Streptococcus phage phi3396

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3F666|A3F666_9CAUD Uncharacterized protein OS=Streptococcus phage phi3396 OX=423476 GN=phi3396_57 PE=4 SV=1
MM1 pKa = 7.2ITGKK5 pKa = 9.93VNNMATLDD13 pKa = 3.86EE14 pKa = 4.43VLSFAKK20 pKa = 10.53GLADD24 pKa = 3.48TGQGVDD30 pKa = 3.78LDD32 pKa = 4.08NVYY35 pKa = 8.53GTQCVDD41 pKa = 3.5LPNWITTKK49 pKa = 10.75YY50 pKa = 10.5FGIALWGNAIDD61 pKa = 5.11LLDD64 pKa = 3.86SAAAQGMEE72 pKa = 4.21VVYY75 pKa = 10.3NAPGVNPRR83 pKa = 11.84AGAIFVMVTYY93 pKa = 10.75AHH95 pKa = 7.21GYY97 pKa = 7.86GHH99 pKa = 7.1TGLVIVTSDD108 pKa = 3.56GYY110 pKa = 10.61VLHH113 pKa = 7.03NIEE116 pKa = 4.75QNVDD120 pKa = 3.04GNADD124 pKa = 3.33ALYY127 pKa = 9.95IGGPARR133 pKa = 11.84YY134 pKa = 9.17VDD136 pKa = 4.39RR137 pKa = 11.84PFEE140 pKa = 5.2DD141 pKa = 3.37GTGYY145 pKa = 10.44ILGWFYY151 pKa = 10.78PPYY154 pKa = 10.83DD155 pKa = 3.68NTPVQEE161 pKa = 4.55TEE163 pKa = 3.58PSAPVVAQSDD173 pKa = 3.67GTYY176 pKa = 10.39VVNPEE181 pKa = 3.95TGTFTVRR188 pKa = 11.84VAALNVRR195 pKa = 11.84SAPRR199 pKa = 11.84LDD201 pKa = 3.6AEE203 pKa = 4.28IVATYY208 pKa = 9.87GEE210 pKa = 4.27NMEE213 pKa = 4.57FNYY216 pKa = 10.77DD217 pKa = 3.03GWIDD221 pKa = 3.51SDD223 pKa = 4.15GYY225 pKa = 10.69IWVTYY230 pKa = 10.35ISVTGVRR237 pKa = 11.84RR238 pKa = 11.84YY239 pKa = 9.74VAVGNSQNGRR249 pKa = 11.84RR250 pKa = 11.84VTNFGTFRR258 pKa = 4.37

Molecular weight: 28.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3F667|A3F667_9CAUD Uncharacterized protein OS=Streptococcus phage phi3396 OX=423476 GN=phi3396_58 PE=4 SV=1
MM1 pKa = 7.72FIYY4 pKa = 10.35IIRR7 pKa = 11.84RR8 pKa = 11.84NKK10 pKa = 8.92MEE12 pKa = 4.21YY13 pKa = 9.84GNKK16 pKa = 9.44IFEE19 pKa = 4.64IYY21 pKa = 10.45NKK23 pKa = 9.36PFKK26 pKa = 10.45YY27 pKa = 10.16RR28 pKa = 11.84NSSSTNYY35 pKa = 10.56NKK37 pKa = 9.42VRR39 pKa = 11.84ANGIEE44 pKa = 4.18PNTKK48 pKa = 9.66FVVNKK53 pKa = 10.24ASNINCAVYY62 pKa = 9.41PRR64 pKa = 11.84HH65 pKa = 6.17GSIEE69 pKa = 4.07KK70 pKa = 9.81VFYY73 pKa = 10.15WGDD76 pKa = 3.35RR77 pKa = 11.84KK78 pKa = 8.82ITQATAEE85 pKa = 4.24KK86 pKa = 9.87RR87 pKa = 11.84CGYY90 pKa = 10.49FKK92 pKa = 11.29GG93 pKa = 3.73

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12043

38

1375

188.2

21.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.643 ± 0.721

0.648 ± 0.119

6.51 ± 0.282

7.772 ± 0.35

3.961 ± 0.241

5.829 ± 0.374

1.312 ± 0.164

6.892 ± 0.219

9.715 ± 0.398

8.76 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.194

5.63 ± 0.254

2.4 ± 0.17

4.102 ± 0.253

4.434 ± 0.242

6.053 ± 0.429

5.746 ± 0.244

5.854 ± 0.293

1.196 ± 0.105

3.903 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski