Eubacterium sp. CAG:202

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1817 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6MTT3|R6MTT3_9FIRM Pseudouridine synthase OS=Eubacterium sp. CAG:202 OX=1262884 GN=BN531_01333 PE=3 SV=1
MM1 pKa = 7.19KK2 pKa = 8.24TTAKK6 pKa = 10.31FISASLATLLVSASAVTAFAAEE28 pKa = 4.38TKK30 pKa = 10.42DD31 pKa = 3.03ITVSLRR37 pKa = 11.84IEE39 pKa = 4.45GVDD42 pKa = 3.69SCVLYY47 pKa = 10.86DD48 pKa = 4.4NYY50 pKa = 10.55EE51 pKa = 3.93IPQGSTAADD60 pKa = 4.9LIQYY64 pKa = 10.16ADD66 pKa = 4.05KK67 pKa = 11.21LSDD70 pKa = 3.6DD71 pKa = 3.9VTVTGAEE78 pKa = 3.87NNYY81 pKa = 7.99ITDD84 pKa = 3.7VNGEE88 pKa = 4.13TAGKK92 pKa = 9.98FGGWDD97 pKa = 2.85GWQYY101 pKa = 11.15IVNSVSPNVGVGDD114 pKa = 3.86YY115 pKa = 9.69TLSDD119 pKa = 3.58NDD121 pKa = 4.23TVVLYY126 pKa = 11.13YY127 pKa = 11.13GDD129 pKa = 4.77FPCLLPQIDD138 pKa = 3.94TSALNSDD145 pKa = 3.78GKK147 pKa = 10.8ISFNAEE153 pKa = 3.61STTYY157 pKa = 11.09DD158 pKa = 3.5SDD160 pKa = 3.69WNPTVSTVAIADD172 pKa = 3.55MTVTFDD178 pKa = 2.87GHH180 pKa = 6.6TYY182 pKa = 7.78TTDD185 pKa = 3.23EE186 pKa = 4.69NGTISLSKK194 pKa = 10.92ADD196 pKa = 3.92FTSGEE201 pKa = 4.02HH202 pKa = 5.6SVQVEE207 pKa = 4.26KK208 pKa = 11.04KK209 pKa = 9.61NSNGAPAVLRR219 pKa = 11.84YY220 pKa = 10.11ADD222 pKa = 4.48DD223 pKa = 3.63FTVNIARR230 pKa = 11.84EE231 pKa = 3.78NTINVNLRR239 pKa = 11.84IEE241 pKa = 4.43GPEE244 pKa = 3.61ACYY247 pKa = 10.64LKK249 pKa = 10.24DD250 pKa = 3.18TFEE253 pKa = 4.09IAKK256 pKa = 10.04DD257 pKa = 3.76SNVGQLISYY266 pKa = 10.19ADD268 pKa = 3.64EE269 pKa = 4.12VSDD272 pKa = 4.09NIEE275 pKa = 4.08VVGADD280 pKa = 2.8AGYY283 pKa = 9.26ISEE286 pKa = 4.56VNGIAAGSFGGWDD299 pKa = 2.95GWYY302 pKa = 9.47YY303 pKa = 11.28AVNSVVPNVGVNDD316 pKa = 3.55YY317 pKa = 9.04TLNNNDD323 pKa = 3.77TVVLYY328 pKa = 10.73YY329 pKa = 10.87GEE331 pKa = 4.42YY332 pKa = 9.83PCYY335 pKa = 10.53LPIADD340 pKa = 4.18TSLLTSEE347 pKa = 4.71GKK349 pKa = 8.14ITFTATATFGDD360 pKa = 4.08AVLPIDD366 pKa = 3.96NMTVYY371 pKa = 10.59FDD373 pKa = 3.54GKK375 pKa = 10.92EE376 pKa = 3.79YY377 pKa = 8.12TTDD380 pKa = 3.34YY381 pKa = 10.87NGVVVIDD388 pKa = 4.06KK389 pKa = 10.63DD390 pKa = 3.58QLTFGSHH397 pKa = 5.15SLQVEE402 pKa = 4.41KK403 pKa = 10.83YY404 pKa = 8.86GYY406 pKa = 9.91SGAPAVLRR414 pKa = 11.84YY415 pKa = 10.1ADD417 pKa = 4.72DD418 pKa = 3.59YY419 pKa = 11.64TVFVDD424 pKa = 3.77TKK426 pKa = 10.57IVNDD430 pKa = 3.48VNLDD434 pKa = 3.72GNVDD438 pKa = 3.62INDD441 pKa = 3.21VTAIQTYY448 pKa = 9.89LSNYY452 pKa = 9.16NNISEE457 pKa = 4.33EE458 pKa = 3.97QVRR461 pKa = 11.84IADD464 pKa = 3.59VNKK467 pKa = 10.51DD468 pKa = 3.21GKK470 pKa = 11.27VDD472 pKa = 3.91VNDD475 pKa = 3.31VTALQTILSGEE486 pKa = 4.31TEE488 pKa = 3.91

Molecular weight: 52.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6N451|R6N451_9FIRM Uncharacterized protein OS=Eubacterium sp. CAG:202 OX=1262884 GN=BN531_00499 PE=3 SV=1
MM1 pKa = 8.11ADD3 pKa = 3.49RR4 pKa = 11.84PINRR8 pKa = 11.84GRR10 pKa = 11.84KK11 pKa = 8.61ARR13 pKa = 11.84KK14 pKa = 8.66KK15 pKa = 9.55VCGFCVDD22 pKa = 3.51KK23 pKa = 11.43VEE25 pKa = 5.09HH26 pKa = 6.88IDD28 pKa = 3.57YY29 pKa = 10.97KK30 pKa = 11.13DD31 pKa = 2.98IARR34 pKa = 11.84LRR36 pKa = 11.84RR37 pKa = 11.84YY38 pKa = 8.42MSEE41 pKa = 3.7RR42 pKa = 11.84GKK44 pKa = 10.14ILPRR48 pKa = 11.84RR49 pKa = 11.84VTGTCAKK56 pKa = 8.92HH57 pKa = 4.75QRR59 pKa = 11.84EE60 pKa = 3.87ITVAIKK66 pKa = 10.38RR67 pKa = 11.84ARR69 pKa = 11.84HH70 pKa = 5.37LALLPYY76 pKa = 9.54TADD79 pKa = 3.2

Molecular weight: 9.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1817

0

1817

547490

32

2146

301.3

33.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.351 ± 0.062

1.685 ± 0.024

5.99 ± 0.046

6.673 ± 0.058

4.412 ± 0.045

6.642 ± 0.05

1.426 ± 0.026

7.933 ± 0.058

7.727 ± 0.052

8.316 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.672 ± 0.027

5.606 ± 0.054

3.14 ± 0.034

2.633 ± 0.029

3.541 ± 0.046

6.51 ± 0.055

5.812 ± 0.055

7.032 ± 0.049

0.707 ± 0.018

4.192 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski