Sparrow coronavirus HKU17

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Deltacoronavirus; Buldecovirus; unclassified Buldecovirus

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9BR04|H9BR04_9NIDO Nucleocapsid protein OS=Sparrow coronavirus HKU17 OX=1159906 GN=N PE=4 SV=1
MM1 pKa = 7.59CNCLLQLRR9 pKa = 11.84EE10 pKa = 4.25LYY12 pKa = 10.43KK13 pKa = 10.9LCNEE17 pKa = 4.26RR18 pKa = 11.84NITRR22 pKa = 11.84DD23 pKa = 3.51DD24 pKa = 3.7VLEE27 pKa = 5.34LIDD30 pKa = 4.45PLIKK34 pKa = 9.74TRR36 pKa = 11.84CFAYY40 pKa = 10.63SLVVLANANPIALSILPRR58 pKa = 11.84KK59 pKa = 9.53ILINGEE65 pKa = 4.19PLLLEE70 pKa = 4.08YY71 pKa = 11.25GNIYY75 pKa = 10.59GKK77 pKa = 10.45DD78 pKa = 3.43FLYY81 pKa = 10.53RR82 pKa = 11.84PSLQVILEE90 pKa = 4.12EE91 pKa = 4.63EE92 pKa = 4.27EE93 pKa = 4.51LNN95 pKa = 4.07

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9BR05|H9BR05_9NIDO NS7a protein OS=Sparrow coronavirus HKU17 OX=1159906 GN=NS7a PE=4 SV=1
MM1 pKa = 7.44ATPSVPTTDD10 pKa = 4.06ASWFQVLKK18 pKa = 10.58AQNKK22 pKa = 9.03KK23 pKa = 8.6ATHH26 pKa = 5.19PQFRR30 pKa = 11.84GNGVPLNSAIKK41 pKa = 8.82PAEE44 pKa = 3.75NHH46 pKa = 6.51GYY48 pKa = 7.44WLRR51 pKa = 11.84YY52 pKa = 7.01TRR54 pKa = 11.84QKK56 pKa = 10.76PGGTQIPPSYY66 pKa = 10.58AFYY69 pKa = 9.95YY70 pKa = 9.86TGTGPRR76 pKa = 11.84GNLKK80 pKa = 10.6YY81 pKa = 11.01GEE83 pKa = 4.87LPPNDD88 pKa = 3.7TQATTRR94 pKa = 11.84VTWVKK99 pKa = 10.92GPGADD104 pKa = 3.37TSIKK108 pKa = 9.68PHH110 pKa = 4.45VAKK113 pKa = 10.68RR114 pKa = 11.84NPNNPKK120 pKa = 9.85HH121 pKa = 5.96QLLPLRR127 pKa = 11.84FPTGDD132 pKa = 3.2GPAQGFRR139 pKa = 11.84VDD141 pKa = 3.64PFNARR146 pKa = 11.84GRR148 pKa = 11.84PQEE151 pKa = 4.34RR152 pKa = 11.84GSGPRR157 pKa = 11.84SQSANPRR164 pKa = 11.84GTNNQPRR171 pKa = 11.84KK172 pKa = 9.28RR173 pKa = 11.84DD174 pKa = 3.4QSAPAAVRR182 pKa = 11.84RR183 pKa = 11.84KK184 pKa = 7.09TQHH187 pKa = 5.18QAPKK191 pKa = 9.09RR192 pKa = 11.84TLPKK196 pKa = 10.35GKK198 pKa = 8.69TISQVFGNRR207 pKa = 11.84SRR209 pKa = 11.84TGANVGSADD218 pKa = 3.79TEE220 pKa = 4.29KK221 pKa = 10.47TGMADD226 pKa = 3.45PRR228 pKa = 11.84IMALARR234 pKa = 11.84HH235 pKa = 5.28VPGVQEE241 pKa = 3.98MLFAGHH247 pKa = 7.25LEE249 pKa = 4.47SNFQAGAITLTFSYY263 pKa = 10.44SITVKK268 pKa = 10.43EE269 pKa = 4.05GSPDD273 pKa = 3.52YY274 pKa = 11.08EE275 pKa = 3.99RR276 pKa = 11.84LKK278 pKa = 11.02DD279 pKa = 3.7ALNTVVNQTYY289 pKa = 9.4EE290 pKa = 4.24PPTKK294 pKa = 8.96PTKK297 pKa = 10.18DD298 pKa = 3.39KK299 pKa = 11.2KK300 pKa = 10.44PEE302 pKa = 4.08KK303 pKa = 9.57QDD305 pKa = 3.24QSAKK309 pKa = 10.18PKK311 pKa = 8.42QQKK314 pKa = 9.59KK315 pKa = 8.18PKK317 pKa = 9.94KK318 pKa = 8.31VTLPADD324 pKa = 3.79KK325 pKa = 10.5QDD327 pKa = 4.98LEE329 pKa = 4.12WDD331 pKa = 3.51DD332 pKa = 4.14AFEE335 pKa = 4.59IKK337 pKa = 10.2QEE339 pKa = 4.19SAAA342 pKa = 4.0

Molecular weight: 37.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

8433

70

6277

1054.1

117.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.909 ± 0.449

2.561 ± 0.335

5.277 ± 0.592

4.162 ± 0.318

4.316 ± 0.317

5.182 ± 0.541

2.502 ± 0.29

5.977 ± 0.663

5.052 ± 0.622

9.321 ± 0.578

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.719 ± 0.248

5.443 ± 0.535

5.099 ± 0.9

4.328 ± 0.391

3.581 ± 0.463

6.143 ± 0.628

7.862 ± 0.795

7.791 ± 0.881

0.901 ± 0.384

4.838 ± 0.367
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski