Isoelectric point predictions for nr (NCBI) database 
(406M protein sequences)

.
|-- nr_flat.00.IPC2.fasta
|-- ...
|-- nr_flat.24.IPC2.fasta
|-- README.txt


Due to the size of the whole database the orginal file has been
divided into the smaller files with 16.5M sequences each.

You can marge the files by 'cat', for instance: 
cat nr_flat.??.IPC2.fasta > nr.IPC2.fasta

Note: Complete file is 232GB thus before downloading and marging
make sure that you have at least 0.5TB of free disc space

===========================================================================================

Alternative mirror of the files:
http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/

Should also work:

mkdir nr/;cd nr;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/README.txt;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.00.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.01.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.02.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.03.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.04.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.05.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.06.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.07.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.08.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.09.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.10.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.11.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.12.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.13.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.14.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.15.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.16.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.17.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.18.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.19.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.20.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.21.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.22.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.23.IPC2.fasta.7z;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.24.IPC2.fasta.7z;

for file in `ls *fasta.7z`; do 7z e $file ; done;
cat nr_flat.??.IPC2.fasta > nr.IPC2.fasta;

# or as single file
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/uniprot/nr_flat.IPC2.fasta.7z;
7z e nr_flat.IPC2.fasta.7z;

#alternatively, compressed with zip
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.zip;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z01;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z02;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z03;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z04;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z05;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z06;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z07;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z08;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z09;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z10;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z11;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z12;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z13;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z14;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z15;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z16;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z17;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z18;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z19;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z20;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z21;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z22;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z23;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z24;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z25;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z26;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z27;
wget http://isoelectricpointdb2.mimuw.edu.pl/extra_large_db/nr/nr_flat.IPC2.fasta.z28;

7z e nr_flat.IPC2.fasta.zip;

===========================================================================================

Contains predictions: Bjellqvist,DTASelect,Dawson,EMBOSS,Grimsley,
IPC2_peptide,IPC2_protein,IPC_peptide,IPC_protein,Lehninger,Nozaki,
Patrickios,ProMoST,Rodwell,Sillero,Solomon,Thurlkill,Toseland,Wikipedia

===========================================================================================

References:

Kozlowski LP (2022) Proteome-pI 2.0: proteome isoelectric point database update.
Nucleic Acids Res. (Database Issue) 50 (D1): D1535-D1540, doi: 10.1093/nar/gkab944

Kozlowski LP (2021) IPC 2.0 - prediction of isoelectric point and pKa dissociation 
constants. Nucleic Acids Res. 49 (W1): W285-W292. doi: 10.1093/nar/gkab295

__author__ = "Lukasz Pawel Kozlowski"
__email__  = "lukaszkozlowski.lpk@gmail.com"
__copyrights__ = "Lukasz Pawel Kozlowski"
__website__ = "http://isoelectricpointdb2.org"