Amino acid dipepetide frequency for Brettanomyces bruxellensis AWRI1499

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.008AlaAla: 5.008 ± 0.08	0.771AlaCys: 0.771 ± 0.023	3.527AlaAsp: 3.527 ± 0.049	3.982AlaGlu: 3.982 ± 0.059	2.415AlaPhe: 2.415 ± 0.038	3.775AlaGly: 3.775 ± 0.06	1.121AlaHis: 1.121 ± 0.025	3.755AlaIle: 3.755 ± 0.051	4.341AlaLys: 4.341 ± 0.051	5.657AlaLeu: 5.657 ± 0.058	1.307AlaMet: 1.307 ± 0.03	2.86AlaAsn: 2.86 ± 0.05	2.31AlaPro: 2.31 ± 0.041	2.152AlaGln: 2.152 ± 0.043	2.883AlaArg: 2.883 ± 0.045	5.471AlaSer: 5.471 ± 0.071	3.417AlaThr: 3.417 ± 0.045	3.841AlaVal: 3.841 ± 0.052	0.51AlaTrp: 0.51 ± 0.019	1.85AlaTyr: 1.85 ± 0.036	0.868AlaXaa: 0.868 ± 0.028
Cys	0.689CysAla: 0.689 ± 0.023	0.303CysCys: 0.303 ± 0.014	0.699CysAsp: 0.699 ± 0.02	0.613CysGlu: 0.613 ± 0.021	0.709CysPhe: 0.709 ± 0.022	0.945CysGly: 0.945 ± 0.027	0.297CysHis: 0.297 ± 0.012	0.833CysIle: 0.833 ± 0.024	0.699CysLys: 0.699 ± 0.021	1.279CysLeu: 1.279 ± 0.028	0.356CysMet: 0.356 ± 0.014	0.502CysAsn: 0.502 ± 0.017	0.525CysPro: 0.525 ± 0.02	0.374CysGln: 0.374 ± 0.015	0.576CysArg: 0.576 ± 0.02	1.058CysSer: 1.058 ± 0.027	0.651CysThr: 0.651 ± 0.024	0.786CysVal: 0.786 ± 0.021	0.195CysTrp: 0.195 ± 0.011	0.534CysTyr: 0.534 ± 0.018	0.162CysXaa: 0.162 ± 0.01
Asp	3.679AspAla: 3.679 ± 0.044	0.668AspCys: 0.668 ± 0.022	5.191AspAsp: 5.191 ± 0.102	5.211AspGlu: 5.211 ± 0.072	2.619AspPhe: 2.619 ± 0.038	3.483AspGly: 3.483 ± 0.057	1.071AspHis: 1.071 ± 0.023	3.822AspIle: 3.822 ± 0.047	3.819AspLys: 3.819 ± 0.055	5.381AspLeu: 5.381 ± 0.057	1.287AspMet: 1.287 ± 0.031	2.8AspAsn: 2.8 ± 0.042	2.654AspPro: 2.654 ± 0.04	1.874AspGln: 1.874 ± 0.029	2.436AspArg: 2.436 ± 0.048	5.086AspSer: 5.086 ± 0.055	2.927AspThr: 2.927 ± 0.045	3.722AspVal: 3.722 ± 0.045	0.639AspTrp: 0.639 ± 0.018	2.209AspTyr: 2.209 ± 0.036	1.156AspXaa: 1.156 ± 0.032
Glu	3.913GluAla: 3.913 ± 0.059	0.643GluCys: 0.643 ± 0.019	4.616GluAsp: 4.616 ± 0.065	6.054GluGlu: 6.054 ± 0.103	2.475GluPhe: 2.475 ± 0.038	3.02GluGly: 3.02 ± 0.047	1.206GluHis: 1.206 ± 0.025	3.971GluIle: 3.971 ± 0.052	5.794GluLys: 5.794 ± 0.092	5.805GluLeu: 5.805 ± 0.075	1.555GluMet: 1.555 ± 0.036	3.713GluAsn: 3.713 ± 0.049	1.986GluPro: 1.986 ± 0.041	2.64GluGln: 2.64 ± 0.042	3.097GluArg: 3.097 ± 0.048	4.966GluSer: 4.966 ± 0.067	3.303GluThr: 3.303 ± 0.047	3.49GluVal: 3.49 ± 0.058	0.565GluTrp: 0.565 ± 0.019	2.166GluTyr: 2.166 ± 0.034	1.241GluXaa: 1.241 ± 0.031
Phe	2.496PheAla: 2.496 ± 0.041	0.618PheCys: 0.618 ± 0.021	2.749PheAsp: 2.749 ± 0.038	2.518PheGlu: 2.518 ± 0.04	1.826PhePhe: 1.826 ± 0.032	2.984PheGly: 2.984 ± 0.057	0.834PheHis: 0.834 ± 0.02	2.349PheIle: 2.349 ± 0.043	2.854PheLys: 2.854 ± 0.048	3.55PheLeu: 3.55 ± 0.05	0.987PheMet: 0.987 ± 0.022	2.071PheAsn: 2.071 ± 0.039	1.607PhePro: 1.607 ± 0.033	1.512PheGln: 1.512 ± 0.035	1.883PheArg: 1.883 ± 0.03	3.432PheSer: 3.432 ± 0.053	2.166PheThr: 2.166 ± 0.035	2.644PheVal: 2.644 ± 0.039	0.516PheTrp: 0.516 ± 0.016	1.445PheTyr: 1.445 ± 0.03	0.503PheXaa: 0.503 ± 0.021
Gly	3.479GlyAla: 3.479 ± 0.06	0.884GlyCys: 0.884 ± 0.026	3.225GlyAsp: 3.225 ± 0.044	3.383GlyGlu: 3.383 ± 0.049	2.653GlyPhe: 2.653 ± 0.041	3.766GlyGly: 3.766 ± 0.071	1.265GlyHis: 1.265 ± 0.03	3.567GlyIle: 3.567 ± 0.046	4.205GlyLys: 4.205 ± 0.057	4.943GlyLeu: 4.943 ± 0.065	1.37GlyMet: 1.37 ± 0.028	2.876GlyAsn: 2.876 ± 0.049	1.803GlyPro: 1.803 ± 0.032	1.824GlyGln: 1.824 ± 0.038	2.83GlyArg: 2.83 ± 0.048	5.298GlySer: 5.298 ± 0.067	3.077GlyThr: 3.077 ± 0.046	3.494GlyVal: 3.494 ± 0.052	0.673GlyTrp: 0.673 ± 0.02	2.092GlyTyr: 2.092 ± 0.038	0.9GlyXaa: 0.9 ± 0.027
His	1.04HisAla: 1.04 ± 0.026	0.271HisCys: 0.271 ± 0.012	1.06HisAsp: 1.06 ± 0.025	1.092HisGlu: 1.092 ± 0.027	0.843HisPhe: 0.843 ± 0.023	1.177HisGly: 1.177 ± 0.03	0.667HisHis: 0.667 ± 0.034	1.201HisIle: 1.201 ± 0.024	1.234HisLys: 1.234 ± 0.028	1.864HisLeu: 1.864 ± 0.035	0.441HisMet: 0.441 ± 0.016	0.9HisAsn: 0.9 ± 0.024	1.097HisPro: 1.097 ± 0.027	0.747HisGln: 0.747 ± 0.022	1.184HisArg: 1.184 ± 0.028	1.709HisSer: 1.709 ± 0.032	0.996HisThr: 0.996 ± 0.023	1.123HisVal: 1.123 ± 0.027	0.232HisTrp: 0.232 ± 0.012	0.729HisTyr: 0.729 ± 0.021	0.34HisXaa: 0.34 ± 0.015
Ile	3.596IleAla: 3.596 ± 0.048	0.934IleCys: 0.934 ± 0.023	3.838IleAsp: 3.838 ± 0.055	3.564IleGlu: 3.564 ± 0.049	2.48IlePhe: 2.48 ± 0.045	3.371IleGly: 3.371 ± 0.051	1.223IleHis: 1.223 ± 0.028	3.319IleIle: 3.319 ± 0.053	3.795IleLys: 3.795 ± 0.048	5.291IleLeu: 5.291 ± 0.071	1.304IleMet: 1.304 ± 0.029	2.85IleAsn: 2.85 ± 0.042	3.129IlePro: 3.129 ± 0.046	2.192IleGln: 2.192 ± 0.041	2.909IleArg: 2.909 ± 0.037	5.216IleSer: 5.216 ± 0.07	3.098IleThr: 3.098 ± 0.046	3.521IleVal: 3.521 ± 0.052	0.638IleTrp: 0.638 ± 0.02	1.859IleTyr: 1.859 ± 0.032	0.759IleXaa: 0.759 ± 0.021
Lys	4.419LysAla: 4.419 ± 0.061	0.777LysCys: 0.777 ± 0.019	4.235LysAsp: 4.235 ± 0.059	5.365LysGlu: 5.365 ± 0.079	2.843LysPhe: 2.843 ± 0.041	3.308LysGly: 3.308 ± 0.048	1.502LysHis: 1.502 ± 0.033	4.232LysIle: 4.232 ± 0.055	6.415LysLys: 6.415 ± 0.092	6.728LysLeu: 6.728 ± 0.068	1.681LysMet: 1.681 ± 0.032	3.561LysAsn: 3.561 ± 0.049	2.816LysPro: 2.816 ± 0.047	2.947LysGln: 2.947 ± 0.046	3.922LysArg: 3.922 ± 0.056	5.265LysSer: 5.265 ± 0.072	3.532LysThr: 3.532 ± 0.05	4.088LysVal: 4.088 ± 0.052	0.701LysTrp: 0.701 ± 0.02	2.672LysTyr: 2.672 ± 0.046	1.32LysXaa: 1.32 ± 0.033
Leu	5.621LeuAla: 5.621 ± 0.062	1.244LeuCys: 1.244 ± 0.031	5.471LeuAsp: 5.471 ± 0.059	5.661LeuGlu: 5.661 ± 0.07	3.576LeuPhe: 3.576 ± 0.057	4.884LeuGly: 4.884 ± 0.058	1.829LeuHis: 1.829 ± 0.032	4.891LeuIle: 4.891 ± 0.066	6.863LeuLys: 6.863 ± 0.08	8.109LeuLeu: 8.109 ± 0.1	2.006LeuMet: 2.006 ± 0.036	4.46LeuAsn: 4.46 ± 0.056	4.073LeuPro: 4.073 ± 0.05	3.634LeuGln: 3.634 ± 0.048	4.547LeuArg: 4.547 ± 0.053	7.743LeuSer: 7.743 ± 0.078	4.71LeuThr: 4.71 ± 0.053	5.01LeuVal: 5.01 ± 0.059	0.831LeuTrp: 0.831 ± 0.022	2.837LeuTyr: 2.837 ± 0.041	1.109LeuXaa: 1.109 ± 0.028
Met	1.523MetAla: 1.523 ± 0.031	0.295MetCys: 0.295 ± 0.011	1.433MetAsp: 1.433 ± 0.031	1.372MetGlu: 1.372 ± 0.028	0.879MetPhe: 0.879 ± 0.023	1.296MetGly: 1.296 ± 0.028	0.41MetHis: 0.41 ± 0.015	1.336MetIle: 1.336 ± 0.029	1.678MetLys: 1.678 ± 0.033	2.052MetLeu: 2.052 ± 0.03	0.62MetMet: 0.62 ± 0.021	1.254MetAsn: 1.254 ± 0.03	0.981MetPro: 0.981 ± 0.024	0.866MetGln: 0.866 ± 0.025	1.034MetArg: 1.034 ± 0.021	2.021MetSer: 2.021 ± 0.033	1.213MetThr: 1.213 ± 0.025	1.233MetVal: 1.233 ± 0.03	0.177MetTrp: 0.177 ± 0.009	0.689MetTyr: 0.689 ± 0.023	0.284MetXaa: 0.284 ± 0.013
Asn	3.042AsnAla: 3.042 ± 0.044	0.609AsnCys: 0.609 ± 0.02	3.018AsnAsp: 3.018 ± 0.044	3.244AsnGlu: 3.244 ± 0.045	2.127AsnPhe: 2.127 ± 0.035	3.278AsnGly: 3.278 ± 0.054	0.834AsnHis: 0.834 ± 0.024	2.947AsnIle: 2.947 ± 0.049	3.135AsnLys: 3.135 ± 0.05	4.257AsnLeu: 4.257 ± 0.057	1.221AsnMet: 1.221 ± 0.027	2.573AsnAsn: 2.573 ± 0.071	2.143AsnPro: 2.143 ± 0.031	1.558AsnGln: 1.558 ± 0.036	2.075AsnArg: 2.075 ± 0.041	4.446AsnSer: 4.446 ± 0.06	2.587AsnThr: 2.587 ± 0.043	2.97AsnVal: 2.97 ± 0.049	0.521AsnTrp: 0.521 ± 0.02	1.717AsnTyr: 1.717 ± 0.034	1.039AsnXaa: 1.039 ± 0.029
Pro	2.643ProAla: 2.643 ± 0.048	0.346ProCys: 0.346 ± 0.017	2.515ProAsp: 2.515 ± 0.035	3.324ProGlu: 3.324 ± 0.049	1.765ProPhe: 1.765 ± 0.037	2.301ProGly: 2.301 ± 0.038	0.754ProHis: 0.754 ± 0.023	2.412ProIle: 2.412 ± 0.038	2.81ProLys: 2.81 ± 0.05	3.529ProLeu: 3.529 ± 0.045	0.814ProMet: 0.814 ± 0.023	1.881ProAsn: 1.881 ± 0.035	2.003ProPro: 2.003 ± 0.061	1.605ProGln: 1.605 ± 0.044	1.821ProArg: 1.821 ± 0.031	3.57ProSer: 3.57 ± 0.05	2.325ProThr: 2.325 ± 0.037	2.66ProVal: 2.66 ± 0.042	0.411ProTrp: 0.411 ± 0.016	1.358ProTyr: 1.358 ± 0.029	0.687ProXaa: 0.687 ± 0.022
Gln	2.158GlnAla: 2.158 ± 0.042	0.42GlnCys: 0.42 ± 0.015	1.76GlnAsp: 1.76 ± 0.032	2.254GlnGlu: 2.254 ± 0.045	1.564GlnPhe: 1.564 ± 0.03	1.782GlnGly: 1.782 ± 0.037	0.773GlnHis: 0.773 ± 0.024	2.384GlnIle: 2.384 ± 0.042	2.897GlnLys: 2.897 ± 0.047	3.638GlnLeu: 3.638 ± 0.046	0.972GlnMet: 0.972 ± 0.026	1.929GlnAsn: 1.929 ± 0.034	1.497GlnPro: 1.497 ± 0.039	2.884GlnGln: 2.884 ± 0.123	1.967GlnArg: 1.967 ± 0.035	2.73GlnSer: 2.73 ± 0.042	1.849GlnThr: 1.849 ± 0.037	1.931GlnVal: 1.931 ± 0.032	0.347GlnTrp: 0.347 ± 0.013	1.276GlnTyr: 1.276 ± 0.031	0.654GlnXaa: 0.654 ± 0.021
Arg	2.805ArgAla: 2.805 ± 0.042	0.632ArgCys: 0.632 ± 0.021	2.483ArgAsp: 2.483 ± 0.04	2.954ArgGlu: 2.954 ± 0.049	2.072ArgPhe: 2.072 ± 0.036	2.489ArgGly: 2.489 ± 0.043	1.153ArgHis: 1.153 ± 0.026	2.908ArgIle: 2.908 ± 0.043	4.131ArgLys: 4.131 ± 0.055	4.53ArgLeu: 4.53 ± 0.057	1.208ArgMet: 1.208 ± 0.024	2.447ArgAsn: 2.447 ± 0.038	1.834ArgPro: 1.834 ± 0.04	1.923ArgGln: 1.923 ± 0.038	3.509ArgArg: 3.509 ± 0.057	3.833ArgSer: 3.833 ± 0.053	2.365ArgThr: 2.365 ± 0.04	2.615ArgVal: 2.615 ± 0.036	0.497ArgTrp: 0.497 ± 0.016	1.72ArgTyr: 1.72 ± 0.034	0.687ArgXaa: 0.687 ± 0.022
Ser	5.222SerAla: 5.222 ± 0.075	0.974SerCys: 0.974 ± 0.025	5.074SerAsp: 5.074 ± 0.059	5.09SerGlu: 5.09 ± 0.059	3.528SerPhe: 3.528 ± 0.049	5.255SerGly: 5.255 ± 0.058	1.62SerHis: 1.62 ± 0.035	4.967SerIle: 4.967 ± 0.061	5.936SerLys: 5.936 ± 0.068	7.46SerLeu: 7.46 ± 0.079	1.816SerMet: 1.816 ± 0.034	4.289SerAsn: 4.289 ± 0.058	3.504SerPro: 3.504 ± 0.067	2.98SerGln: 2.98 ± 0.047	4.135SerArg: 4.135 ± 0.054	10.21SerSer: 10.21 ± 0.164	5.417SerThr: 5.417 ± 0.089	4.812SerVal: 4.812 ± 0.057	0.778SerTrp: 0.778 ± 0.023	2.463SerTyr: 2.463 ± 0.043	1.617SerXaa: 1.617 ± 0.045
Thr	3.444ThrAla: 3.444 ± 0.053	0.624ThrCys: 0.624 ± 0.019	2.981ThrAsp: 2.981 ± 0.041	3.103ThrGlu: 3.103 ± 0.043	2.3ThrPhe: 2.3 ± 0.036	3.412ThrGly: 3.412 ± 0.055	0.959ThrHis: 0.959 ± 0.025	3.247ThrIle: 3.247 ± 0.045	3.487ThrLys: 3.487 ± 0.044	4.678ThrLeu: 4.678 ± 0.049	1.035ThrMet: 1.035 ± 0.025	2.461ThrAsn: 2.461 ± 0.039	2.586ThrPro: 2.586 ± 0.04	1.653ThrGln: 1.653 ± 0.036	2.332ThrArg: 2.332 ± 0.04	5.048ThrSer: 5.048 ± 0.098	3.334ThrThr: 3.334 ± 0.087	3.327ThrVal: 3.327 ± 0.052	0.523ThrTrp: 0.523 ± 0.018	1.617ThrTyr: 1.617 ± 0.036	0.844ThrXaa: 0.844 ± 0.026
Val	3.74ValAla: 3.74 ± 0.056	0.86ValCys: 0.86 ± 0.025	3.937ValAsp: 3.937 ± 0.049	3.746ValGlu: 3.746 ± 0.065	2.353ValPhe: 2.353 ± 0.037	3.386ValGly: 3.386 ± 0.055	1.186ValHis: 1.186 ± 0.027	3.444ValIle: 3.444 ± 0.049	4.084ValLys: 4.084 ± 0.061	5.138ValLeu: 5.138 ± 0.062	1.257ValMet: 1.257 ± 0.028	2.716ValAsn: 2.716 ± 0.041	2.727ValPro: 2.727 ± 0.036	2.055ValGln: 2.055 ± 0.035	2.732ValArg: 2.732 ± 0.041	5.015ValSer: 5.015 ± 0.048	2.959ValThr: 2.959 ± 0.047	3.744ValVal: 3.744 ± 0.052	0.572ValTrp: 0.572 ± 0.017	1.858ValTyr: 1.858 ± 0.038	0.711ValXaa: 0.711 ± 0.02
Trp	0.521TrpAla: 0.521 ± 0.018	0.227TrpCys: 0.227 ± 0.012	0.627TrpAsp: 0.627 ± 0.022	0.502TrpGlu: 0.502 ± 0.019	0.446TrpPhe: 0.446 ± 0.017	0.586TrpGly: 0.586 ± 0.018	0.193TrpHis: 0.193 ± 0.011	0.647TrpIle: 0.647 ± 0.02	0.844TrpLys: 0.844 ± 0.022	0.872TrpLeu: 0.872 ± 0.024	0.279TrpMet: 0.279 ± 0.013	0.586TrpAsn: 0.586 ± 0.019	0.292TrpPro: 0.292 ± 0.014	0.348TrpGln: 0.348 ± 0.014	0.552TrpArg: 0.552 ± 0.017	0.764TrpSer: 0.764 ± 0.021	0.54TrpThr: 0.54 ± 0.02	0.53TrpVal: 0.53 ± 0.017	0.152TrpTrp: 0.152 ± 0.01	0.362TrpTyr: 0.362 ± 0.014	0.123TrpXaa: 0.123 ± 0.008
Tyr	1.91TyrAla: 1.91 ± 0.035	0.504TyrCys: 0.504 ± 0.018	2.196TyrAsp: 2.196 ± 0.042	1.995TyrGlu: 1.995 ± 0.04	1.508TyrPhe: 1.508 ± 0.034	2.159TyrGly: 2.159 ± 0.038	0.729TyrHis: 0.729 ± 0.022	1.883TyrIle: 1.883 ± 0.039	2.089TyrLys: 2.089 ± 0.038	3.195TyrLeu: 3.195 ± 0.047	0.783TyrMet: 0.783 ± 0.024	1.678TyrAsn: 1.678 ± 0.035	1.385TyrPro: 1.385 ± 0.031	1.22TyrGln: 1.22 ± 0.029	1.637TyrArg: 1.637 ± 0.033	2.6TyrSer: 2.6 ± 0.041	1.728TyrThr: 1.728 ± 0.035	1.883TyrVal: 1.883 ± 0.038	0.395TyrTrp: 0.395 ± 0.015	1.338TyrTyr: 1.338 ± 0.03	0.401TyrXaa: 0.401 ± 0.016
Xaa	0.873XaaAla: 0.873 ± 0.025	0.18XaaCys: 0.18 ± 0.011	1.101XaaAsp: 1.101 ± 0.028	1.19XaaGlu: 1.19 ± 0.038	0.563XaaPhe: 0.563 ± 0.019	0.952XaaGly: 0.952 ± 0.026	0.319XaaHis: 0.319 ± 0.015	0.773XaaIle: 0.773 ± 0.019	1.22XaaLys: 1.22 ± 0.035	1.153XaaLeu: 1.153 ± 0.029	0.344XaaMet: 0.344 ± 0.015	0.934XaaAsn: 0.934 ± 0.029	0.583XaaPro: 0.583 ± 0.021	0.679XaaGln: 0.679 ± 0.024	0.736XaaArg: 0.736 ± 0.025	1.665XaaSer: 1.665 ± 0.045	0.801XaaThr: 0.801 ± 0.024	0.794XaaVal: 0.794 ± 0.024	0.122XaaTrp: 0.122 ± 0.008	0.423XaaTyr: 0.423 ± 0.015	1.058XaaXaa: 1.058 ± 0.041

Statistics based on 4857 proteins (1731709 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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