Hubei tombus-like virus 14

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGE5|A0A1L3KGE5_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 14 OX=1923260 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.21TPDD5 pKa = 3.67PQVNSAEE12 pKa = 3.98WKK14 pKa = 9.38QFRR17 pKa = 11.84KK18 pKa = 9.9VLRR21 pKa = 11.84EE22 pKa = 3.77LSDD25 pKa = 3.59EE26 pKa = 4.28VGSVPKK32 pKa = 9.55ATPTTVVNHH41 pKa = 6.13RR42 pKa = 11.84NSMKK46 pKa = 10.06KK47 pKa = 9.61RR48 pKa = 11.84RR49 pKa = 11.84FGKK52 pKa = 10.44GMEE55 pKa = 3.76IYY57 pKa = 10.52LRR59 pKa = 11.84EE60 pKa = 4.01GVAAKK65 pKa = 10.22HH66 pKa = 6.27SYY68 pKa = 7.71ITMMPKK74 pKa = 10.38LEE76 pKa = 4.87FYY78 pKa = 10.72DD79 pKa = 3.99SEE81 pKa = 4.62KK82 pKa = 10.74IPLKK86 pKa = 9.87EE87 pKa = 4.25DD88 pKa = 2.72RR89 pKa = 11.84GIQYY93 pKa = 10.04RR94 pKa = 11.84SPVYY98 pKa = 9.31NAALARR104 pKa = 11.84HH105 pKa = 5.67LHH107 pKa = 5.59HH108 pKa = 8.33VEE110 pKa = 3.6EE111 pKa = 4.28QLYY114 pKa = 8.12KK115 pKa = 10.18TIKK118 pKa = 10.47NVDD121 pKa = 3.59GTPVFAKK128 pKa = 10.05GYY130 pKa = 9.75SPMEE134 pKa = 3.78RR135 pKa = 11.84ALIIDD140 pKa = 4.04AMASRR145 pKa = 11.84FKK147 pKa = 11.26EE148 pKa = 4.13PMFLLADD155 pKa = 3.41HH156 pKa = 6.87HH157 pKa = 7.03RR158 pKa = 11.84FDD160 pKa = 3.65AHH162 pKa = 7.03VNGPLLDD169 pKa = 3.97EE170 pKa = 3.98EE171 pKa = 4.42HH172 pKa = 7.09RR173 pKa = 11.84FYY175 pKa = 11.27LRR177 pKa = 11.84CRR179 pKa = 11.84RR180 pKa = 11.84WNLEE184 pKa = 3.61LRR186 pKa = 11.84QLLEE190 pKa = 3.86WQKK193 pKa = 10.56KK194 pKa = 8.06NKK196 pKa = 9.39GVSHH200 pKa = 6.53GGGRR204 pKa = 11.84YY205 pKa = 8.97RR206 pKa = 11.84MRR208 pKa = 11.84AKK210 pKa = 10.56RR211 pKa = 11.84MSGDD215 pKa = 3.71LNTSLGNSVINYY227 pKa = 8.31GVLKK231 pKa = 10.53AFCKK235 pKa = 10.34HH236 pKa = 5.46FNIDD240 pKa = 2.58ASMFIDD246 pKa = 4.43GDD248 pKa = 3.85DD249 pKa = 4.46SIMIMEE255 pKa = 4.69KK256 pKa = 9.82QHH258 pKa = 7.18LPDD261 pKa = 3.9LVAFAEE267 pKa = 4.27KK268 pKa = 10.19FGLVTEE274 pKa = 4.47VEE276 pKa = 4.58VVHH279 pKa = 7.08DD280 pKa = 3.48IRR282 pKa = 11.84HH283 pKa = 6.14AEE285 pKa = 4.13FCQSRR290 pKa = 11.84IIYY293 pKa = 9.51LEE295 pKa = 4.08KK296 pKa = 10.58GPIMVRR302 pKa = 11.84NPWKK306 pKa = 10.78IMDD309 pKa = 4.67CMCKK313 pKa = 9.9SPRR316 pKa = 11.84KK317 pKa = 9.66LRR319 pKa = 11.84PEE321 pKa = 3.73QARR324 pKa = 11.84GVLAATALCEE334 pKa = 4.31LMQSPGVPVIAPCASALLTYY354 pKa = 10.82AGGKK358 pKa = 9.54PMFITPTAWGKK369 pKa = 8.85FQGWQTDD376 pKa = 3.73QIVDD380 pKa = 3.52IVDD383 pKa = 3.53EE384 pKa = 4.27SARR387 pKa = 11.84ADD389 pKa = 4.46LEE391 pKa = 4.49FAWGMTISEE400 pKa = 4.12QLMMEE405 pKa = 4.51EE406 pKa = 4.88HH407 pKa = 6.19YY408 pKa = 10.91KK409 pKa = 10.4HH410 pKa = 6.02YY411 pKa = 10.73ARR413 pKa = 11.84EE414 pKa = 4.12GVTIQMPNPKK424 pKa = 9.65SKK426 pKa = 10.36PKK428 pKa = 10.22EE429 pKa = 3.7IEE431 pKa = 3.74FEE433 pKa = 3.64IWDD436 pKa = 4.22AYY438 pKa = 7.39QTQYY442 pKa = 11.25QPTEE446 pKa = 3.79VRR448 pKa = 11.84RR449 pKa = 11.84WWRR452 pKa = 11.84DD453 pKa = 2.53RR454 pKa = 11.84WEE456 pKa = 3.96ISQYY460 pKa = 10.94LPPLDD465 pKa = 4.48PEE467 pKa = 4.01AVYY470 pKa = 10.66YY471 pKa = 11.08AEE473 pKa = 4.98LLGG476 pKa = 4.35

Molecular weight: 55.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG86|A0A1L3KG86_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 14 OX=1923260 PE=4 SV=1
MM1 pKa = 6.94FQLFEE6 pKa = 5.3SEE8 pKa = 4.31LQTCRR13 pKa = 11.84GCGNIARR20 pKa = 11.84VYY22 pKa = 10.44CEE24 pKa = 4.15KK25 pKa = 10.58CRR27 pKa = 11.84QSYY30 pKa = 10.76AKK32 pKa = 10.38YY33 pKa = 10.53DD34 pKa = 3.93LNTKK38 pKa = 9.22EE39 pKa = 3.86RR40 pKa = 11.84LGRR43 pKa = 11.84RR44 pKa = 11.84SYY46 pKa = 8.8WCRR49 pKa = 11.84AKK51 pKa = 10.4RR52 pKa = 11.84SHH54 pKa = 6.97PLAEE58 pKa = 3.87EE59 pKa = 4.44AIYY62 pKa = 10.45RR63 pKa = 11.84PEE65 pKa = 4.35AQIPWCEE72 pKa = 4.36CKK74 pKa = 10.54RR75 pKa = 11.84LDD77 pKa = 4.07GSPMPGLQEE86 pKa = 4.17KK87 pKa = 9.36QQQQQQQAQQQPQQQTYY104 pKa = 10.92APIQPISEE112 pKa = 4.2QKK114 pKa = 10.32LAAAKK119 pKa = 9.85AQAAASTTTSQQQQIRR135 pKa = 11.84DD136 pKa = 3.67EE137 pKa = 4.61HH138 pKa = 6.0GTWPTKK144 pKa = 9.91SAARR148 pKa = 11.84QAHH151 pKa = 4.64YY152 pKa = 9.5QARR155 pKa = 11.84AVGWRR160 pKa = 11.84EE161 pKa = 3.64LQRR164 pKa = 11.84EE165 pKa = 4.21KK166 pKa = 11.26NKK168 pKa = 10.12ARR170 pKa = 11.84RR171 pKa = 11.84QEE173 pKa = 4.17SKK175 pKa = 10.82VLSGTQSMGKK185 pKa = 8.98QRR187 pKa = 11.84PFRR190 pKa = 11.84APPAVSPVRR199 pKa = 11.84GQRR202 pKa = 11.84FEE204 pKa = 4.08QVQCASCGGSGSMWLYY220 pKa = 10.25QPIYY224 pKa = 10.75RR225 pKa = 11.84NDD227 pKa = 3.37PPYY230 pKa = 11.3NRR232 pKa = 11.84GGAKK236 pKa = 10.32AKK238 pKa = 9.91GQRR241 pKa = 11.84LDD243 pKa = 3.25QTAAASTTNQQQQHH257 pKa = 5.02QQQQQQPSSPYY268 pKa = 7.9TKK270 pKa = 10.31AFLRR274 pKa = 11.84RR275 pKa = 11.84EE276 pKa = 3.46RR277 pKa = 11.84RR278 pKa = 11.84RR279 pKa = 11.84RR280 pKa = 11.84QKK282 pKa = 10.4QYY284 pKa = 9.91YY285 pKa = 8.85EE286 pKa = 3.85EE287 pKa = 4.25EE288 pKa = 3.65ARR290 pKa = 11.84QRR292 pKa = 11.84LEE294 pKa = 4.4GMGQRR299 pKa = 11.84LGNPEE304 pKa = 3.88QNDD307 pKa = 3.48EE308 pKa = 4.28RR309 pKa = 11.84DD310 pKa = 3.54QPPAGQSEE318 pKa = 4.43EE319 pKa = 3.77QGGIRR324 pKa = 11.84EE325 pKa = 4.35CRR327 pKa = 11.84VEE329 pKa = 4.49EE330 pKa = 4.25ATCPDD335 pKa = 3.85AGASDD340 pKa = 3.76IAPRR344 pKa = 11.84CPSKK348 pKa = 11.13SPARR352 pKa = 11.84CGGSPGAKK360 pKa = 9.22PSTDD364 pKa = 2.86RR365 pKa = 11.84TSEE368 pKa = 4.16SNPRR372 pKa = 11.84SASPEE377 pKa = 3.78ARR379 pKa = 11.84PAPCSALAGEE389 pKa = 4.65HH390 pKa = 6.54GEE392 pKa = 4.07PLALPGASEE401 pKa = 4.17QPKK404 pKa = 10.14SSRR407 pKa = 11.84GNQRR411 pKa = 11.84TRR413 pKa = 11.84RR414 pKa = 11.84NRR416 pKa = 11.84RR417 pKa = 11.84KK418 pKa = 9.35IAATLPHH425 pKa = 6.32QPHH428 pKa = 6.54SPTKK432 pKa = 9.62GSAGKK437 pKa = 10.12DD438 pKa = 3.06VLAPSDD444 pKa = 3.8EE445 pKa = 4.66GCGDD449 pKa = 3.41RR450 pKa = 11.84YY451 pKa = 11.15DD452 pKa = 3.53RR453 pKa = 11.84AIRR456 pKa = 11.84QLGSVEE462 pKa = 4.13QPGSEE467 pKa = 4.22SEE469 pKa = 4.29GGFDD473 pKa = 4.91EE474 pKa = 5.33PEE476 pKa = 3.81VDD478 pKa = 5.4AKK480 pKa = 11.34SCDD483 pKa = 3.39GRR485 pKa = 11.84DD486 pKa = 3.14

Molecular weight: 54.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1649

476

687

549.7

62.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.126 ± 0.831

2.183 ± 0.387

4.366 ± 0.27

7.641 ± 0.112

2.365 ± 0.53

6.125 ± 0.685

2.608 ± 0.407

3.457 ± 0.724

6.367 ± 0.495

6.186 ± 0.698

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.79 ± 0.726

4.73 ± 1.534

6.853 ± 0.488

8.187 ± 1.685

8.126 ± 0.813

6.61 ± 0.691

4.669 ± 0.597

4.548 ± 0.864

1.455 ± 0.309

2.608 ± 0.65
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski