Rhodococcus sp. MTM3W5.2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5842 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8YDD8|A0A1P8YDD8_9NOCA Peroxidase (Non-heme peroxidase) BpoB alpha/beta hydrolase family OS=Rhodococcus sp. MTM3W5.2 OX=1805827 GN=bpoB PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 4.2HH4 pKa = 7.33DD5 pKa = 4.19TTDD8 pKa = 3.76DD9 pKa = 4.79QPLQLDD15 pKa = 3.89GAFAIATDD23 pKa = 3.88TRR25 pKa = 11.84DD26 pKa = 3.57KK27 pKa = 11.12LHH29 pKa = 6.37SLSVRR34 pKa = 11.84TLAEE38 pKa = 4.11LLGSEE43 pKa = 5.0PDD45 pKa = 3.38TDD47 pKa = 3.72EE48 pKa = 6.36DD49 pKa = 4.16GDD51 pKa = 3.87IAIPVHH57 pKa = 6.25GFAVYY62 pKa = 8.47VTVADD67 pKa = 5.3DD68 pKa = 4.55GPQLHH73 pKa = 6.22VWSSIVTGITDD84 pKa = 3.79RR85 pKa = 11.84AQATTQLVALSEE97 pKa = 4.0EE98 pKa = 4.25WPRR101 pKa = 11.84LRR103 pKa = 11.84FALSEE108 pKa = 4.0EE109 pKa = 4.14HH110 pKa = 6.95LLVSTLVDD118 pKa = 4.05ADD120 pKa = 3.75PFAPQHH126 pKa = 6.09LLNLIDD132 pKa = 5.11EE133 pKa = 4.58IHH135 pKa = 7.29DD136 pKa = 3.91LTHH139 pKa = 7.74DD140 pKa = 4.41LDD142 pKa = 6.06DD143 pKa = 5.36DD144 pKa = 4.0FAAQFGGTIDD154 pKa = 4.9CDD156 pKa = 4.12ADD158 pKa = 3.87DD159 pKa = 5.12DD160 pKa = 5.35SYY162 pKa = 12.21AGDD165 pKa = 4.45CDD167 pKa = 4.16GGGCGGDD174 pKa = 4.17CACGDD179 pKa = 3.59NGEE182 pKa = 4.49HH183 pKa = 6.54VEE185 pKa = 4.14GDD187 pKa = 3.58LGITATTSSS196 pKa = 3.53

Molecular weight: 20.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8Y9H0|A0A1P8Y9H0_9NOCA Uncharacterized protein OS=Rhodococcus sp. MTM3W5.2 OX=1805827 GN=BTZ20_3543 PE=4 SV=1
MM1 pKa = 7.75PGPRR5 pKa = 11.84RR6 pKa = 11.84SSAPRR11 pKa = 11.84RR12 pKa = 11.84GRR14 pKa = 11.84APSRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84AGCPGRR27 pKa = 11.84TRR29 pKa = 11.84PGWSSSLRR37 pKa = 11.84RR38 pKa = 11.84PRR40 pKa = 11.84GRR42 pKa = 11.84PRR44 pKa = 11.84SCSGAARR51 pKa = 11.84RR52 pKa = 4.2

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5842

0

5842

1684694

37

8929

288.4

30.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.236 ± 0.043

0.807 ± 0.011

6.07 ± 0.031

5.528 ± 0.032

2.938 ± 0.019

9.313 ± 0.036

2.061 ± 0.015

4.05 ± 0.022

1.941 ± 0.023

10.035 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.999 ± 0.013

1.991 ± 0.018

5.778 ± 0.026

2.726 ± 0.018

7.51 ± 0.036

5.744 ± 0.024

6.144 ± 0.025

8.715 ± 0.037

1.485 ± 0.015

1.93 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski