Erythrobacter sp. NAP1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter; unclassified Erythrobacter

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3177 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3WGC5|A3WGC5_9SPHN Riboflavin synthase subunit alpha OS=Erythrobacter sp. NAP1 OX=237727 GN=NAP1_15628 PE=4 SV=1
MM1 pKa = 6.84NTLAKK6 pKa = 10.35SPSFAFAASVVAPLALVLSACGSEE30 pKa = 3.82PAVPVEE36 pKa = 3.93PPLYY40 pKa = 9.78GATIGGEE47 pKa = 3.84FDD49 pKa = 3.63LQNTSGEE56 pKa = 4.2AVKK59 pKa = 9.75WADD62 pKa = 3.2FNGQYY67 pKa = 10.22RR68 pKa = 11.84IVYY71 pKa = 9.47FGYY74 pKa = 10.32AYY76 pKa = 10.78CPDD79 pKa = 3.5ICPTDD84 pKa = 3.36VQRR87 pKa = 11.84TVQGLNQFTDD97 pKa = 3.73EE98 pKa = 4.07NPEE101 pKa = 4.13LGEE104 pKa = 3.81QVQPIFISVDD114 pKa = 3.3PDD116 pKa = 3.33RR117 pKa = 11.84DD118 pKa = 3.69TPEE121 pKa = 3.87VVEE124 pKa = 4.34EE125 pKa = 3.89FTNAFSEE132 pKa = 4.31RR133 pKa = 11.84LIGLTGSPDD142 pKa = 3.66QIADD146 pKa = 3.38AAKK149 pKa = 9.52TFGVYY154 pKa = 7.34YY155 pKa = 10.0TKK157 pKa = 10.71LDD159 pKa = 3.67SPSPDD164 pKa = 3.52TYY166 pKa = 11.65LMDD169 pKa = 3.95HH170 pKa = 6.49SRR172 pKa = 11.84TVLLFGPQGEE182 pKa = 4.43PLALLPADD190 pKa = 4.78LGADD194 pKa = 3.34AVAEE198 pKa = 4.03EE199 pKa = 4.28LGKK202 pKa = 9.83WVTT205 pKa = 3.61

Molecular weight: 22.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3WFH0|A3WFH0_9SPHN NAD-dependent protein deacylase OS=Erythrobacter sp. NAP1 OX=237727 GN=cobB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 7.42ATPGGRR28 pKa = 11.84KK29 pKa = 9.21VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.83RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03KK41 pKa = 10.62LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3177

0

3177

1002534

21

3529

315.6

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.262 ± 0.064

0.793 ± 0.014

6.198 ± 0.035

6.819 ± 0.044

3.825 ± 0.029

8.759 ± 0.058

1.864 ± 0.022

5.232 ± 0.03

3.188 ± 0.043

9.536 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.528 ± 0.021

2.805 ± 0.031

4.97 ± 0.032

3.163 ± 0.025

6.612 ± 0.047

5.697 ± 0.035

5.247 ± 0.033

6.937 ± 0.03

1.387 ± 0.02

2.179 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski