Methanosarcina spherical virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; unclassified Tectiviridae

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A290GPG8|A0A290GPG8_9VIRU ORF12 OS=Methanosarcina spherical virus OX=2035535 PE=4 SV=1
MM1 pKa = 7.34FNSITNKK8 pKa = 9.68IYY10 pKa = 11.12SLMTRR15 pKa = 11.84FNTFLNTPLWEE26 pKa = 5.74DD27 pKa = 4.57DD28 pKa = 3.49LTKK31 pKa = 10.94CNEE34 pKa = 3.93KK35 pKa = 10.44EE36 pKa = 4.07QEE38 pKa = 3.5ADD40 pKa = 3.57YY41 pKa = 11.62

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A290G8Q7|A0A290G8Q7_9VIRU Putative ATPase OS=Methanosarcina spherical virus OX=2035535 PE=4 SV=1
MM1 pKa = 7.72WSRR4 pKa = 11.84ILMVPLIIFTRR15 pKa = 11.84EE16 pKa = 3.42EE17 pKa = 3.6KK18 pKa = 9.85TALIFNEE25 pKa = 4.34KK26 pKa = 8.13TVKK29 pKa = 9.65TYY31 pKa = 10.86HH32 pKa = 6.58FDD34 pKa = 2.98QTIRR38 pKa = 11.84FRR40 pKa = 11.84VRR42 pKa = 11.84YY43 pKa = 8.81KK44 pKa = 11.03VKK46 pKa = 9.6TT47 pKa = 3.6

Molecular weight: 5.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

2817

33

359

128.0

14.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.07 ± 0.637

1.739 ± 0.344

4.757 ± 0.314

7.171 ± 0.822

4.224 ± 0.501

7.277 ± 0.868

0.887 ± 0.224

7.455 ± 0.519

8.129 ± 0.813

7.952 ± 0.671

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.241

5.112 ± 0.412

3.656 ± 0.296

3.301 ± 0.349

5.218 ± 0.584

7.455 ± 0.935

6.674 ± 0.704

5.467 ± 0.523

0.923 ± 0.184

4.295 ± 0.648
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski