Hyunsoonleella jejuensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Hyunsoonleella

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2939 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1H9B3U9|A0A1H9B3U9_9FLAO Exo-beta-1 3-glucanase GH17 family OS=Hyunsoonleella jejuensis OX=419940 GN=SAMN05421824_0444 PE=4 SV=1
MM1 pKa = 7.08MKK3 pKa = 10.45KK4 pKa = 8.36ITLLLLTFLSFSLGFSQTEE23 pKa = 3.99LLNLSFDD30 pKa = 3.92AAGSEE35 pKa = 4.95SVWTPIADD43 pKa = 3.63AASNPSEE50 pKa = 4.06VNIAFDD56 pKa = 4.51ANGNPTGATLLSGINTSDD74 pKa = 3.28VAGRR78 pKa = 11.84AYY80 pKa = 9.75IFRR83 pKa = 11.84YY84 pKa = 10.17NNASFDD90 pKa = 3.81FGTSGSVSISMDD102 pKa = 3.32VKK104 pKa = 10.49IDD106 pKa = 3.45AALTGTNLVFEE117 pKa = 4.58TQVSKK122 pKa = 11.35VGGGVVVVTHH132 pKa = 6.56NNVQNDD138 pKa = 3.68VAVGGGWIPLTFDD151 pKa = 3.19MTPNPAEE158 pKa = 4.04FNNAGTEE165 pKa = 4.17LYY167 pKa = 10.45FYY169 pKa = 11.23FNMAAGAFVGAGGTFLVDD187 pKa = 3.79NIVVTGGAPAEE198 pKa = 4.17PTCDD202 pKa = 3.58DD203 pKa = 3.44GLMNGDD209 pKa = 3.92EE210 pKa = 4.38TGVDD214 pKa = 4.11CGGPDD219 pKa = 3.58CSACIEE225 pKa = 4.91DD226 pKa = 4.17PTDD229 pKa = 3.83APSEE233 pKa = 4.01LASTGTDD240 pKa = 3.26LYY242 pKa = 11.05AYY244 pKa = 9.88SGLSAVGEE252 pKa = 4.07SDD254 pKa = 4.25LPGFNFTAFNGGVTISEE271 pKa = 4.3EE272 pKa = 4.16TLSGNKK278 pKa = 9.17VGKK281 pKa = 9.4LANLDD286 pKa = 3.83FFGSGWTPIDD296 pKa = 3.2VTTAYY301 pKa = 9.64TYY303 pKa = 11.38VHH305 pKa = 7.04LDD307 pKa = 3.34YY308 pKa = 11.13YY309 pKa = 10.94AVSGTWFEE317 pKa = 4.67FFLIDD322 pKa = 4.73DD323 pKa = 4.38SLSATICCGSGEE335 pKa = 4.1EE336 pKa = 3.88PRR338 pKa = 11.84YY339 pKa = 10.28RR340 pKa = 11.84FGPAPSGQDD349 pKa = 3.13EE350 pKa = 4.41PLVLGAWTSVFIPLSDD366 pKa = 3.94FANYY370 pKa = 9.63AALTNGTWDD379 pKa = 3.41GTDD382 pKa = 2.81IKK384 pKa = 9.51EE385 pKa = 4.57TKK387 pKa = 8.89FTSDD391 pKa = 2.4GGTIYY396 pKa = 10.65FDD398 pKa = 3.97NIYY401 pKa = 10.49FSTSNVLGVDD411 pKa = 3.83DD412 pKa = 5.26FEE414 pKa = 4.61STSFSVYY421 pKa = 10.28PNPTQEE427 pKa = 3.43SWTIKK432 pKa = 10.09AQNTKK437 pKa = 8.11ITNIKK442 pKa = 10.18VYY444 pKa = 10.67DD445 pKa = 3.78VLGKK449 pKa = 10.14NVMMLSPNTIEE460 pKa = 4.17TKK462 pKa = 10.11IDD464 pKa = 3.31GASLMKK470 pKa = 10.58GIYY473 pKa = 8.37FAKK476 pKa = 10.07IEE478 pKa = 4.47TVSGTSSVKK487 pKa = 10.21LVKK490 pKa = 10.26QQ491 pKa = 3.39

Molecular weight:
51.97 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1H9BXK1|A0A1H9BXK1_9FLAO Magnesium transporter MgtE OS=Hyunsoonleella jejuensis OX=419940 GN=SAMN05421824_0742 PE=3 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.21KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.62KK19 pKa = 9.04RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.77KK27 pKa = 10.58KK28 pKa = 9.8KK29 pKa = 10.51KK30 pKa = 10.04

Molecular weight:
3.76 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2939

0

2939

1056956

30

3421

359.6

40.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.421 ± 0.04

0.804 ± 0.015

5.809 ± 0.036

6.491 ± 0.048

5.224 ± 0.036

6.418 ± 0.047

1.817 ± 0.022

7.893 ± 0.042

7.766 ± 0.072

9.091 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.109 ± 0.024

6.368 ± 0.048

3.421 ± 0.027

3.194 ± 0.022

3.457 ± 0.031

6.375 ± 0.041

5.862 ± 0.055

6.326 ± 0.033

1.079 ± 0.02

4.076 ± 0.025

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski