bacterium SCGC AG-212-C10

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177PYL2|A0A177PYL2_9BACT HTH merR-type domain-containing protein OS=bacterium SCGC AG-212-C10 OX=1799370 GN=AYO38_07020 PE=4 SV=1
MM1 pKa = 7.71LDD3 pKa = 3.35VFGTAVAISGNTIVVSGYY21 pKa = 11.16GEE23 pKa = 4.49DD24 pKa = 3.73SSTTGVNGDD33 pKa = 3.63QEE35 pKa = 5.13DD36 pKa = 4.05NSSATSFAAYY46 pKa = 10.05VFVRR50 pKa = 11.84NGATWVQQAYY60 pKa = 10.55LKK62 pKa = 10.87ASNNDD67 pKa = 3.12AGDD70 pKa = 3.56FFGYY74 pKa = 9.87SVAVSVDD81 pKa = 3.79TIVVGAINEE90 pKa = 4.07ASKK93 pKa = 9.99ATGVNHH99 pKa = 7.01EE100 pKa = 5.09EE101 pKa = 4.68GDD103 pKa = 3.83DD104 pKa = 3.81SAPEE108 pKa = 3.7AGAAYY113 pKa = 10.45VFVRR117 pKa = 11.84DD118 pKa = 3.7GTTWTQQAYY127 pKa = 10.31LKK129 pKa = 10.82ASNTDD134 pKa = 2.83AGDD137 pKa = 3.4RR138 pKa = 11.84FSYY141 pKa = 10.93VSVSGDD147 pKa = 3.59TLVVGADD154 pKa = 4.09LEE156 pKa = 4.42ASHH159 pKa = 7.22ASGKK163 pKa = 10.2NGNQADD169 pKa = 3.84NSAPQAGAAYY179 pKa = 10.24VFVRR183 pKa = 11.84SNQVWTQRR191 pKa = 11.84AYY193 pKa = 11.26LKK195 pKa = 10.31ATVAEE200 pKa = 4.33ANSFFGFSVAISGNAVLVGAYY221 pKa = 9.66GQDD224 pKa = 3.34VTVGGVGAAYY234 pKa = 9.79VYY236 pKa = 10.5PFEE239 pKa = 4.51QDD241 pKa = 3.33VFACDD246 pKa = 3.65GTLDD250 pKa = 3.95SADD253 pKa = 3.22ALAIFRR259 pKa = 11.84ALAGLTGAPTPTAPCTGDD277 pKa = 3.23YY278 pKa = 11.17DD279 pKa = 3.96EE280 pKa = 6.22SGDD283 pKa = 4.01VNLQDD288 pKa = 3.27VVAVLRR294 pKa = 11.84QLAGMTNLEE303 pKa = 4.25PQLQSLMFAPSPVAAGGSTTLSFTVTNADD332 pKa = 3.1

Molecular weight: 34.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177PZ98|A0A177PZ98_9BACT Glyoxalase OS=bacterium SCGC AG-212-C10 OX=1799370 GN=AYO38_05980 PE=4 SV=1
MM1 pKa = 7.14NLSVATRR8 pKa = 11.84IEE10 pKa = 4.31NNEE13 pKa = 4.09SPSLQRR19 pKa = 11.84FEE21 pKa = 4.5IPGQIVAMPAWKK33 pKa = 8.79RR34 pKa = 11.84TMDD37 pKa = 3.12IFGASVGLLMLSPLLVLVSAAVMLDD62 pKa = 3.23SRR64 pKa = 11.84GAPFFRR70 pKa = 11.84QRR72 pKa = 11.84RR73 pKa = 11.84IGRR76 pKa = 11.84GGMPFLCWKK85 pKa = 9.04FRR87 pKa = 11.84SMHH90 pKa = 5.53QGAEE94 pKa = 3.83RR95 pKa = 11.84LQAQLNAYY103 pKa = 9.75NEE105 pKa = 4.25ANGCIFKK112 pKa = 9.99MRR114 pKa = 11.84DD115 pKa = 3.07DD116 pKa = 3.81PRR118 pKa = 11.84RR119 pKa = 11.84TRR121 pKa = 11.84VGKK124 pKa = 8.35VLRR127 pKa = 11.84RR128 pKa = 11.84TSLDD132 pKa = 3.51EE133 pKa = 4.45LPQLINVLLGQMSLVGPRR151 pKa = 11.84PPVIPEE157 pKa = 3.81VVQYY161 pKa = 10.88QPDD164 pKa = 3.7EE165 pKa = 4.12LRR167 pKa = 11.84RR168 pKa = 11.84LAMVPGMTGLWQVSRR183 pKa = 11.84RR184 pKa = 11.84GAFEE188 pKa = 3.75FSEE191 pKa = 4.48MVALDD196 pKa = 3.46VEE198 pKa = 4.52YY199 pKa = 11.16GEE201 pKa = 5.11RR202 pKa = 11.84LTFRR206 pKa = 11.84RR207 pKa = 11.84DD208 pKa = 2.95IQILLRR214 pKa = 11.84TIPTVMFGRR223 pKa = 11.84GSYY226 pKa = 10.22

Molecular weight: 25.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2196

0

2196

665205

55

2017

302.9

32.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.062 ± 0.079

0.854 ± 0.017

5.753 ± 0.04

5.858 ± 0.06

3.612 ± 0.036

8.99 ± 0.059

2.002 ± 0.026

4.933 ± 0.037

2.583 ± 0.043

9.394 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.027

2.816 ± 0.045

5.388 ± 0.042

3.123 ± 0.028

6.886 ± 0.06

5.853 ± 0.047

5.914 ± 0.059

7.885 ± 0.043

1.448 ± 0.021

2.36 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski