Amino acid dipepetide frequency for Brassica napus (Rape)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.017AlaAla: 6.017 ± 0.028	1.177AlaCys: 1.177 ± 0.009	2.911AlaAsp: 2.911 ± 0.014	4.099AlaGlu: 4.099 ± 0.018	2.688AlaPhe: 2.688 ± 0.013	3.867AlaGly: 3.867 ± 0.017	1.222AlaHis: 1.222 ± 0.007	3.346AlaIle: 3.346 ± 0.015	3.922AlaLys: 3.922 ± 0.016	6.178AlaLeu: 6.178 ± 0.023	1.8AlaMet: 1.8 ± 0.01	2.428AlaAsn: 2.428 ± 0.009	2.717AlaPro: 2.717 ± 0.015	1.913AlaGln: 1.913 ± 0.012	3.345AlaArg: 3.345 ± 0.015	5.961AlaSer: 5.961 ± 0.019	3.677AlaThr: 3.677 ± 0.015	4.789AlaVal: 4.789 ± 0.019	0.723AlaTrp: 0.723 ± 0.007	1.718AlaTyr: 1.718 ± 0.01	0.0AlaXaa: 0.0 ± 0.0
Cys	0.911CysAla: 0.911 ± 0.007	0.569CysCys: 0.569 ± 0.007	0.92CysAsp: 0.92 ± 0.008	0.905CysGlu: 0.905 ± 0.007	1.021CysPhe: 1.021 ± 0.008	1.451CysGly: 1.451 ± 0.014	0.441CysHis: 0.441 ± 0.005	0.96CysIle: 0.96 ± 0.007	1.136CysLys: 1.136 ± 0.009	1.977CysLeu: 1.977 ± 0.01	0.438CysMet: 0.438 ± 0.005	0.81CysAsn: 0.81 ± 0.007	0.904CysPro: 0.904 ± 0.008	0.536CysGln: 0.536 ± 0.005	1.073CysArg: 1.073 ± 0.008	1.807CysSer: 1.807 ± 0.012	0.849CysThr: 0.849 ± 0.007	1.254CysVal: 1.254 ± 0.01	0.24CysTrp: 0.24 ± 0.004	0.598CysTyr: 0.598 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	3.258AspAla: 3.258 ± 0.013	0.944AspCys: 0.944 ± 0.007	3.846AspAsp: 3.846 ± 0.021	4.165AspGlu: 4.165 ± 0.016	2.297AspPhe: 2.297 ± 0.011	3.707AspGly: 3.707 ± 0.016	1.322AspHis: 1.322 ± 0.009	2.77AspIle: 2.77 ± 0.012	2.836AspLys: 2.836 ± 0.016	5.086AspLeu: 5.086 ± 0.018	1.353AspMet: 1.353 ± 0.01	2.017AspAsn: 2.017 ± 0.01	2.633AspPro: 2.633 ± 0.012	1.77AspGln: 1.77 ± 0.011	2.322AspArg: 2.322 ± 0.012	4.247AspSer: 4.247 ± 0.017	2.282AspThr: 2.282 ± 0.011	3.844AspVal: 3.844 ± 0.013	0.692AspTrp: 0.692 ± 0.007	1.553AspTyr: 1.553 ± 0.009	0.0AspXaa: 0.0 ± 0.0
Glu	4.941GluAla: 4.941 ± 0.017	0.895GluCys: 0.895 ± 0.007	4.158GluAsp: 4.158 ± 0.017	7.066GluGlu: 7.066 ± 0.04	2.403GluPhe: 2.403 ± 0.013	3.542GluGly: 3.542 ± 0.016	1.212GluHis: 1.212 ± 0.008	3.742GluIle: 3.742 ± 0.014	4.947GluLys: 4.947 ± 0.022	5.678GluLeu: 5.678 ± 0.021	1.864GluMet: 1.864 ± 0.013	2.927GluAsn: 2.927 ± 0.014	2.227GluPro: 2.227 ± 0.012	2.0GluGln: 2.0 ± 0.011	3.542GluArg: 3.542 ± 0.016	4.804GluSer: 4.804 ± 0.021	3.686GluThr: 3.686 ± 0.014	4.136GluVal: 4.136 ± 0.014	0.747GluTrp: 0.747 ± 0.006	1.628GluTyr: 1.628 ± 0.009	0.0GluXaa: 0.0 ± 0.0
Phe	2.487PheAla: 2.487 ± 0.012	0.92PheCys: 0.92 ± 0.007	2.356PheAsp: 2.356 ± 0.011	2.303PheGlu: 2.303 ± 0.011	2.153PhePhe: 2.153 ± 0.013	3.06PheGly: 3.06 ± 0.016	1.105PheHis: 1.105 ± 0.007	2.091PheIle: 2.091 ± 0.012	2.274PheLys: 2.274 ± 0.012	4.454PheLeu: 4.454 ± 0.017	1.059PheMet: 1.059 ± 0.007	1.728PheAsn: 1.728 ± 0.01	2.09PhePro: 2.09 ± 0.01	1.474PheGln: 1.474 ± 0.009	2.154PheArg: 2.154 ± 0.011	4.169PheSer: 4.169 ± 0.014	2.261PheThr: 2.261 ± 0.013	2.936PheVal: 2.936 ± 0.013	0.583PheTrp: 0.583 ± 0.005	1.301PheTyr: 1.301 ± 0.009	0.0PheXaa: 0.0 ± 0.0
Gly	3.581GlyAla: 3.581 ± 0.015	1.291GlyCys: 1.291 ± 0.01	3.554GlyAsp: 3.554 ± 0.016	3.916GlyGlu: 3.916 ± 0.018	3.243GlyPhe: 3.243 ± 0.014	5.867GlyGly: 5.867 ± 0.061	1.434GlyHis: 1.434 ± 0.01	3.216GlyIle: 3.216 ± 0.016	4.127GlyLys: 4.127 ± 0.018	5.725GlyLeu: 5.725 ± 0.018	1.477GlyMet: 1.477 ± 0.009	2.91GlyAsn: 2.91 ± 0.014	2.306GlyPro: 2.306 ± 0.013	1.938GlyGln: 1.938 ± 0.011	3.582GlyArg: 3.582 ± 0.016	5.868GlySer: 5.868 ± 0.023	3.206GlyThr: 3.206 ± 0.013	4.317GlyVal: 4.317 ± 0.022	0.869GlyTrp: 0.869 ± 0.008	2.095GlyTyr: 2.095 ± 0.013	0.0GlyXaa: 0.0 ± 0.0
His	1.248HisAla: 1.248 ± 0.008	0.505HisCys: 0.505 ± 0.006	1.111HisAsp: 1.111 ± 0.008	1.281HisGlu: 1.281 ± 0.008	1.004HisPhe: 1.004 ± 0.007	1.658HisGly: 1.658 ± 0.01	1.034HisHis: 1.034 ± 0.012	1.159HisIle: 1.159 ± 0.007	1.211HisLys: 1.211 ± 0.009	2.325HisLeu: 2.325 ± 0.012	0.602HisMet: 0.602 ± 0.006	0.954HisAsn: 0.954 ± 0.007	1.28HisPro: 1.28 ± 0.008	1.053HisGln: 1.053 ± 0.009	1.415HisArg: 1.415 ± 0.009	1.83HisSer: 1.83 ± 0.01	0.971HisThr: 0.971 ± 0.007	1.601HisVal: 1.601 ± 0.009	0.302HisTrp: 0.302 ± 0.004	0.691HisTyr: 0.691 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	3.343IleAla: 3.343 ± 0.017	1.092IleCys: 1.092 ± 0.007	2.795IleAsp: 2.795 ± 0.014	2.979IleGlu: 2.979 ± 0.013	2.216IlePhe: 2.216 ± 0.011	3.227IleGly: 3.227 ± 0.013	1.263IleHis: 1.263 ± 0.008	2.65IleIle: 2.65 ± 0.013	2.911IleLys: 2.911 ± 0.012	4.851IleLeu: 4.851 ± 0.016	1.154IleMet: 1.154 ± 0.008	2.125IleAsn: 2.125 ± 0.013	2.722IlePro: 2.722 ± 0.015	1.83IleGln: 1.83 ± 0.01	2.619IleArg: 2.619 ± 0.012	4.651IleSer: 4.651 ± 0.017	2.706IleThr: 2.706 ± 0.014	3.424IleVal: 3.424 ± 0.015	0.676IleTrp: 0.676 ± 0.006	1.511IleTyr: 1.511 ± 0.009	0.0IleXaa: 0.0 ± 0.0
Lys	4.061LysAla: 4.061 ± 0.015	0.977LysCys: 0.977 ± 0.008	3.202LysAsp: 3.202 ± 0.015	4.767LysGlu: 4.767 ± 0.022	2.103LysPhe: 2.103 ± 0.011	3.52LysGly: 3.52 ± 0.014	1.362LysHis: 1.362 ± 0.009	3.271LysIle: 3.271 ± 0.015	5.364LysLys: 5.364 ± 0.025	6.072LysLeu: 6.072 ± 0.021	1.588LysMet: 1.588 ± 0.009	2.639LysAsn: 2.639 ± 0.012	3.029LysPro: 3.029 ± 0.016	2.234LysGln: 2.234 ± 0.012	3.975LysArg: 3.975 ± 0.016	4.737LysSer: 4.737 ± 0.02	3.425LysThr: 3.425 ± 0.015	3.846LysVal: 3.846 ± 0.015	0.804LysTrp: 0.804 ± 0.007	1.588LysTyr: 1.588 ± 0.009	0.0LysXaa: 0.0 ± 0.0
Leu	6.188LeuAla: 6.188 ± 0.019	1.934LeuCys: 1.934 ± 0.011	4.871LeuAsp: 4.871 ± 0.019	6.066LeuGlu: 6.066 ± 0.023	3.98LeuPhe: 3.98 ± 0.015	5.578LeuGly: 5.578 ± 0.018	2.422LeuHis: 2.422 ± 0.011	4.541LeuIle: 4.541 ± 0.016	6.095LeuLys: 6.095 ± 0.023	9.722LeuLeu: 9.722 ± 0.028	2.303LeuMet: 2.303 ± 0.01	3.776LeuAsn: 3.776 ± 0.015	5.036LeuPro: 5.036 ± 0.018	3.78LeuGln: 3.78 ± 0.016	5.526LeuArg: 5.526 ± 0.02	8.593LeuSer: 8.593 ± 0.027	4.786LeuThr: 4.786 ± 0.02	6.57LeuVal: 6.57 ± 0.021	1.186LeuTrp: 1.186 ± 0.008	2.485LeuTyr: 2.485 ± 0.011	0.0LeuXaa: 0.0 ± 0.0
Met	2.057MetAla: 2.057 ± 0.011	0.376MetCys: 0.376 ± 0.005	1.448MetAsp: 1.448 ± 0.01	2.022MetGlu: 2.022 ± 0.01	1.014MetPhe: 1.014 ± 0.007	1.573MetGly: 1.573 ± 0.009	0.486MetHis: 0.486 ± 0.005	1.339MetIle: 1.339 ± 0.009	1.755MetLys: 1.755 ± 0.01	2.171MetLeu: 2.171 ± 0.011	0.826MetMet: 0.826 ± 0.007	1.09MetAsn: 1.09 ± 0.007	0.949MetPro: 0.949 ± 0.007	0.804MetGln: 0.804 ± 0.007	1.304MetArg: 1.304 ± 0.008	2.036MetSer: 2.036 ± 0.01	1.161MetThr: 1.161 ± 0.008	1.815MetVal: 1.815 ± 0.011	0.281MetTrp: 0.281 ± 0.004	0.647MetTyr: 0.647 ± 0.006	0.0MetXaa: 0.0 ± 0.0
Asn	2.524AsnAla: 2.524 ± 0.011	0.779AsnCys: 0.779 ± 0.009	1.996AsnAsp: 1.996 ± 0.009	2.376AsnGlu: 2.376 ± 0.011	1.695AsnPhe: 1.695 ± 0.01	3.14AsnGly: 3.14 ± 0.017	1.159AsnHis: 1.159 ± 0.008	2.309AsnIle: 2.309 ± 0.013	2.497AsnLys: 2.497 ± 0.013	4.283AsnLeu: 4.283 ± 0.02	1.113AsnMet: 1.113 ± 0.008	2.411AsnAsn: 2.411 ± 0.018	2.359AsnPro: 2.359 ± 0.012	1.696AsnGln: 1.696 ± 0.01	2.14AsnArg: 2.14 ± 0.01	3.471AsnSer: 3.471 ± 0.014	2.096AsnThr: 2.096 ± 0.013	2.848AsnVal: 2.848 ± 0.012	0.525AsnTrp: 0.525 ± 0.005	1.23AsnTyr: 1.23 ± 0.008	0.0AsnXaa: 0.0 ± 0.0
Pro	2.871ProAla: 2.871 ± 0.014	0.79ProCys: 0.79 ± 0.007	2.394ProAsp: 2.394 ± 0.013	3.295ProGlu: 3.295 ± 0.013	1.997ProPhe: 1.997 ± 0.01	2.676ProGly: 2.676 ± 0.014	1.08ProHis: 1.08 ± 0.008	2.114ProIle: 2.114 ± 0.011	2.78ProLys: 2.78 ± 0.015	4.295ProLeu: 4.295 ± 0.018	1.019ProMet: 1.019 ± 0.008	2.158ProAsn: 2.158 ± 0.01	4.174ProPro: 4.174 ± 0.046	1.79ProGln: 1.79 ± 0.011	2.592ProArg: 2.592 ± 0.014	5.142ProSer: 5.142 ± 0.021	2.628ProThr: 2.628 ± 0.014	3.264ProVal: 3.264 ± 0.016	0.614ProTrp: 0.614 ± 0.006	1.332ProTyr: 1.332 ± 0.009	0.0ProXaa: 0.0 ± 0.0
Gln	2.244GlnAla: 2.244 ± 0.012	0.557GlnCys: 0.557 ± 0.005	1.614GlnAsp: 1.614 ± 0.008	2.45GlnGlu: 2.45 ± 0.013	1.284GlnPhe: 1.284 ± 0.008	2.033GlnGly: 2.033 ± 0.012	0.836GlnHis: 0.836 ± 0.007	1.838GlnIle: 1.838 ± 0.011	2.111GlnLys: 2.111 ± 0.012	3.113GlnLeu: 3.113 ± 0.014	0.934GlnMet: 0.934 ± 0.007	1.573GlnAsn: 1.573 ± 0.01	1.686GlnPro: 1.686 ± 0.012	1.922GlnGln: 1.922 ± 0.021	2.161GlnArg: 2.161 ± 0.013	2.703GlnSer: 2.703 ± 0.013	1.914GlnThr: 1.914 ± 0.011	2.245GlnVal: 2.245 ± 0.011	0.447GlnTrp: 0.447 ± 0.005	0.868GlnTyr: 0.868 ± 0.007	0.0GlnXaa: 0.0 ± 0.0
Arg	3.103ArgAla: 3.103 ± 0.014	1.098ArgCys: 1.098 ± 0.009	2.804ArgAsp: 2.804 ± 0.013	3.522ArgGlu: 3.522 ± 0.015	2.563ArgPhe: 2.563 ± 0.012	3.377ArgGly: 3.377 ± 0.017	1.274ArgHis: 1.274 ± 0.008	2.804ArgIle: 2.804 ± 0.014	3.849ArgLys: 3.849 ± 0.018	5.195ArgLeu: 5.195 ± 0.017	1.351ArgMet: 1.351 ± 0.008	2.454ArgAsn: 2.454 ± 0.014	2.335ArgPro: 2.335 ± 0.013	1.828ArgGln: 1.828 ± 0.01	4.383ArgArg: 4.383 ± 0.022	4.764ArgSer: 4.764 ± 0.017	2.583ArgThr: 2.583 ± 0.012	3.631ArgVal: 3.631 ± 0.013	0.764ArgTrp: 0.764 ± 0.006	1.512ArgTyr: 1.512 ± 0.01	0.0ArgXaa: 0.0 ± 0.0
Ser	5.058SerAla: 5.058 ± 0.018	1.752SerCys: 1.752 ± 0.011	4.551SerAsp: 4.551 ± 0.016	4.876SerGlu: 4.876 ± 0.021	4.205SerPhe: 4.205 ± 0.016	5.948SerGly: 5.948 ± 0.023	2.039SerHis: 2.039 ± 0.011	4.153SerIle: 4.153 ± 0.014	4.969SerLys: 4.969 ± 0.02	9.059SerLeu: 9.059 ± 0.028	2.092SerMet: 2.092 ± 0.01	3.794SerAsn: 3.794 ± 0.015	4.878SerPro: 4.878 ± 0.025	2.921SerGln: 2.921 ± 0.014	4.691SerArg: 4.691 ± 0.016	11.86SerSer: 11.86 ± 0.048	4.59SerThr: 4.59 ± 0.017	5.658SerVal: 5.658 ± 0.018	1.147SerTrp: 1.147 ± 0.008	2.463SerTyr: 2.463 ± 0.013	0.0SerXaa: 0.0 ± 0.0
Thr	3.424ThrAla: 3.424 ± 0.014	1.041ThrCys: 1.041 ± 0.008	2.392ThrAsp: 2.392 ± 0.013	3.09ThrGlu: 3.09 ± 0.014	2.171ThrPhe: 2.171 ± 0.011	3.463ThrGly: 3.463 ± 0.016	1.097ThrHis: 1.097 ± 0.007	2.752ThrIle: 2.752 ± 0.012	3.053ThrLys: 3.053 ± 0.016	4.807ThrLeu: 4.807 ± 0.018	1.301ThrMet: 1.301 ± 0.008	2.202ThrAsn: 2.202 ± 0.012	2.724ThrPro: 2.724 ± 0.013	1.624ThrGln: 1.624 ± 0.01	2.714ThrArg: 2.714 ± 0.013	4.82ThrSer: 4.82 ± 0.016	3.422ThrThr: 3.422 ± 0.017	3.729ThrVal: 3.729 ± 0.017	0.696ThrTrp: 0.696 ± 0.007	1.425ThrTyr: 1.425 ± 0.01	0.0ThrXaa: 0.0 ± 0.0
Val	4.788ValAla: 4.788 ± 0.019	1.245ValCys: 1.245 ± 0.01	3.774ValAsp: 3.774 ± 0.015	4.565ValGlu: 4.565 ± 0.021	2.992ValPhe: 2.992 ± 0.014	3.986ValGly: 3.986 ± 0.015	1.479ValHis: 1.479 ± 0.008	3.467ValIle: 3.467 ± 0.014	4.218ValLys: 4.218 ± 0.016	6.448ValLeu: 6.448 ± 0.018	1.747ValMet: 1.747 ± 0.01	2.692ValAsn: 2.692 ± 0.011	3.226ValPro: 3.226 ± 0.011	2.123ValGln: 2.123 ± 0.011	3.266ValArg: 3.266 ± 0.012	5.965ValSer: 5.965 ± 0.02	3.672ValThr: 3.672 ± 0.014	5.296ValVal: 5.296 ± 0.019	0.834ValTrp: 0.834 ± 0.007	2.066ValTyr: 2.066 ± 0.011	0.0ValXaa: 0.0 ± 0.0
Trp	0.689TrpAla: 0.689 ± 0.006	0.259TrpCys: 0.259 ± 0.004	0.699TrpAsp: 0.699 ± 0.006	0.788TrpGlu: 0.788 ± 0.007	0.603TrpPhe: 0.603 ± 0.005	0.722TrpGly: 0.722 ± 0.007	0.253TrpHis: 0.253 ± 0.003	0.735TrpIle: 0.735 ± 0.006	0.918TrpLys: 0.918 ± 0.009	1.22TrpLeu: 1.22 ± 0.009	0.342TrpMet: 0.342 ± 0.005	0.67TrpAsn: 0.67 ± 0.006	0.466TrpPro: 0.466 ± 0.005	0.389TrpGln: 0.389 ± 0.004	0.922TrpArg: 0.922 ± 0.007	1.045TrpSer: 1.045 ± 0.009	0.655TrpThr: 0.655 ± 0.005	0.798TrpVal: 0.798 ± 0.006	0.246TrpTrp: 0.246 ± 0.004	0.363TrpTyr: 0.363 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.707TyrAla: 1.707 ± 0.01	0.599TyrCys: 0.599 ± 0.006	1.549TyrAsp: 1.549 ± 0.01	1.613TyrGlu: 1.613 ± 0.01	1.328TyrPhe: 1.328 ± 0.008	2.128TyrGly: 2.128 ± 0.014	0.713TyrHis: 0.713 ± 0.007	1.484TyrIle: 1.484 ± 0.009	1.59TyrLys: 1.59 ± 0.009	2.777TyrLeu: 2.777 ± 0.013	0.793TyrMet: 0.793 ± 0.006	1.291TyrAsn: 1.291 ± 0.009	1.265TyrPro: 1.265 ± 0.008	0.906TyrGln: 0.906 ± 0.008	1.469TyrArg: 1.469 ± 0.01	2.233TyrSer: 2.233 ± 0.013	1.378TyrThr: 1.378 ± 0.008	1.846TyrVal: 1.846 ± 0.011	0.405TyrTrp: 0.405 ± 0.005	1.042TyrTyr: 1.042 ± 0.009	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.216XaaXaa: 0.216 ± 0.074

Statistics based on 60180 proteins (19704506 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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