Alkalihalobacillus macyae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3973 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J6CP34|A0A0J6CP34_9BACI Phosphinothricin acetyltransferase OS=Alkalihalobacillus macyae OX=157733 GN=AB986_01335 PE=4 SV=1
MM1 pKa = 7.51TLTIPTAIEE10 pKa = 3.97CQAEE14 pKa = 4.17LTGTVTCDD22 pKa = 3.09GDD24 pKa = 3.87PVEE27 pKa = 4.14NAMVNFSSNPDD38 pKa = 2.83IVTFLPNPATTDD50 pKa = 2.93ASGNFTTTAMVPAGTDD66 pKa = 3.35PTAVTITAVTIVNAVEE82 pKa = 4.03ATTSEE87 pKa = 4.66DD88 pKa = 3.56STVSCPGEE96 pKa = 3.81EE97 pKa = 4.62CPCKK101 pKa = 10.76VRR103 pKa = 11.84IGTQDD108 pKa = 2.96SDD110 pKa = 3.77TTAMITTSDD119 pKa = 3.85GPSTIEE125 pKa = 3.72GQINISAVEE134 pKa = 4.42CFTAATMCNPAVDD147 pKa = 3.89NFQVAFSRR155 pKa = 11.84QGTTINFVMGRR166 pKa = 11.84RR167 pKa = 11.84TSLSCTNGTQTTIEE181 pKa = 4.28GTAKK185 pKa = 10.65AAGNVYY191 pKa = 10.11PPNTTFMVKK200 pKa = 9.08IDD202 pKa = 4.22LMISGPTATWTVMATDD218 pKa = 4.35GGSNTFSTTFMDD230 pKa = 4.58DD231 pKa = 3.29VTPEE235 pKa = 4.1TFIGDD240 pKa = 3.94CSSTPP245 pKa = 3.19

Molecular weight: 25.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J6CTE4|A0A0J6CTE4_9BACI Aldehyde dehydrogenase OS=Alkalihalobacillus macyae OX=157733 GN=AB986_10795 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84KK13 pKa = 8.49KK14 pKa = 8.81VHH16 pKa = 5.51GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.82VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3973

0

3973

1154936

27

3425

290.7

32.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.91 ± 0.042

0.665 ± 0.011

5.281 ± 0.031

7.748 ± 0.05

4.422 ± 0.036

7.086 ± 0.039

2.127 ± 0.019

7.382 ± 0.039

6.335 ± 0.035

9.799 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.819 ± 0.023

4.316 ± 0.031

3.649 ± 0.022

3.576 ± 0.023

4.182 ± 0.026

6.448 ± 0.028

5.52 ± 0.031

7.204 ± 0.032

1.039 ± 0.016

3.493 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski