Klebsiella phage K1-ULIP33

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; unclassified Molineuxvirinae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P6D9C1|A0A4P6D9C1_9CAUD HNH homing endonuclease OS=Klebsiella phage K1-ULIP33 OX=2307015 GN=D3A55_0017 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.26RR3 pKa = 11.84NANAYY8 pKa = 10.37YY9 pKa = 10.1EE10 pKa = 4.32LQAQALEE17 pKa = 4.05LLKK20 pKa = 10.82EE21 pKa = 4.66RR22 pKa = 11.84IQYY25 pKa = 11.24DD26 pKa = 4.41DD27 pKa = 3.9ICDD30 pKa = 3.6SSDD33 pKa = 3.27ASDD36 pKa = 5.11AIHH39 pKa = 7.21EE40 pKa = 4.47IADD43 pKa = 3.9DD44 pKa = 3.97VTPHH48 pKa = 6.52YY49 pKa = 9.62YY50 pKa = 10.36HH51 pKa = 7.71EE52 pKa = 5.0IFTVMAADD60 pKa = 5.77GIDD63 pKa = 3.4TDD65 pKa = 5.26FEE67 pKa = 4.99DD68 pKa = 4.89SGLIPDD74 pKa = 4.6TKK76 pKa = 10.6DD77 pKa = 3.03VSQICQARR85 pKa = 11.84IYY87 pKa = 9.56EE88 pKa = 4.06QLTIDD93 pKa = 4.42MYY95 pKa = 11.85AEE97 pKa = 4.05IDD99 pKa = 3.93SLVEE103 pKa = 4.08EE104 pKa = 4.59YY105 pKa = 10.95VDD107 pKa = 4.5SIEE110 pKa = 5.2EE111 pKa = 3.91EE112 pKa = 4.27DD113 pKa = 4.03EE114 pKa = 4.12EE115 pKa = 4.68

Molecular weight: 13.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6DIN1|A0A4P6DIN1_9CAUD DNA primase/helicase OS=Klebsiella phage K1-ULIP33 OX=2307015 GN=D3A55_0013 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.14IKK4 pKa = 10.42RR5 pKa = 11.84VVKK8 pKa = 9.53NARR11 pKa = 11.84NAKK14 pKa = 10.19CYY16 pKa = 8.85GCSWRR21 pKa = 11.84KK22 pKa = 8.83FKK24 pKa = 11.04KK25 pKa = 9.99LVRR28 pKa = 11.84SSPDD32 pKa = 3.21EE33 pKa = 4.47NISRR37 pKa = 11.84IPARR41 pKa = 11.84KK42 pKa = 9.13LKK44 pKa = 10.39YY45 pKa = 9.52LWNSIKK51 pKa = 10.83

Molecular weight: 6.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13435

38

1264

248.8

27.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.52 ± 0.582

1.057 ± 0.169

6.17 ± 0.208

7.272 ± 0.335

3.714 ± 0.162

7.644 ± 0.308

2.188 ± 0.222

5.158 ± 0.224

6.424 ± 0.302

7.57 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.23 ± 0.161

4.079 ± 0.205

3.469 ± 0.18

4.31 ± 0.341

5.716 ± 0.262

5.62 ± 0.297

5.262 ± 0.301

6.982 ± 0.262

1.466 ± 0.144

3.148 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski