Amino acid dipepetide frequency for Pseudogymnoascus sp. WSF 3629

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.03AlaAla: 10.03 ± 0.072	1.024AlaCys: 1.024 ± 0.014	4.395AlaAsp: 4.395 ± 0.033	5.494AlaGlu: 5.494 ± 0.047	3.15AlaPhe: 3.15 ± 0.029	6.211AlaGly: 6.211 ± 0.039	1.702AlaHis: 1.702 ± 0.017	4.489AlaIle: 4.489 ± 0.032	4.463AlaLys: 4.463 ± 0.041	7.912AlaLeu: 7.912 ± 0.052	1.998AlaMet: 1.998 ± 0.021	3.088AlaAsn: 3.088 ± 0.027	5.252AlaPro: 5.252 ± 0.044	3.417AlaGln: 3.417 ± 0.03	4.885AlaArg: 4.885 ± 0.034	7.231AlaSer: 7.231 ± 0.04	5.665AlaThr: 5.665 ± 0.039	5.618AlaVal: 5.618 ± 0.037	1.139AlaTrp: 1.139 ± 0.015	2.195AlaTyr: 2.195 ± 0.021	0.0AlaXaa: 0.0 ± 0.0
Cys	0.933CysAla: 0.933 ± 0.015	0.234CysCys: 0.234 ± 0.008	0.634CysAsp: 0.634 ± 0.014	0.566CysGlu: 0.566 ± 0.01	0.516CysPhe: 0.516 ± 0.011	0.96CysGly: 0.96 ± 0.018	0.301CysHis: 0.301 ± 0.008	0.683CysIle: 0.683 ± 0.011	0.5CysLys: 0.5 ± 0.011	1.149CysLeu: 1.149 ± 0.016	0.257CysMet: 0.257 ± 0.007	0.416CysAsn: 0.416 ± 0.009	0.603CysPro: 0.603 ± 0.015	0.399CysGln: 0.399 ± 0.009	0.666CysArg: 0.666 ± 0.012	0.833CysSer: 0.833 ± 0.015	0.681CysThr: 0.681 ± 0.011	0.744CysVal: 0.744 ± 0.013	0.192CysTrp: 0.192 ± 0.006	0.347CysTyr: 0.347 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	4.855AspAla: 4.855 ± 0.035	0.579AspCys: 0.579 ± 0.012	4.095AspAsp: 4.095 ± 0.04	4.508AspGlu: 4.508 ± 0.038	2.233AspPhe: 2.233 ± 0.021	4.241AspGly: 4.241 ± 0.03	1.106AspHis: 1.106 ± 0.015	3.195AspIle: 3.195 ± 0.024	2.439AspLys: 2.439 ± 0.024	4.915AspLeu: 4.915 ± 0.033	1.244AspMet: 1.244 ± 0.016	1.937AspAsn: 1.937 ± 0.021	3.051AspPro: 3.051 ± 0.028	1.703AspGln: 1.703 ± 0.019	2.823AspArg: 2.823 ± 0.027	4.061AspSer: 4.061 ± 0.033	2.978AspThr: 2.978 ± 0.026	3.681AspVal: 3.681 ± 0.031	0.847AspTrp: 0.847 ± 0.012	1.59AspTyr: 1.59 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	5.803GluAla: 5.803 ± 0.052	0.602GluCys: 0.602 ± 0.01	4.201GluAsp: 4.201 ± 0.039	5.956GluGlu: 5.956 ± 0.061	1.963GluPhe: 1.963 ± 0.021	4.44GluGly: 4.44 ± 0.036	1.244GluHis: 1.244 ± 0.015	3.162GluIle: 3.162 ± 0.026	3.865GluLys: 3.865 ± 0.035	5.213GluLeu: 5.213 ± 0.038	1.58GluMet: 1.58 ± 0.016	2.245GluAsn: 2.245 ± 0.024	2.672GluPro: 2.672 ± 0.035	2.301GluGln: 2.301 ± 0.027	4.035GluArg: 4.035 ± 0.037	4.276GluSer: 4.276 ± 0.033	3.438GluThr: 3.438 ± 0.029	3.953GluVal: 3.953 ± 0.032	0.896GluTrp: 0.896 ± 0.013	1.706GluTyr: 1.706 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	3.063PheAla: 3.063 ± 0.027	0.527PheCys: 0.527 ± 0.009	2.227PheAsp: 2.227 ± 0.021	2.194PheGlu: 2.194 ± 0.02	1.546PhePhe: 1.546 ± 0.02	2.942PheGly: 2.942 ± 0.031	0.826PheHis: 0.826 ± 0.014	1.834PheIle: 1.834 ± 0.02	1.576PheLys: 1.576 ± 0.018	3.287PheLeu: 3.287 ± 0.024	0.807PheMet: 0.807 ± 0.012	1.434PheAsn: 1.434 ± 0.016	1.875PhePro: 1.875 ± 0.021	1.336PheGln: 1.336 ± 0.018	1.805PheArg: 1.805 ± 0.02	2.921PheSer: 2.921 ± 0.024	2.141PheThr: 2.141 ± 0.019	2.346PheVal: 2.346 ± 0.023	0.625PheTrp: 0.625 ± 0.012	1.084PheTyr: 1.084 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	5.924GlyAla: 5.924 ± 0.044	0.884GlyCys: 0.884 ± 0.014	3.822GlyAsp: 3.822 ± 0.033	4.25GlyGlu: 4.25 ± 0.033	2.89GlyPhe: 2.89 ± 0.027	7.182GlyGly: 7.182 ± 0.063	1.684GlyHis: 1.684 ± 0.02	3.644GlyIle: 3.644 ± 0.028	3.886GlyLys: 3.886 ± 0.03	6.092GlyLeu: 6.092 ± 0.038	1.743GlyMet: 1.743 ± 0.021	2.762GlyAsn: 2.762 ± 0.024	3.353GlyPro: 3.353 ± 0.033	2.585GlyGln: 2.585 ± 0.027	4.284GlyArg: 4.284 ± 0.036	5.838GlySer: 5.838 ± 0.036	4.144GlyThr: 4.144 ± 0.033	4.742GlyVal: 4.742 ± 0.035	1.201GlyTrp: 1.201 ± 0.015	2.152GlyTyr: 2.152 ± 0.025	0.0GlyXaa: 0.0 ± 0.0
His	1.738HisAla: 1.738 ± 0.018	0.283HisCys: 0.283 ± 0.009	1.19HisAsp: 1.19 ± 0.015	1.217HisGlu: 1.217 ± 0.016	0.864HisPhe: 0.864 ± 0.013	1.623HisGly: 1.623 ± 0.02	0.71HisHis: 0.71 ± 0.014	1.217HisIle: 1.217 ± 0.016	0.908HisLys: 0.908 ± 0.012	2.098HisLeu: 2.098 ± 0.02	0.456HisMet: 0.456 ± 0.01	0.842HisAsn: 0.842 ± 0.012	1.534HisPro: 1.534 ± 0.021	0.875HisGln: 0.875 ± 0.015	1.348HisArg: 1.348 ± 0.017	1.668HisSer: 1.668 ± 0.019	1.239HisThr: 1.239 ± 0.015	1.336HisVal: 1.336 ± 0.016	0.303HisTrp: 0.303 ± 0.008	0.681HisTyr: 0.681 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	4.377IleAla: 4.377 ± 0.03	0.745IleCys: 0.745 ± 0.014	2.947IleAsp: 2.947 ± 0.025	3.004IleGlu: 3.004 ± 0.025	2.012IlePhe: 2.012 ± 0.019	3.31IleGly: 3.31 ± 0.031	1.149IleHis: 1.149 ± 0.015	2.694IleIle: 2.694 ± 0.027	2.321IleLys: 2.321 ± 0.024	4.585IleLeu: 4.585 ± 0.034	1.053IleMet: 1.053 ± 0.015	1.896IleAsn: 1.896 ± 0.019	3.066IlePro: 3.066 ± 0.024	1.869IleGln: 1.869 ± 0.019	2.69IleArg: 2.69 ± 0.021	4.01IleSer: 4.01 ± 0.029	3.065IleThr: 3.065 ± 0.023	3.236IleVal: 3.236 ± 0.028	0.705IleTrp: 0.705 ± 0.013	1.467IleTyr: 1.467 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	4.616LysAla: 4.616 ± 0.044	0.499LysCys: 0.499 ± 0.011	2.856LysAsp: 2.856 ± 0.027	3.609LysGlu: 3.609 ± 0.038	1.52LysPhe: 1.52 ± 0.02	3.359LysGly: 3.359 ± 0.028	1.087LysHis: 1.087 ± 0.015	2.41LysIle: 2.41 ± 0.026	3.471LysLys: 3.471 ± 0.043	4.273LysLeu: 4.273 ± 0.026	1.057LysMet: 1.057 ± 0.014	1.814LysAsn: 1.814 ± 0.019	2.722LysPro: 2.722 ± 0.027	1.761LysGln: 1.761 ± 0.017	3.344LysArg: 3.344 ± 0.029	3.672LysSer: 3.672 ± 0.03	2.943LysThr: 2.943 ± 0.029	3.013LysVal: 3.013 ± 0.027	0.693LysTrp: 0.693 ± 0.012	1.411LysTyr: 1.411 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	7.891LeuAla: 7.891 ± 0.049	1.115LeuCys: 1.115 ± 0.015	5.032LeuAsp: 5.032 ± 0.033	5.535LeuGlu: 5.535 ± 0.039	3.183LeuPhe: 3.183 ± 0.024	6.006LeuGly: 6.006 ± 0.031	2.101LeuHis: 2.101 ± 0.021	3.968LeuIle: 3.968 ± 0.029	4.395LeuLys: 4.395 ± 0.041	8.359LeuLeu: 8.359 ± 0.06	1.772LeuMet: 1.772 ± 0.021	3.092LeuAsn: 3.092 ± 0.026	5.418LeuPro: 5.418 ± 0.037	3.662LeuGln: 3.662 ± 0.031	5.391LeuArg: 5.391 ± 0.035	7.203LeuSer: 7.203 ± 0.044	4.856LeuThr: 4.856 ± 0.034	5.27LeuVal: 5.27 ± 0.034	1.16LeuTrp: 1.16 ± 0.016	2.285LeuTyr: 2.285 ± 0.021	0.0LeuXaa: 0.0 ± 0.0
Met	2.261MetAla: 2.261 ± 0.021	0.221MetCys: 0.221 ± 0.007	1.27MetAsp: 1.27 ± 0.015	1.386MetGlu: 1.386 ± 0.017	0.759MetPhe: 0.759 ± 0.012	1.647MetGly: 1.647 ± 0.019	0.478MetHis: 0.478 ± 0.01	0.98MetIle: 0.98 ± 0.014	1.068MetLys: 1.068 ± 0.012	1.812MetLeu: 1.812 ± 0.018	0.581MetMet: 0.581 ± 0.011	0.796MetAsn: 0.796 ± 0.013	1.229MetPro: 1.229 ± 0.017	0.819MetGln: 0.819 ± 0.015	1.249MetArg: 1.249 ± 0.013	1.878MetSer: 1.878 ± 0.019	1.23MetThr: 1.23 ± 0.015	1.348MetVal: 1.348 ± 0.015	0.269MetTrp: 0.269 ± 0.008	0.521MetTyr: 0.521 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.234AsnAla: 3.234 ± 0.026	0.441AsnCys: 0.441 ± 0.011	1.943AsnAsp: 1.943 ± 0.021	2.024AsnGlu: 2.024 ± 0.02	1.406AsnPhe: 1.406 ± 0.017	3.191AsnGly: 3.191 ± 0.031	0.794AsnHis: 0.794 ± 0.014	2.168AsnIle: 2.168 ± 0.023	1.598AsnLys: 1.598 ± 0.018	3.207AsnLeu: 3.207 ± 0.026	0.844AsnMet: 0.844 ± 0.014	1.578AsnAsn: 1.578 ± 0.024	2.461AsnPro: 2.461 ± 0.024	1.285AsnGln: 1.285 ± 0.019	1.872AsnArg: 1.872 ± 0.022	2.831AsnSer: 2.831 ± 0.031	2.279AsnThr: 2.279 ± 0.023	2.283AsnVal: 2.283 ± 0.023	0.547AsnTrp: 0.547 ± 0.01	1.109AsnTyr: 1.109 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	5.348ProAla: 5.348 ± 0.043	0.459ProCys: 0.459 ± 0.011	2.942ProAsp: 2.942 ± 0.028	3.607ProGlu: 3.607 ± 0.03	1.932ProPhe: 1.932 ± 0.02	3.915ProGly: 3.915 ± 0.037	1.25ProHis: 1.25 ± 0.015	2.624ProIle: 2.624 ± 0.022	2.768ProLys: 2.768 ± 0.027	4.783ProLeu: 4.783 ± 0.035	1.038ProMet: 1.038 ± 0.014	2.205ProAsn: 2.205 ± 0.025	5.166ProPro: 5.166 ± 0.07	2.409ProGln: 2.409 ± 0.034	3.29ProArg: 3.29 ± 0.033	5.801ProSer: 5.801 ± 0.045	4.278ProThr: 4.278 ± 0.038	3.515ProVal: 3.515 ± 0.03	0.706ProTrp: 0.706 ± 0.013	1.466ProTyr: 1.466 ± 0.019	0.0ProXaa: 0.0 ± 0.0
Gln	3.367GlnAla: 3.367 ± 0.027	0.408GlnCys: 0.408 ± 0.01	1.902GlnAsp: 1.902 ± 0.019	2.282GlnGlu: 2.282 ± 0.025	1.236GlnPhe: 1.236 ± 0.014	2.505GlnGly: 2.505 ± 0.027	0.945GlnHis: 0.945 ± 0.016	1.857GlnIle: 1.857 ± 0.018	1.922GlnLys: 1.922 ± 0.021	3.339GlnLeu: 3.339 ± 0.027	0.87GlnMet: 0.87 ± 0.014	1.52GlnAsn: 1.52 ± 0.018	2.35GlnPro: 2.35 ± 0.033	2.441GlnGln: 2.441 ± 0.059	2.439GlnArg: 2.439 ± 0.024	2.919GlnSer: 2.919 ± 0.026	2.21GlnThr: 2.21 ± 0.022	2.04GlnVal: 2.04 ± 0.022	0.544GlnTrp: 0.544 ± 0.011	1.157GlnTyr: 1.157 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	4.681ArgAla: 4.681 ± 0.04	0.66ArgCys: 0.66 ± 0.014	3.249ArgAsp: 3.249 ± 0.029	3.935ArgGlu: 3.935 ± 0.039	1.973ArgPhe: 1.973 ± 0.02	3.974ArgGly: 3.974 ± 0.04	1.409ArgHis: 1.409 ± 0.015	2.813ArgIle: 2.813 ± 0.024	3.325ArgLys: 3.325 ± 0.026	5.078ArgLeu: 5.078 ± 0.034	1.289ArgMet: 1.289 ± 0.015	2.176ArgAsn: 2.176 ± 0.022	3.214ArgPro: 3.214 ± 0.032	2.433ArgGln: 2.433 ± 0.027	4.781ArgArg: 4.781 ± 0.048	4.372ArgSer: 4.372 ± 0.036	3.154ArgThr: 3.154 ± 0.025	3.295ArgVal: 3.295 ± 0.027	0.839ArgTrp: 0.839 ± 0.015	1.542ArgTyr: 1.542 ± 0.016	0.0ArgXaa: 0.0 ± 0.0
Ser	6.705SerAla: 6.705 ± 0.037	0.838SerCys: 0.838 ± 0.015	4.148SerAsp: 4.148 ± 0.029	4.148SerGlu: 4.148 ± 0.035	2.927SerPhe: 2.927 ± 0.025	5.719SerGly: 5.719 ± 0.041	1.796SerHis: 1.796 ± 0.021	4.078SerIle: 4.078 ± 0.03	3.831SerLys: 3.831 ± 0.031	7.079SerLeu: 7.079 ± 0.038	1.694SerMet: 1.694 ± 0.018	3.058SerAsn: 3.058 ± 0.029	5.497SerPro: 5.497 ± 0.046	3.09SerGln: 3.09 ± 0.028	4.626SerArg: 4.626 ± 0.036	8.234SerSer: 8.234 ± 0.065	5.585SerThr: 5.585 ± 0.041	4.504SerVal: 4.504 ± 0.033	1.077SerTrp: 1.077 ± 0.014	2.086SerTyr: 2.086 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	5.453ThrAla: 5.453 ± 0.035	0.743ThrCys: 0.743 ± 0.012	2.9ThrAsp: 2.9 ± 0.024	3.185ThrGlu: 3.185 ± 0.028	2.245ThrPhe: 2.245 ± 0.021	4.331ThrGly: 4.331 ± 0.032	1.265ThrHis: 1.265 ± 0.016	3.242ThrIle: 3.242 ± 0.024	2.778ThrLys: 2.778 ± 0.027	5.249ThrLeu: 5.249 ± 0.036	1.173ThrMet: 1.173 ± 0.015	2.185ThrAsn: 2.185 ± 0.022	4.558ThrPro: 4.558 ± 0.038	2.038ThrGln: 2.038 ± 0.019	2.981ThrArg: 2.981 ± 0.027	5.417ThrSer: 5.417 ± 0.036	4.738ThrThr: 4.738 ± 0.048	3.747ThrVal: 3.747 ± 0.033	0.846ThrTrp: 0.846 ± 0.013	1.677ThrTyr: 1.677 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	5.695ValAla: 5.695 ± 0.041	0.764ValCys: 0.764 ± 0.013	3.771ValAsp: 3.771 ± 0.029	4.238ValGlu: 4.238 ± 0.034	2.361ValPhe: 2.361 ± 0.023	4.368ValGly: 4.368 ± 0.036	1.273ValHis: 1.273 ± 0.016	2.989ValIle: 2.989 ± 0.029	3.093ValLys: 3.093 ± 0.028	5.44ValLeu: 5.44 ± 0.036	1.365ValMet: 1.365 ± 0.015	2.244ValAsn: 2.244 ± 0.019	3.463ValPro: 3.463 ± 0.029	2.254ValGln: 2.254 ± 0.021	3.303ValArg: 3.303 ± 0.028	4.488ValSer: 4.488 ± 0.03	3.556ValThr: 3.556 ± 0.028	4.674ValVal: 4.674 ± 0.041	0.853ValTrp: 0.853 ± 0.012	1.679ValTyr: 1.679 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	1.17TrpAla: 1.17 ± 0.017	0.179TrpCys: 0.179 ± 0.006	0.887TrpAsp: 0.887 ± 0.013	0.866TrpGlu: 0.866 ± 0.014	0.518TrpPhe: 0.518 ± 0.011	1.0TrpGly: 1.0 ± 0.017	0.322TrpHis: 0.322 ± 0.008	0.723TrpIle: 0.723 ± 0.013	0.768TrpLys: 0.768 ± 0.013	1.251TrpLeu: 1.251 ± 0.018	0.369TrpMet: 0.369 ± 0.009	0.608TrpAsn: 0.608 ± 0.011	0.572TrpPro: 0.572 ± 0.01	0.503TrpGln: 0.503 ± 0.009	0.887TrpArg: 0.887 ± 0.012	1.006TrpSer: 1.006 ± 0.016	0.893TrpThr: 0.893 ± 0.013	0.92TrpVal: 0.92 ± 0.013	0.265TrpTrp: 0.265 ± 0.008	0.412TrpTyr: 0.412 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.215TyrAla: 2.215 ± 0.024	0.408TyrCys: 0.408 ± 0.009	1.67TyrAsp: 1.67 ± 0.018	1.549TyrGlu: 1.549 ± 0.018	1.162TyrPhe: 1.162 ± 0.014	2.135TyrGly: 2.135 ± 0.021	0.685TyrHis: 0.685 ± 0.011	1.429TyrIle: 1.429 ± 0.017	1.157TyrLys: 1.157 ± 0.015	2.573TyrLeu: 2.573 ± 0.024	0.613TyrMet: 0.613 ± 0.011	1.198TyrAsn: 1.198 ± 0.017	1.445TyrPro: 1.445 ± 0.021	1.078TyrGln: 1.078 ± 0.015	1.493TyrArg: 1.493 ± 0.017	2.062TyrSer: 2.062 ± 0.021	1.678TyrThr: 1.678 ± 0.019	1.603TyrVal: 1.603 ± 0.022	0.414TyrTrp: 0.414 ± 0.009	0.916TyrTyr: 0.916 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 11000 proteins (5350055 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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