Datura yellow vein nucleorhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7LI32|A0A0F7LI32_9RHAB Phosphoprotein OS=Datura yellow vein nucleorhabdovirus OX=195059 GN=P PE=4 SV=1
MM1 pKa = 7.6SDD3 pKa = 3.33KK4 pKa = 10.78VHH6 pKa = 6.76PRR8 pKa = 11.84YY9 pKa = 9.83QSLPKK14 pKa = 9.47TATSSEE20 pKa = 4.08VMASKK25 pKa = 10.58YY26 pKa = 10.09AAEE29 pKa = 4.06ISKK32 pKa = 9.03TGNTEE37 pKa = 3.76DD38 pKa = 4.32DD39 pKa = 4.2EE40 pKa = 5.82EE41 pKa = 4.28MMKK44 pKa = 9.54YY45 pKa = 8.08TEE47 pKa = 3.83EE48 pKa = 4.18WEE50 pKa = 3.99EE51 pKa = 3.79HH52 pKa = 5.0FRR54 pKa = 11.84RR55 pKa = 11.84EE56 pKa = 3.98GLTISNRR63 pKa = 11.84LIATLSEE70 pKa = 4.05VTLLCKK76 pKa = 10.45KK77 pKa = 10.24NNKK80 pKa = 9.25EE81 pKa = 3.77EE82 pKa = 4.16LAGKK86 pKa = 9.58LATTLVDD93 pKa = 3.53SFQSVIRR100 pKa = 11.84QHH102 pKa = 5.92TSLSNVVSHH111 pKa = 7.21LEE113 pKa = 3.81ALSEE117 pKa = 4.02NLFTGIDD124 pKa = 3.87EE125 pKa = 4.33IKK127 pKa = 10.87KK128 pKa = 10.61LGTDD132 pKa = 3.59LQKK135 pKa = 11.14ALPRR139 pKa = 11.84KK140 pKa = 9.62KK141 pKa = 10.15ISVSKK146 pKa = 10.55KK147 pKa = 7.56SSRR150 pKa = 11.84PIKK153 pKa = 9.64QAEE156 pKa = 4.32PIYY159 pKa = 10.49DD160 pKa = 3.71LSDD163 pKa = 3.44SPEE166 pKa = 4.05MMKK169 pKa = 10.24HH170 pKa = 4.9QPTTFSIPQEE180 pKa = 4.1QAPGTSSAPPPQTMEE195 pKa = 4.0HH196 pKa = 6.18EE197 pKa = 4.61TTKK200 pKa = 10.78EE201 pKa = 3.79PEE203 pKa = 4.01NNLEE207 pKa = 4.03SEE209 pKa = 4.28AMKK212 pKa = 10.3QLRR215 pKa = 11.84SSYY218 pKa = 9.86RR219 pKa = 11.84GYY221 pKa = 10.28YY222 pKa = 10.35SSDD225 pKa = 3.0EE226 pKa = 4.07FVAFDD231 pKa = 5.1IIKK234 pKa = 9.05QHH236 pKa = 6.12QIVNYY241 pKa = 9.34YY242 pKa = 9.26VEE244 pKa = 4.27NVLGFKK250 pKa = 10.22KK251 pKa = 10.56DD252 pKa = 3.75PEE254 pKa = 4.71DD255 pKa = 4.2KK256 pKa = 10.63DD257 pKa = 3.5PHH259 pKa = 6.54VLSMIFDD266 pKa = 4.13LVDD269 pKa = 3.31KK270 pKa = 10.66EE271 pKa = 4.44RR272 pKa = 11.84VLAVCKK278 pKa = 10.29KK279 pKa = 9.99IKK281 pKa = 9.78TGEE284 pKa = 4.02LDD286 pKa = 3.21EE287 pKa = 5.54ALITDD292 pKa = 3.84SVEE295 pKa = 4.46EE296 pKa = 3.88IVEE299 pKa = 4.75AINKK303 pKa = 8.56CGPAYY308 pKa = 10.26GEE310 pKa = 3.93RR311 pKa = 11.84TAEE314 pKa = 3.95EE315 pKa = 4.6VIVEE319 pKa = 4.09GRR321 pKa = 11.84SRR323 pKa = 11.84LVINN327 pKa = 4.73

Molecular weight: 36.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7LG26|A0A0F7LG26_9RHAB Glycoprotein OS=Datura yellow vein nucleorhabdovirus OX=195059 GN=G PE=4 SV=1
MM1 pKa = 7.52EE2 pKa = 5.56PASFSIKK9 pKa = 10.58GNVGLTFKK17 pKa = 10.74KK18 pKa = 10.51KK19 pKa = 9.55RR20 pKa = 11.84DD21 pKa = 3.57SMHH24 pKa = 7.26ALTLWDD30 pKa = 4.1IVTKK34 pKa = 8.86MWNMYY39 pKa = 8.2PEE41 pKa = 4.09RR42 pKa = 11.84VVVSFSTTRR51 pKa = 11.84NSADD55 pKa = 3.28KK56 pKa = 11.27SPVEE60 pKa = 4.38DD61 pKa = 4.17PLVIAAIQKK70 pKa = 8.62MVEE73 pKa = 4.23GCVKK77 pKa = 10.36SYY79 pKa = 11.0NIKK82 pKa = 10.26CSVGRR87 pKa = 11.84SVNLLLGDD95 pKa = 4.05CYY97 pKa = 10.93KK98 pKa = 10.93YY99 pKa = 10.91AIALGTSSKK108 pKa = 10.18DD109 pKa = 3.24TGHH112 pKa = 5.7EE113 pKa = 4.25TIILTLPFSMKK124 pKa = 9.39GCYY127 pKa = 9.26RR128 pKa = 11.84IHH130 pKa = 8.13AEE132 pKa = 4.43VSNHH136 pKa = 4.69MKK138 pKa = 10.32KK139 pKa = 10.58DD140 pKa = 3.66GVKK143 pKa = 8.39EE144 pKa = 3.9HH145 pKa = 6.35YY146 pKa = 9.29MLGIGLEE153 pKa = 4.13AYY155 pKa = 9.5IGDD158 pKa = 4.46MDD160 pKa = 4.23TEE162 pKa = 4.84GYY164 pKa = 9.69NAAIKK169 pKa = 10.24HH170 pKa = 6.44GICIYY175 pKa = 10.44PFMIEE180 pKa = 3.99HH181 pKa = 7.47PEE183 pKa = 4.06LFSKK187 pKa = 10.86SITSDD192 pKa = 3.11SEE194 pKa = 3.96MSSDD198 pKa = 3.16GGHH201 pKa = 5.39YY202 pKa = 10.2VAGPSAVMRR211 pKa = 11.84NKK213 pKa = 9.84KK214 pKa = 9.45RR215 pKa = 11.84RR216 pKa = 11.84KK217 pKa = 9.35QKK219 pKa = 9.94MLLKK223 pKa = 10.2SAKK226 pKa = 9.21PYY228 pKa = 10.39LSGRR232 pKa = 11.84ATSAKK237 pKa = 9.89KK238 pKa = 10.43VLNALRR244 pKa = 11.84LAGNKK249 pKa = 9.28IYY251 pKa = 10.73EE252 pKa = 4.58EE253 pKa = 4.46YY254 pKa = 7.92TTTDD258 pKa = 2.74GDD260 pKa = 3.73EE261 pKa = 4.41AQQQGNNTPSSQEE274 pKa = 3.61EE275 pKa = 4.17KK276 pKa = 11.07APATVPTKK284 pKa = 10.85

Molecular weight: 31.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4118

284

2106

686.3

77.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.78 ± 0.969

2.064 ± 0.235

5.585 ± 0.515

6.241 ± 0.771

3.084 ± 0.315

5.585 ± 0.406

2.695 ± 0.191

6.848 ± 0.308

6.605 ± 0.45

8.791 ± 0.787

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.181 ± 0.2

4.687 ± 0.142

4.177 ± 0.451

2.841 ± 0.159

5.148 ± 0.446

8.451 ± 0.163

6.581 ± 0.48

6.217 ± 0.335

1.36 ± 0.197

4.08 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski