Botrimarina hoheduenensis

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Lacipirellulaceae; Botrimarina

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3424 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C5W9S5|A0A5C5W9S5_9BACT PhoD-like phosphatase OS=Botrimarina hoheduenensis OX=2528000 GN=Pla111_12440 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 8.05PTRR5 pKa = 11.84TRR7 pKa = 11.84SAHH10 pKa = 5.15YY11 pKa = 9.86SSPRR15 pKa = 11.84GGGRR19 pKa = 11.84FACLLTRR26 pKa = 11.84TAGLLLAAASVGEE39 pKa = 4.21VTGLEE44 pKa = 4.11IVVPAYY50 pKa = 9.47FYY52 pKa = 10.34PSSSGSDD59 pKa = 2.99WDD61 pKa = 4.49RR62 pKa = 11.84LDD64 pKa = 3.3TAAAAVPITAIMNPFNGPGNSFNSDD89 pKa = 3.07YY90 pKa = 11.59GSATNSFTSAGGNLIGYY107 pKa = 8.25VYY109 pKa = 9.86SQYY112 pKa = 11.12GARR115 pKa = 11.84PLNVVLADD123 pKa = 3.35IDD125 pKa = 5.88RR126 pKa = 11.84YY127 pKa = 8.77AQWYY131 pKa = 7.61PVDD134 pKa = 4.78GIFVDD139 pKa = 4.26EE140 pKa = 5.19FSNSSDD146 pKa = 3.54PAVLDD151 pKa = 3.85YY152 pKa = 11.71YY153 pKa = 11.04NAIYY157 pKa = 10.21QYY159 pKa = 11.09VKK161 pKa = 10.66SIDD164 pKa = 3.85PAWEE168 pKa = 3.99VMGNPGTTTVEE179 pKa = 4.04NYY181 pKa = 7.37LTRR184 pKa = 11.84PAADD188 pKa = 3.82RR189 pKa = 11.84LMIWEE194 pKa = 4.52NFGATYY200 pKa = 7.8PTQSPPSWTANYY212 pKa = 8.15DD213 pKa = 3.0ASRR216 pKa = 11.84FVHH219 pKa = 6.75LLHH222 pKa = 6.55TLGSSSTATDD232 pKa = 3.8YY233 pKa = 11.84VDD235 pKa = 4.01LAVARR240 pKa = 11.84NVGGVYY246 pKa = 8.65FTDD249 pKa = 5.26DD250 pKa = 4.03EE251 pKa = 5.41LPNPWDD257 pKa = 3.83RR258 pKa = 11.84LPTYY262 pKa = 9.94WDD264 pKa = 3.12AFVAKK269 pKa = 10.26VEE271 pKa = 4.45SVNNQVFGSLEE282 pKa = 3.87QLSNPVAEE290 pKa = 4.37GAIMIDD296 pKa = 3.55SSRR299 pKa = 11.84SDD301 pKa = 2.98WSGLSAYY308 pKa = 10.64SPDD311 pKa = 3.57VDD313 pKa = 4.07DD314 pKa = 4.43TTSSSLDD321 pKa = 3.26IVEE324 pKa = 4.2VTLANDD330 pKa = 3.63PDD332 pKa = 3.91EE333 pKa = 5.12LFVRR337 pKa = 11.84LTLDD341 pKa = 3.68GSTTPLGNGHH351 pKa = 7.09RR352 pKa = 11.84LLIDD356 pKa = 3.09VDD358 pKa = 4.01GQRR361 pKa = 11.84GTGYY365 pKa = 10.82LGGSEE370 pKa = 4.04QFALGADD377 pKa = 3.74YY378 pKa = 11.14LILSDD383 pKa = 5.18RR384 pKa = 11.84LFAFQGLTQDD394 pKa = 3.41VFSWSFLADD403 pKa = 4.26LSTDD407 pKa = 3.36DD408 pKa = 4.52STPSDD413 pKa = 3.87IEE415 pKa = 4.0WAILLSDD422 pKa = 4.57LGSPDD427 pKa = 3.18VLNFFVEE434 pKa = 4.96TTSSAGNDD442 pKa = 3.75YY443 pKa = 10.97LPSRR447 pKa = 11.84AVDD450 pKa = 3.62GLAGGFYY457 pKa = 9.69RR458 pKa = 11.84YY459 pKa = 10.09KK460 pKa = 10.46IGTPPVAGDD469 pKa = 3.46YY470 pKa = 11.34DD471 pKa = 4.33GDD473 pKa = 4.23GDD475 pKa = 4.78VDD477 pKa = 3.63TDD479 pKa = 5.05DD480 pKa = 4.48YY481 pKa = 11.56EE482 pKa = 4.11VWKK485 pKa = 11.09ANFGGPNLAADD496 pKa = 4.28GNDD499 pKa = 3.94DD500 pKa = 4.19GVIDD504 pKa = 4.04AADD507 pKa = 3.47YY508 pKa = 8.3TVWRR512 pKa = 11.84DD513 pKa = 3.08AAMLAGGATIPEE525 pKa = 4.42PSLGSLLVAILTAITVAPHH544 pKa = 6.35RR545 pKa = 11.84RR546 pKa = 11.84QEE548 pKa = 3.76

Molecular weight: 58.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C5VTS3|A0A5C5VTS3_9BACT Mercuric reductase OS=Botrimarina hoheduenensis OX=2528000 GN=merA PE=3 SV=1
MM1 pKa = 7.57TKK3 pKa = 10.2PSSPGLNKK11 pKa = 9.47TLVMTRR17 pKa = 11.84LLVAKK22 pKa = 10.02FGMAAGEE29 pKa = 3.94PVRR32 pKa = 11.84TKK34 pKa = 10.25KK35 pKa = 10.05KK36 pKa = 10.27RR37 pKa = 11.84RR38 pKa = 11.84IAPKK42 pKa = 8.09PKK44 pKa = 8.94RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84PRR50 pKa = 11.84PKK52 pKa = 9.35PQLKK56 pKa = 9.63RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84APRR62 pKa = 11.84PKK64 pKa = 10.18RR65 pKa = 3.49

Molecular weight: 7.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3424

0

3424

1258017

30

6319

367.4

39.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.926 ± 0.056

1.048 ± 0.02

5.882 ± 0.043

6.128 ± 0.044

3.323 ± 0.024

8.441 ± 0.054

1.938 ± 0.021

4.168 ± 0.023

2.71 ± 0.037

10.356 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.851 ± 0.021

2.754 ± 0.029

5.643 ± 0.038

3.601 ± 0.024

7.257 ± 0.046

5.924 ± 0.034

5.76 ± 0.039

7.456 ± 0.031

1.476 ± 0.02

2.357 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski