Honeysuckle yellow vein mosaic virus-[Japan:Miyazaki:2001]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Tomato leaf curl Japan virus

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4F1V7|A4F1V7_9GEMI C4 OS=Honeysuckle yellow vein mosaic virus-[Japan:Miyazaki:2001] OX=435487 GN=C4 PE=3 SV=1
MM1 pKa = 7.3PTPNRR6 pKa = 11.84FKK8 pKa = 10.9INAKK12 pKa = 10.28NYY14 pKa = 8.33FLTYY18 pKa = 7.85PHH20 pKa = 7.15CSLTKK25 pKa = 10.57EE26 pKa = 4.09EE27 pKa = 4.94ALSQIQALQTPTNKK41 pKa = 10.07LFIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.54LHH51 pKa = 6.34EE52 pKa = 5.86DD53 pKa = 4.22GSPHH57 pKa = 6.11LHH59 pKa = 6.16VLIQFEE65 pKa = 4.48GKK67 pKa = 7.96YY68 pKa = 7.57TCRR71 pKa = 11.84NQRR74 pKa = 11.84FFDD77 pKa = 3.9LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.34SSSDD99 pKa = 2.92VKK101 pKa = 10.47TYY103 pKa = 8.75MEE105 pKa = 4.98KK106 pKa = 10.87DD107 pKa = 3.11GDD109 pKa = 3.86ILDD112 pKa = 4.21FGVFQVDD119 pKa = 3.16GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.26AYY134 pKa = 10.9AEE136 pKa = 4.72AINSGSKK143 pKa = 10.37SSALSILRR151 pKa = 11.84EE152 pKa = 4.12KK153 pKa = 10.79APKK156 pKa = 10.11DD157 pKa = 3.66YY158 pKa = 10.78ILQFHH163 pKa = 6.68NLNSNLDD170 pKa = 3.99RR171 pKa = 11.84IFAPLLEE178 pKa = 4.65EE179 pKa = 4.2FVSPFLSSSFDD190 pKa = 3.4QVPEE194 pKa = 3.89QLEE197 pKa = 3.83EE198 pKa = 3.73WAAEE202 pKa = 4.04NVRR205 pKa = 11.84DD206 pKa = 3.83SAARR210 pKa = 11.84PWRR213 pKa = 11.84PMSIVIEE220 pKa = 4.37GDD222 pKa = 3.13SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLHH235 pKa = 5.71PRR237 pKa = 11.84HH238 pKa = 6.41NYY240 pKa = 10.01LCGHH244 pKa = 7.41LDD246 pKa = 4.18LSPKK250 pKa = 10.02VYY252 pKa = 10.89SNEE255 pKa = 3.02AWYY258 pKa = 10.82NVIDD262 pKa = 5.29DD263 pKa = 4.41VDD265 pKa = 3.61PHH267 pKa = 5.85YY268 pKa = 11.0LKK270 pKa = 10.7HH271 pKa = 6.2FKK273 pKa = 10.7EE274 pKa = 4.46FMGAQRR280 pKa = 11.84DD281 pKa = 3.81WQSNTKK287 pKa = 9.26YY288 pKa = 10.63GKK290 pKa = 9.49PIQIKK295 pKa = 10.42GGIPTIFLCNPGPTSSYY312 pKa = 10.2TEE314 pKa = 3.95YY315 pKa = 11.29LNEE318 pKa = 4.3EE319 pKa = 4.15KK320 pKa = 10.63NASLKK325 pKa = 10.36HH326 pKa = 4.72WAIKK330 pKa = 10.32NATFVTLYY338 pKa = 10.64GPLYY342 pKa = 10.43SGTHH346 pKa = 5.08QSPAQDD352 pKa = 3.49RR353 pKa = 11.84EE354 pKa = 4.38EE355 pKa = 4.01EE356 pKa = 4.26TTPQEE361 pKa = 3.77EE362 pKa = 4.48DD363 pKa = 2.94

Molecular weight: 41.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4F1V4|A4F1V4_9GEMI Replication enhancer OS=Honeysuckle yellow vein mosaic virus-[Japan:Miyazaki:2001] OX=435487 GN=C3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.31RR4 pKa = 11.84PADD7 pKa = 3.24IGIFTPVSRR16 pKa = 11.84ARR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.19SPLLSRR30 pKa = 11.84ASAPIALVTNKK41 pKa = 9.36KK42 pKa = 10.22RR43 pKa = 11.84SWAQRR48 pKa = 11.84PMYY51 pKa = 9.61RR52 pKa = 11.84KK53 pKa = 8.23PKK55 pKa = 7.7MYY57 pKa = 10.61RR58 pKa = 11.84MYY60 pKa = 10.52RR61 pKa = 11.84SPDD64 pKa = 3.03IPKK67 pKa = 10.44GCEE70 pKa = 4.25GPCKK74 pKa = 10.24VQSFEE79 pKa = 4.27KK80 pKa = 10.39KK81 pKa = 10.29DD82 pKa = 3.76DD83 pKa = 3.74VGHH86 pKa = 5.99SGKK89 pKa = 8.94MLCISDD95 pKa = 3.41ITRR98 pKa = 11.84GNGLNHH104 pKa = 6.76RR105 pKa = 11.84VGKK108 pKa = 10.36RR109 pKa = 11.84FGIKK113 pKa = 9.42SVYY116 pKa = 10.21IMGKK120 pKa = 8.25IWMDD124 pKa = 3.37EE125 pKa = 3.97NIKK128 pKa = 10.65LKK130 pKa = 10.75NHH132 pKa = 5.62TNNVLFWLVRR142 pKa = 11.84DD143 pKa = 4.05RR144 pKa = 11.84RR145 pKa = 11.84PVTTPYY151 pKa = 11.16AFQEE155 pKa = 4.18AFNMFEE161 pKa = 4.62MEE163 pKa = 4.57PSTATIKK170 pKa = 10.46QDD172 pKa = 3.41LRR174 pKa = 11.84DD175 pKa = 3.81RR176 pKa = 11.84LQVLHH181 pKa = 6.81KK182 pKa = 10.2FSATVTGGQYY192 pKa = 10.98ASRR195 pKa = 11.84EE196 pKa = 3.67QALIKK201 pKa = 10.5RR202 pKa = 11.84FWKK205 pKa = 10.38LNHH208 pKa = 6.28HH209 pKa = 5.5VTYY212 pKa = 10.79NHH214 pKa = 6.09QEE216 pKa = 3.49AAKK219 pKa = 10.4YY220 pKa = 9.25EE221 pKa = 4.13NHH223 pKa = 6.46TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.77ATLKK246 pKa = 9.27IRR248 pKa = 11.84VYY250 pKa = 10.62FYY252 pKa = 11.1DD253 pKa = 3.7SEE255 pKa = 4.53QNN257 pKa = 3.27

Molecular weight: 29.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1145

116

363

190.8

21.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.764 ± 0.595

1.834 ± 0.367

4.454 ± 0.551

5.328 ± 0.632

4.541 ± 0.505

4.454 ± 0.417

4.454 ± 0.505

5.59 ± 0.543

5.852 ± 0.558

7.948 ± 0.457

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.414

5.153 ± 0.6

5.852 ± 0.533

5.328 ± 0.581

6.114 ± 0.621

8.646 ± 1.217

6.026 ± 0.546

4.891 ± 0.777

1.31 ± 0.19

4.105 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski