Brassica napus (Rape)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60180 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A078JP97|A0A078JP97_BRANA BnaAnng23820D protein OS=Brassica napus OX=3708 GN=BnaAnng23820D PE=4 SV=1
MM1 pKa = 8.0VSITHH6 pKa = 6.91KK7 pKa = 10.58DD8 pKa = 3.51DD9 pKa = 5.11EE10 pKa = 4.55IVEE13 pKa = 4.57SKK15 pKa = 10.62EE16 pKa = 3.6FTPISSPKK24 pKa = 8.63NTYY27 pKa = 7.88DD28 pKa = 3.6TNSDD32 pKa = 3.79EE33 pKa = 6.11LNDD36 pKa = 3.67TDD38 pKa = 4.29EE39 pKa = 4.9EE40 pKa = 4.36EE41 pKa = 4.25VHH43 pKa = 7.24KK44 pKa = 10.58DD45 pKa = 2.99FCLYY49 pKa = 7.69YY50 pKa = 10.57CCMAVEE56 pKa = 4.96LEE58 pKa = 4.36DD59 pKa = 4.61APEE62 pKa = 3.75RR63 pKa = 11.84WSCNDD68 pKa = 3.57CVEE71 pKa = 4.89DD72 pKa = 4.07DD73 pKa = 4.37QIVEE77 pKa = 4.43SKK79 pKa = 10.3EE80 pKa = 3.45ITPISSPKK88 pKa = 9.15NTDD91 pKa = 3.18DD92 pKa = 4.27MNIDD96 pKa = 3.78EE97 pKa = 5.88LNDD100 pKa = 3.45TDD102 pKa = 4.64EE103 pKa = 4.85EE104 pKa = 4.43EE105 pKa = 4.19LTFYY109 pKa = 11.27LSLLMVLDD117 pKa = 3.71NHH119 pKa = 6.26QQ120 pKa = 3.28

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A078H8J7|A0A078H8J7_BRANA Aminomethyltransferase OS=Brassica napus OX=3708 GN=BnaA09g47510D PE=3 SV=1
MM1 pKa = 7.78KK2 pKa = 10.28KK3 pKa = 10.13RR4 pKa = 11.84LEE6 pKa = 4.02GNMMKK11 pKa = 10.43KK12 pKa = 9.61KK13 pKa = 10.38VKK15 pKa = 9.91RR16 pKa = 11.84KK17 pKa = 8.91RR18 pKa = 11.84LKK20 pKa = 10.03PVTKK24 pKa = 9.88RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84TVRR30 pKa = 11.84MMRR33 pKa = 11.84KK34 pKa = 9.31LKK36 pKa = 10.53KK37 pKa = 9.86KK38 pKa = 8.16WWNLRR43 pKa = 11.84LKK45 pKa = 10.93AKK47 pKa = 9.57MIKK50 pKa = 9.58QRR52 pKa = 11.84LKK54 pKa = 10.55GKK56 pKa = 10.04RR57 pKa = 11.84IKK59 pKa = 10.29KK60 pKa = 9.75KK61 pKa = 9.56KK62 pKa = 10.52LKK64 pKa = 10.54GKK66 pKa = 7.82SLKK69 pKa = 10.25PGSKK73 pKa = 9.44KK74 pKa = 9.6RR75 pKa = 11.84RR76 pKa = 11.84KK77 pKa = 8.87VRR79 pKa = 11.84LMRR82 pKa = 11.84WNLRR86 pKa = 11.84LGRR89 pKa = 11.84QRR91 pKa = 4.09

Molecular weight: 11.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60180

0

60180

19704506

10

5076

327.4

36.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.45 ± 0.009

1.88 ± 0.005

5.295 ± 0.008

6.614 ± 0.014

4.296 ± 0.007

6.452 ± 0.014

2.323 ± 0.005

5.074 ± 0.007

6.256 ± 0.011

9.544 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.004

4.265 ± 0.007

4.901 ± 0.011

3.453 ± 0.007

5.52 ± 0.008

9.07 ± 0.012

5.195 ± 0.006

6.785 ± 0.009

1.278 ± 0.003

2.812 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski