Mycobacterium phage Tweety

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; Mycobacterium virus Tweety

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5YK11|A5YK11_9CAUD Integrase OS=Mycobacterium phage Tweety OX=439809 GN=43 PE=3 SV=1
MM1 pKa = 7.85CGGCEE6 pKa = 4.05VKK8 pKa = 10.61SDD10 pKa = 3.51DD11 pKa = 3.64VRR13 pKa = 11.84YY14 pKa = 9.15EE15 pKa = 3.5VHH17 pKa = 6.52YY18 pKa = 10.88GDD20 pKa = 3.57KK21 pKa = 10.7TEE23 pKa = 4.67VFTSPYY29 pKa = 8.61ITQLWSWLGNVMEE42 pKa = 5.69PGDD45 pKa = 3.91TLHH48 pKa = 6.76WLSGGDD54 pKa = 3.7DD55 pKa = 3.31

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5YK67|A5YK67_9CAUD Uncharacterized protein OS=Mycobacterium phage Tweety OX=439809 GN=99 PE=4 SV=1
MM1 pKa = 7.31RR2 pKa = 11.84RR3 pKa = 11.84AARR6 pKa = 11.84RR7 pKa = 11.84LIATSRR13 pKa = 11.84RR14 pKa = 11.84LDD16 pKa = 3.51PPKK19 pKa = 10.97DD20 pKa = 3.42EE21 pKa = 4.21TRR23 pKa = 11.84LYY25 pKa = 10.62AGNITPEE32 pKa = 4.06PWEE35 pKa = 4.23HH36 pKa = 6.76LDD38 pKa = 3.83LYY40 pKa = 10.63RR41 pKa = 11.84PPSLLTRR48 pKa = 11.84IWWCIRR54 pKa = 11.84GG55 pKa = 3.61

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

18608

30

1176

170.7

18.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.151 ± 0.582

1.182 ± 0.159

6.701 ± 0.194

5.842 ± 0.292

2.821 ± 0.205

8.975 ± 0.591

2.241 ± 0.188

4.294 ± 0.187

3.208 ± 0.168

7.389 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.129

3.149 ± 0.18

5.911 ± 0.216

3.402 ± 0.177

6.82 ± 0.403

5.949 ± 0.259

6.712 ± 0.24

7.078 ± 0.269

2.451 ± 0.159

2.477 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski