Mannheimia varigena USDA-ARS-USMARC-1296

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Mannheimia; Mannheimia varigena

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0Q962|W0Q962_9PAST Glucose-1-phosphate thymidylyltransferase OS=Mannheimia varigena USDA-ARS-USMARC-1296 OX=1433287 GN=X808_3050 PE=3 SV=1
MM1 pKa = 7.65NDD3 pKa = 2.81IQIPLIFTDD12 pKa = 3.67AAARR16 pKa = 11.84KK17 pKa = 9.3VKK19 pKa = 9.62TLIEE23 pKa = 4.56GEE25 pKa = 4.35DD26 pKa = 3.71NPNLRR31 pKa = 11.84LRR33 pKa = 11.84VYY35 pKa = 9.12ITGGGCSGFQYY46 pKa = 11.03GFTFDD51 pKa = 4.26DD52 pKa = 3.79QVNEE56 pKa = 3.92GDD58 pKa = 4.31LTIEE62 pKa = 4.05NQNVGLVVDD71 pKa = 4.64PMSLQYY77 pKa = 11.06LIGGTVDD84 pKa = 3.33YY85 pKa = 11.24TEE87 pKa = 5.04GLDD90 pKa = 3.4GSRR93 pKa = 11.84FVVTNPNASSTCGCGSSFSII113 pKa = 5.02

Molecular weight: 12.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0Q7P6|W0Q7P6_9PAST Cytochrome c-type biogenesis protein CcmF OS=Mannheimia varigena USDA-ARS-USMARC-1296 OX=1433287 GN=X808_3890 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2108

0

2108

667106

37

3855

316.5

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.602 ± 0.07

0.987 ± 0.017

4.909 ± 0.041

6.636 ± 0.056

4.496 ± 0.041

6.81 ± 0.07

1.962 ± 0.026

7.014 ± 0.055

6.321 ± 0.049

10.328 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.445 ± 0.032

4.855 ± 0.057

3.77 ± 0.036

4.482 ± 0.048

4.224 ± 0.046

5.871 ± 0.042

5.207 ± 0.056

6.841 ± 0.05

1.137 ± 0.023

3.104 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski