Peptococcus niger

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Peptococcus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1G6SIS9|A0A1G6SIS9_PEPNI Thiamine diphosphokinase OS=Peptococcus niger OX=2741 GN=SAMN04489866_101329 PE=4 SV=1
MM1 pKa = 8.21DD2 pKa = 5.16KK3 pKa = 10.85PPLHH7 pKa = 5.2THH9 pKa = 6.34YY10 pKa = 10.6YY11 pKa = 9.42ALDD14 pKa = 3.64VAEE17 pKa = 4.99DD18 pKa = 3.78WQSFTYY24 pKa = 9.65ICKK27 pKa = 10.18EE28 pKa = 3.74NVMDD32 pKa = 5.58QMDD35 pKa = 4.64RR36 pKa = 11.84YY37 pKa = 10.93NFAAANWAYY46 pKa = 9.38TLEE49 pKa = 4.35DD50 pKa = 3.84LEE52 pKa = 4.79EE53 pKa = 4.16KK54 pKa = 10.07TFSILLFITGGDD66 pKa = 3.67EE67 pKa = 3.83ATVAQMILDD76 pKa = 4.23AEE78 pKa = 4.32AEE80 pKa = 4.31KK81 pKa = 11.22NDD83 pKa = 3.53

Molecular weight:
9.62 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1G6Z919|A0A1G6Z919_PEPNI Pyruvate orthophosphate dikinase OS=Peptococcus niger OX=2741 GN=SAMN04489866_11230 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.01QPKK8 pKa = 8.93RR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 8.0HH12 pKa = 4.33QRR14 pKa = 11.84VHH16 pKa = 6.02GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MLTKK25 pKa = 10.43NGRR28 pKa = 11.84NVLKK32 pKa = 10.49RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.49 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1844

0

1844

584678

30

3708

317.1

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.865 ± 0.09

1.133 ± 0.025

6.177 ± 0.054

6.248 ± 0.06

3.837 ± 0.046

7.465 ± 0.059

2.005 ± 0.025

6.269 ± 0.065

5.59 ± 0.083

10.139 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.035

3.621 ± 0.045

4.233 ± 0.042

3.636 ± 0.036

5.204 ± 0.054

5.216 ± 0.04

5.38 ± 0.059

6.901 ± 0.046

0.977 ± 0.018

3.413 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski