa = 3.77QTEE1311 pKa = 4.23TRR1313 pKa = 11.84QTQTNGSVIDD1323 pKa = 3.86TVTSYY1328 pKa = 11.6NAFNSLLGSITRR1340 pKa = 11.84TTSGNGLSVQTVYY1353 pKa = 11.29KK1354 pKa = 10.22NAAGTATDD1362 pKa = 4.01TQSKK1366 pKa = 5.29TTTYY1370 pKa = 10.51NANGDD1375 pKa = 3.27GGYY1378 pKa = 10.72LVDD1381 pKa = 5.81AEE1383 pKa = 4.63DD1384 pKa = 4.57ADD1386 pKa = 4.38VVTGSTTLRR1395 pKa = 11.84SSVRR1399 pKa = 11.84TQVTGNGQQRR1409 pKa = 11.84TTTMTLSGAVAAQLAASSSVTTQDD1433 pKa = 3.0VVVIDD1438 pKa = 4.51DD1439 pKa = 4.17SGNTTEE1445 pKa = 4.56TTTDD1449 pKa = 3.31TLGSATVPTDD1459 pKa = 3.02TTVVVTSANGLSTTSSTAIGNGTPSIVEE1487 pKa = 4.15TKK1489 pKa = 10.46SINLDD1494 pKa = 3.27GSKK1497 pKa = 10.7SDD1499 pKa = 2.94TTTYY1503 pKa = 10.7YY1504 pKa = 11.22NSFAPSVIVAQTVVNTSFDD1523 pKa = 3.37GRR1525 pKa = 11.84TVSATTYY1532 pKa = 10.94SDD1534 pKa = 4.14FDD1536 pKa = 3.54GTQYY1540 pKa = 11.64NAVTDD1545 pKa = 3.98TFVANADD1552 pKa = 3.44NTTTEE1557 pKa = 4.16TRR1559 pKa = 11.84SGSGSFGAGAFGQIVTTLTNADD1581 pKa = 3.34ASKK1584 pKa = 8.07TTTTLNYY1591 pKa = 10.2DD1592 pKa = 3.49ATNLLIGQTVASVSGNGLAKK1612 pKa = 10.5SFVYY1616 pKa = 9.22DD1617 pKa = 3.33TTGQEE1622 pKa = 4.15SQAGLNAAASAILTGASVPASLLATDD1648 pKa = 5.42IIASDD1653 pKa = 3.43ITTLNPDD1660 pKa = 3.49GSQTEE1665 pKa = 4.45IIKK1668 pKa = 8.82TAYY1671 pKa = 10.08GNSFSNLRR1679 pKa = 11.84SQTITTTSANGLVTVTRR1696 pKa = 11.84IDD1698 pKa = 3.49NNGNGIFNQVDD1709 pKa = 3.92TTTVAPDD1716 pKa = 3.51GSKK1719 pKa = 9.25TVVCAYY1725 pKa = 10.64YY1726 pKa = 11.08GDD1728 pKa = 4.0TAATASTLLGTNTYY1742 pKa = 8.53TVSANGLVTTLATSTGVTDD1761 pKa = 3.64TTVTFANANGSYY1773 pKa = 8.72QWSRR1777 pKa = 11.84AVAANSPAVTNAAMPHH1793 pKa = 5.9NEE1795 pKa = 4.27SASHH1799 pKa = 6.59VIDD1802 pKa = 4.37ANGVDD1807 pKa = 3.76TWTLNDD1813 pKa = 4.88GYY1815 pKa = 10.72NLPHH1819 pKa = 7.44TITIDD1824 pKa = 3.37LASEE1828 pKa = 4.06KK1829 pKa = 10.21RR1830 pKa = 11.84DD1831 pKa = 3.33TAIANEE1837 pKa = 4.13IFQTLLGRR1845 pKa = 11.84TMDD1848 pKa = 3.8NAEE1851 pKa = 4.22SQSLLNFINNGTLDD1865 pKa = 3.55RR1866 pKa = 11.84YY1867 pKa = 10.76GLALGILQTAGYY1879 pKa = 8.77LSNYY1883 pKa = 8.68AVPGPTAGTYY1893 pKa = 9.66YY1894 pKa = 10.87YY1895 pKa = 11.08YY1896 pKa = 11.3GFDD1899 pKa = 3.12VTAAFQNAFGRR1910 pKa = 11.84PPTAEE1915 pKa = 3.66EE1916 pKa = 3.95MATFGQYY1923 pKa = 7.69MHH1925 pKa = 7.64DD1926 pKa = 4.08AAPSAQEE1933 pKa = 3.91LANLAVAVAQYY1944 pKa = 9.54ATDD1947 pKa = 3.52QGAKK1951 pKa = 9.81NNRR1954 pKa = 11.84TAVDD1958 pKa = 3.91PNQDD1962 pKa = 3.64LVTTAPQWISPAGSAVAITAAGTYY1986 pKa = 8.53TYY1988 pKa = 10.63SGQWLEE1994 pKa = 4.31DD1995 pKa = 3.64GNRR1998 pKa = 11.84VAGVGGVALTINGNNDD2014 pKa = 3.83AILALDD2020 pKa = 4.16GSVLTLNGYY2029 pKa = 9.37NNSVDD2034 pKa = 3.63IPGVTAWVNASNTAITIEE2052 pKa = 4.05DD2053 pKa = 3.51SGIGVVSGNNNQIAQVGATEE2073 pKa = 5.45LIVSSGTGNQIYY2085 pKa = 10.45VGAGALLGGFAYY2097 pKa = 7.05TTAYY2101 pKa = 9.5PITEE2105 pKa = 4.4ASNATITIGAGVGTFDD2121 pKa = 4.6NAAQVFGDD2129 pKa = 3.53NDD2131 pKa = 3.18IVYY2134 pKa = 9.44VDD2136 pKa = 3.38NGAYY2140 pKa = 10.16VSVVGNNDD2148 pKa = 3.96RR2149 pKa = 11.84IDD2151 pKa = 3.89VIGSGTAFVYY2161 pKa = 8.56GTASGSQISYY2171 pKa = 9.66GGVIEE2176 pKa = 4.35VVSGNTVNNTTVSSGGVLALYY2197 pKa = 10.52GGATASGTTLLAGGLLEE2214 pKa = 4.86IGSGYY2219 pKa = 6.81TQSGFSVGSGAFLQVDD2235 pKa = 3.56SGGAAVGTVVASGGTMILVGGEE2257 pKa = 4.08SDD2259 pKa = 3.43TGTTYY2264 pKa = 11.27AAGSFKK2270 pKa = 10.49EE2271 pKa = 4.06IIGGYY2276 pKa = 8.83VLGAYY2281 pKa = 7.29TVNNGVTLEE2290 pKa = 4.25VASGATVASAAVAAGNAAAVYY2311 pKa = 10.3AGGVVSAVTVSSGGSFTDD2329 pKa = 3.35YY2330 pKa = 10.98GSVYY2334 pKa = 9.58GANVALGASVTVGWGGLLDD2353 pKa = 3.88VMTGQSVSGVVVQNGGVLVVGSGGSASGTVVTSGGLLEE2391 pKa = 4.59LLGGATAVGTVLQQGAILDD2410 pKa = 3.91VGSGFTEE2417 pKa = 3.73TGYY2420 pKa = 10.44IVGSGMILAVEE2431 pKa = 4.29AGGAASGTVVQSGGTLEE2448 pKa = 4.17LAGGSWTNTTISAGGTLYY2466 pKa = 9.82ATSGASLSNYY2476 pKa = 6.96TVSSGVTLEE2485 pKa = 4.18VGAGAAVSNVTVSSGGEE2502 pKa = 3.49LAIYY2506 pKa = 9.35GGSVTGVVTAGGSVVVNNTGSATASTGQTVSGLTVSSGGVLTVAAGATARR2556 pKa = 11.84NIAVASGGQLTNFGTTSGAVVSSGGAATVSAGSLSILAGQTAWNVSVNSGANLVVSSGGTADD2618 pKa = 3.53NASIASGGHH2627 pKa = 5.26LVVNGTVVGATISNGGAVVSSGGVLEE2653 pKa = 5.99LDD2655 pKa = 3.66GQSANGVTVSSGGALIVDD2673 pKa = 5.13FSASASNVTVASGGSLSVVGYY2694 pKa = 10.41NSNLVGASIATGASAVVSGFAQLRR2718 pKa = 11.84VQSGQTVNGVTVAADD2733 pKa = 3.19GLLYY2737 pKa = 9.59VTSGGRR2743 pKa = 11.84ASGTVVSSGGTLEE2756 pKa = 4.15VFAGGVASATTVLNGGTYY2774 pKa = 10.08EE2775 pKa = 4.14VGSGLTQTGYY2785 pKa = 10.91VVASGTILEE2794 pKa = 4.43VASGGVVSGTSVSSGGTLEE2813 pKa = 4.33LLGGAAANGTTYY2825 pKa = 11.39ASGSFLEE2832 pKa = 4.63IVSGTTLSSYY2842 pKa = 8.3VTSANVTLEE2851 pKa = 3.62IGAGGTVNGATVNGSTALAVLYY2873 pKa = 10.87GGTLNNATVNSGGSLVVDD2891 pKa = 3.98GTVNGATVAAGGSVTVGAWGKK2912 pKa = 10.64LNVSSGQTVRR2922 pKa = 11.84SVSVTSGGSLSIAAGATASGTVVSNAGQIVVRR2954 pKa = 11.84GTASGSTISSGGAEE2968 pKa = 4.22VVTSGGTLSSAVVAGGMLEE2987 pKa = 4.09VMSGGSASGITLSSGGTLKK3006 pKa = 10.87LDD3008 pKa = 3.46TSVSYY3013 pKa = 10.78GATISGFGLPDD3024 pKa = 3.79HH3025 pKa = 7.33LDD3027 pKa = 3.51LADD3030 pKa = 3.39ISFGSSTTLSFAEE3043 pKa = 4.26AANNQSGTLTVSDD3056 pKa = 4.69GVHH3059 pKa = 5.64TAHH3062 pKa = 7.19LVLAGQYY3069 pKa = 5.58TTSRR3073 pKa = 11.84FTMASDD3079 pKa = 3.83GQGGTLVSDD3088 pKa = 4.89PPAEE3092 pKa = 3.82PAIASDD3098 pKa = 4.02EE3099 pKa = 4.17QHH3101 pKa = 6.89SSDD3104 pKa = 5.26AATQDD3109 pKa = 4.11AFVDD3113 pKa = 4.29LQKK3116 pKa = 11.49LLGFTDD3122 pKa = 3.48DD3123 pKa = 3.45WGSFIHH3129 pKa = 6.95VVDD3132 pKa = 3.62NWRR3135 pKa = 11.84QSDD3138 pKa = 3.96VPANVLPGVSTGNMEE3153 pKa = 3.91TDD3155 pKa = 3.29AQLSRR3160 pKa = 11.84LIDD3163 pKa = 3.45AMASFSVDD3171 pKa = 2.71DD3172 pKa = 4.39RR3173 pKa = 11.84GFVSAALTQEE3183 pKa = 4.05ASHH3186 pKa = 6.49DD3187 pKa = 3.78QRR3189 pKa = 11.84TPAQIAASWNGG3200 pKa = 3.06

Molecular weight:
318.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A4YLD7|A4YLD7_BRASO Putative Thiamine-phosphate diphosphorylase OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=BRADO0790 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84LATTGGRR28 pKa = 11.84KK29 pKa = 9.06VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight:
5.12 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6701

0

6701

2117499

20

5265

316.0

34.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.858 ± 0.048

0.859 ± 0.009

5.619 ± 0.022

5.183 ± 0.031

3.619 ± 0.021

8.34 ± 0.038

2.027 ± 0.017

5.248 ± 0.024

3.42 ± 0.028

9.997 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.422 ± 0.014

2.624 ± 0.021

5.323 ± 0.03

3.285 ± 0.015

7.088 ± 0.04

5.651 ± 0.03

5.467 ± 0.04

7.447 ± 0.025

1.326 ± 0.014

2.197 ± 0.015

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski