Streptococcus phage K13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060QNV1|A0A060QNV1_9CAUD Lactococcus phage M3 protein OS=Streptococcus phage K13 OX=1448274 PE=4 SV=1
MM1 pKa = 7.49FFQDD5 pKa = 3.76SEE7 pKa = 4.41IEE9 pKa = 4.12EE10 pKa = 4.55FEE12 pKa = 5.54LNDD15 pKa = 4.7ALMNDD20 pKa = 5.37CITAYY25 pKa = 9.68PDD27 pKa = 4.02EE28 pKa = 5.96IEE30 pKa = 5.04LMQSTGLKK38 pKa = 10.14DD39 pKa = 3.56KK40 pKa = 10.8NGKK43 pKa = 9.57EE44 pKa = 3.8IFEE47 pKa = 4.1GDD49 pKa = 2.94ILKK52 pKa = 10.86FNDD55 pKa = 3.03EE56 pKa = 4.11WNEE59 pKa = 3.91YY60 pKa = 8.78CHH62 pKa = 6.66EE63 pKa = 4.76GYY65 pKa = 10.91VDD67 pKa = 4.12GSVEE71 pKa = 3.79GVNYY75 pKa = 10.34VEE77 pKa = 4.4VVKK80 pKa = 11.26GEE82 pKa = 3.93ACFEE86 pKa = 4.26FGKK89 pKa = 8.67TRR91 pKa = 11.84YY92 pKa = 9.13PEE94 pKa = 4.12SSLFIYY100 pKa = 10.17MEE102 pKa = 4.56DD103 pKa = 3.22EE104 pKa = 4.15HH105 pKa = 8.96LSFAEE110 pKa = 4.08LVKK113 pKa = 11.17DD114 pKa = 3.37KK115 pKa = 11.45DD116 pKa = 3.72FGFEE120 pKa = 4.1IIDD123 pKa = 3.5NVYY126 pKa = 10.63EE127 pKa = 4.17NPEE130 pKa = 4.17LLEE133 pKa = 4.3VTHH136 pKa = 6.38EE137 pKa = 4.03

Molecular weight: 15.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060QSV5|A0A060QSV5_9CAUD Phage protein OS=Streptococcus phage K13 OX=1448274 PE=4 SV=1
MM1 pKa = 7.07FPKK4 pKa = 10.16WKK6 pKa = 10.05QLLDD10 pKa = 3.52KK11 pKa = 10.53KK12 pKa = 10.52KK13 pKa = 10.57SSRR16 pKa = 11.84IKK18 pKa = 9.91YY19 pKa = 9.29GYY21 pKa = 9.08HH22 pKa = 5.99HH23 pKa = 7.42VMDD26 pKa = 5.46LKK28 pKa = 11.01SKK30 pKa = 10.33CKK32 pKa = 10.5LYY34 pKa = 10.64GAKK37 pKa = 10.46LLIAVLL43 pKa = 3.94

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11117

38

2107

209.8

23.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.025 ± 0.681

0.612 ± 0.106

6.288 ± 0.297

8.402 ± 0.585

4.165 ± 0.306

6.117 ± 0.649

1.25 ± 0.146

6.198 ± 0.261

8.68 ± 0.552

8.384 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.264

5.442 ± 0.251

2.348 ± 0.212

4.363 ± 0.227

4.552 ± 0.395

6.135 ± 0.442

5.955 ± 0.405

6.585 ± 0.263

1.25 ± 0.141

3.886 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski