Amino acid dipepetide frequency for Pseudomonas phage UNO-SLW2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.294AlaAla: 11.294 ± 1.368
0.655AlaCys: 0.655 ± 0.265
6.138AlaAsp: 6.138 ± 0.945
6.22AlaGlu: 6.22 ± 0.614
3.437AlaPhe: 3.437 ± 0.572
7.775AlaGly: 7.775 ± 0.845
2.21AlaHis: 2.21 ± 0.502
3.601AlaIle: 3.601 ± 0.594
6.302AlaLys: 6.302 ± 0.572
8.757AlaLeu: 8.757 ± 1.115
3.519AlaMet: 3.519 ± 0.636
4.092AlaAsn: 4.092 ± 0.838
3.437AlaPro: 3.437 ± 0.607
5.729AlaGln: 5.729 ± 1.166
6.547AlaArg: 6.547 ± 0.769
7.12AlaSer: 7.12 ± 0.968
4.829AlaThr: 4.829 ± 1.233
6.547AlaVal: 6.547 ± 0.605
1.309AlaTrp: 1.309 ± 0.401
3.192AlaTyr: 3.192 ± 0.57
0.0AlaXaa: 0.0 ± 0.0
Cys
1.146CysAla: 1.146 ± 0.311
0.0CysCys: 0.0 ± 0.0
0.573CysAsp: 0.573 ± 0.304
0.573CysGlu: 0.573 ± 0.191
0.409CysPhe: 0.409 ± 0.235
0.818CysGly: 0.818 ± 0.277
0.164CysHis: 0.164 ± 0.109
0.246CysIle: 0.246 ± 0.14
0.491CysLys: 0.491 ± 0.269
1.064CysLeu: 1.064 ± 0.328
0.246CysMet: 0.246 ± 0.135
0.082CysAsn: 0.082 ± 0.082
0.164CysPro: 0.164 ± 0.121
0.409CysGln: 0.409 ± 0.205
0.573CysArg: 0.573 ± 0.22
0.327CysSer: 0.327 ± 0.145
0.327CysThr: 0.327 ± 0.172
0.327CysVal: 0.327 ± 0.164
0.164CysTrp: 0.164 ± 0.122
0.246CysTyr: 0.246 ± 0.163
0.0CysXaa: 0.0 ± 0.0
Asp
6.465AspAla: 6.465 ± 0.686
0.409AspCys: 0.409 ± 0.177
4.665AspAsp: 4.665 ± 0.659
3.519AspGlu: 3.519 ± 0.573
3.11AspPhe: 3.11 ± 0.435
6.793AspGly: 6.793 ± 0.773
1.473AspHis: 1.473 ± 0.387
3.437AspIle: 3.437 ± 0.397
3.192AspLys: 3.192 ± 0.462
4.174AspLeu: 4.174 ± 0.708
1.882AspMet: 1.882 ± 0.425
1.473AspAsn: 1.473 ± 0.296
3.192AspPro: 3.192 ± 0.702
2.373AspGln: 2.373 ± 0.388
3.846AspArg: 3.846 ± 0.728
3.028AspSer: 3.028 ± 0.711
3.11AspThr: 3.11 ± 0.55
3.846AspVal: 3.846 ± 0.583
1.228AspTrp: 1.228 ± 0.378
1.719AspTyr: 1.719 ± 0.23
0.0AspXaa: 0.0 ± 0.0
Glu
8.511GluAla: 8.511 ± 0.9
0.818GluCys: 0.818 ± 0.333
4.174GluAsp: 4.174 ± 0.46
5.238GluGlu: 5.238 ± 1.139
2.455GluPhe: 2.455 ± 0.426
5.156GluGly: 5.156 ± 0.742
1.555GluHis: 1.555 ± 0.358
2.946GluIle: 2.946 ± 0.451
3.355GluLys: 3.355 ± 0.499
5.811GluLeu: 5.811 ± 0.733
1.964GluMet: 1.964 ± 0.521
2.783GluAsn: 2.783 ± 0.391
2.128GluPro: 2.128 ± 0.434
2.292GluGln: 2.292 ± 0.38
3.928GluArg: 3.928 ± 0.556
3.519GluSer: 3.519 ± 0.551
4.419GluThr: 4.419 ± 0.541
4.174GluVal: 4.174 ± 0.593
1.391GluTrp: 1.391 ± 0.432
2.128GluTyr: 2.128 ± 0.363
0.0GluXaa: 0.0 ± 0.0
Phe
2.046PheAla: 2.046 ± 0.569
0.491PheCys: 0.491 ± 0.279
3.028PheAsp: 3.028 ± 0.618
2.128PheGlu: 2.128 ± 0.409
1.391PhePhe: 1.391 ± 0.309
2.946PheGly: 2.946 ± 0.413
0.818PheHis: 0.818 ± 0.225
1.309PheIle: 1.309 ± 0.408
2.128PheLys: 2.128 ± 0.394
3.683PheLeu: 3.683 ± 0.607
1.309PheMet: 1.309 ± 0.239
1.882PheAsn: 1.882 ± 0.432
1.555PhePro: 1.555 ± 0.42
0.737PheGln: 0.737 ± 0.237
2.373PheArg: 2.373 ± 0.378
1.637PheSer: 1.637 ± 0.4
2.537PheThr: 2.537 ± 0.378
2.128PheVal: 2.128 ± 0.41
0.573PheTrp: 0.573 ± 0.191
0.818PheTyr: 0.818 ± 0.386
0.0PheXaa: 0.0 ± 0.0
Gly
7.366GlyAla: 7.366 ± 1.097
0.9GlyCys: 0.9 ± 0.263
4.91GlyAsp: 4.91 ± 0.545
5.565GlyGlu: 5.565 ± 0.636
3.765GlyPhe: 3.765 ± 0.617
7.12GlyGly: 7.12 ± 0.838
1.637GlyHis: 1.637 ± 0.276
4.501GlyIle: 4.501 ± 0.841
4.501GlyLys: 4.501 ± 0.896
6.956GlyLeu: 6.956 ± 0.856
2.046GlyMet: 2.046 ± 0.444
3.028GlyAsn: 3.028 ± 0.489
2.537GlyPro: 2.537 ± 0.504
2.537GlyGln: 2.537 ± 0.453
5.483GlyArg: 5.483 ± 0.677
5.401GlySer: 5.401 ± 0.552
4.092GlyThr: 4.092 ± 0.512
5.401GlyVal: 5.401 ± 0.612
1.473GlyTrp: 1.473 ± 0.353
3.11GlyTyr: 3.11 ± 0.543
0.0GlyXaa: 0.0 ± 0.0
His
1.555HisAla: 1.555 ± 0.388
0.409HisCys: 0.409 ± 0.199
1.228HisAsp: 1.228 ± 0.446
1.719HisGlu: 1.719 ± 0.424
0.9HisPhe: 0.9 ± 0.314
1.882HisGly: 1.882 ± 0.43
0.491HisHis: 0.491 ± 0.245
0.9HisIle: 0.9 ± 0.27
1.309HisLys: 1.309 ± 0.31
2.292HisLeu: 2.292 ± 0.412
0.491HisMet: 0.491 ± 0.184
0.982HisAsn: 0.982 ± 0.297
0.737HisPro: 0.737 ± 0.232
0.737HisGln: 0.737 ± 0.198
1.391HisArg: 1.391 ± 0.331
1.064HisSer: 1.064 ± 0.241
1.146HisThr: 1.146 ± 0.263
1.637HisVal: 1.637 ± 0.415
0.655HisTrp: 0.655 ± 0.21
0.9HisTyr: 0.9 ± 0.24
0.0HisXaa: 0.0 ± 0.0
Ile
3.437IleAla: 3.437 ± 0.544
0.573IleCys: 0.573 ± 0.226
2.946IleAsp: 2.946 ± 0.526
3.683IleGlu: 3.683 ± 0.563
0.9IlePhe: 0.9 ± 0.282
3.192IleGly: 3.192 ± 0.537
0.9IleHis: 0.9 ± 0.331
2.292IleIle: 2.292 ± 0.432
2.864IleLys: 2.864 ± 0.621
3.846IleLeu: 3.846 ± 0.498
1.391IleMet: 1.391 ± 0.343
1.309IleAsn: 1.309 ± 0.356
2.455IlePro: 2.455 ± 0.449
2.128IleGln: 2.128 ± 0.484
3.192IleArg: 3.192 ± 0.305
1.882IleSer: 1.882 ± 0.28
2.783IleThr: 2.783 ± 0.425
3.274IleVal: 3.274 ± 0.485
0.327IleTrp: 0.327 ± 0.171
1.8IleTyr: 1.8 ± 0.595
0.0IleXaa: 0.0 ± 0.0
Lys
8.184LysAla: 8.184 ± 0.857
0.491LysCys: 0.491 ± 0.223
4.256LysAsp: 4.256 ± 0.546
4.01LysGlu: 4.01 ± 0.61
2.537LysPhe: 2.537 ± 0.396
4.91LysGly: 4.91 ± 0.696
1.719LysHis: 1.719 ± 0.524
2.373LysIle: 2.373 ± 0.376
3.192LysLys: 3.192 ± 0.776
4.992LysLeu: 4.992 ± 0.731
1.719LysMet: 1.719 ± 0.406
1.964LysAsn: 1.964 ± 0.468
2.537LysPro: 2.537 ± 0.514
2.21LysGln: 2.21 ± 0.452
3.355LysArg: 3.355 ± 0.645
2.537LysSer: 2.537 ± 0.399
3.519LysThr: 3.519 ± 0.603
3.601LysVal: 3.601 ± 0.661
0.573LysTrp: 0.573 ± 0.191
1.146LysTyr: 1.146 ± 0.253
0.0LysXaa: 0.0 ± 0.0
Leu
8.429LeuAla: 8.429 ± 1.003
0.164LeuCys: 0.164 ± 0.129
4.992LeuAsp: 4.992 ± 0.631
6.302LeuGlu: 6.302 ± 0.893
2.373LeuPhe: 2.373 ± 0.504
5.32LeuGly: 5.32 ± 0.767
2.046LeuHis: 2.046 ± 0.434
3.683LeuIle: 3.683 ± 0.552
7.12LeuLys: 7.12 ± 0.68
5.892LeuLeu: 5.892 ± 0.828
2.864LeuMet: 2.864 ± 0.475
3.846LeuAsn: 3.846 ± 0.465
2.864LeuPro: 2.864 ± 0.493
4.092LeuGln: 4.092 ± 0.472
4.501LeuArg: 4.501 ± 0.505
4.992LeuSer: 4.992 ± 0.669
5.156LeuThr: 5.156 ± 0.855
5.892LeuVal: 5.892 ± 0.621
1.064LeuTrp: 1.064 ± 0.303
1.882LeuTyr: 1.882 ± 0.425
0.0LeuXaa: 0.0 ± 0.0
Met
3.928MetAla: 3.928 ± 0.571
0.164MetCys: 0.164 ± 0.108
1.637MetAsp: 1.637 ± 0.458
2.537MetGlu: 2.537 ± 0.557
0.327MetPhe: 0.327 ± 0.165
2.537MetGly: 2.537 ± 0.431
0.573MetHis: 0.573 ± 0.192
1.391MetIle: 1.391 ± 0.26
1.146MetLys: 1.146 ± 0.35
2.701MetLeu: 2.701 ± 0.474
0.655MetMet: 0.655 ± 0.219
1.228MetAsn: 1.228 ± 0.303
1.391MetPro: 1.391 ± 0.35
0.818MetGln: 0.818 ± 0.236
1.309MetArg: 1.309 ± 0.232
2.21MetSer: 2.21 ± 0.37
2.128MetThr: 2.128 ± 0.414
2.046MetVal: 2.046 ± 0.343
0.327MetTrp: 0.327 ± 0.148
0.409MetTyr: 0.409 ± 0.157
0.0MetXaa: 0.0 ± 0.0
Asn
3.683AsnAla: 3.683 ± 0.541
0.164AsnCys: 0.164 ± 0.12
1.964AsnAsp: 1.964 ± 0.426
2.292AsnGlu: 2.292 ± 0.478
2.292AsnPhe: 2.292 ± 0.323
3.765AsnGly: 3.765 ± 0.589
0.9AsnHis: 0.9 ± 0.334
1.637AsnIle: 1.637 ± 0.471
1.637AsnLys: 1.637 ± 0.352
4.174AsnLeu: 4.174 ± 0.454
1.146AsnMet: 1.146 ± 0.262
1.064AsnAsn: 1.064 ± 0.297
2.783AsnPro: 2.783 ± 0.463
0.982AsnGln: 0.982 ± 0.255
2.455AsnArg: 2.455 ± 0.525
0.818AsnSer: 0.818 ± 0.355
2.455AsnThr: 2.455 ± 0.556
2.864AsnVal: 2.864 ± 0.414
0.246AsnTrp: 0.246 ± 0.167
1.555AsnTyr: 1.555 ± 0.462
0.0AsnXaa: 0.0 ± 0.0
Pro
2.946ProAla: 2.946 ± 0.471
0.246ProCys: 0.246 ± 0.149
3.928ProAsp: 3.928 ± 0.526
3.683ProGlu: 3.683 ± 0.606
1.473ProPhe: 1.473 ± 0.273
2.537ProGly: 2.537 ± 0.407
0.9ProHis: 0.9 ± 0.243
1.391ProIle: 1.391 ± 0.426
2.619ProLys: 2.619 ± 0.427
2.21ProLeu: 2.21 ± 0.394
0.9ProMet: 0.9 ± 0.237
2.292ProAsn: 2.292 ± 0.456
1.391ProPro: 1.391 ± 0.398
1.391ProGln: 1.391 ± 0.261
2.128ProArg: 2.128 ± 0.37
2.21ProSer: 2.21 ± 0.486
2.21ProThr: 2.21 ± 0.481
3.274ProVal: 3.274 ± 0.533
0.573ProTrp: 0.573 ± 0.227
0.9ProTyr: 0.9 ± 0.372
0.0ProXaa: 0.0 ± 0.0
Gln
5.729GlnAla: 5.729 ± 1.235
0.082GlnCys: 0.082 ± 0.079
2.373GlnAsp: 2.373 ± 0.524
2.21GlnGlu: 2.21 ± 0.436
2.128GlnPhe: 2.128 ± 0.485
3.519GlnGly: 3.519 ± 0.58
0.409GlnHis: 0.409 ± 0.192
2.21GlnIle: 2.21 ± 0.388
1.964GlnLys: 1.964 ± 0.577
3.928GlnLeu: 3.928 ± 0.471
1.555GlnMet: 1.555 ± 0.428
1.064GlnAsn: 1.064 ± 0.294
0.9GlnPro: 0.9 ± 0.267
1.8GlnGln: 1.8 ± 0.373
2.373GlnArg: 2.373 ± 0.568
1.8GlnSer: 1.8 ± 0.312
1.555GlnThr: 1.555 ± 0.338
2.783GlnVal: 2.783 ± 0.414
0.655GlnTrp: 0.655 ± 0.229
1.637GlnTyr: 1.637 ± 0.453
0.0GlnXaa: 0.0 ± 0.0
Arg
6.056ArgAla: 6.056 ± 0.764
0.573ArgCys: 0.573 ± 0.304
3.437ArgAsp: 3.437 ± 0.606
4.338ArgGlu: 4.338 ± 0.65
1.637ArgPhe: 1.637 ± 0.376
5.238ArgGly: 5.238 ± 0.617
1.473ArgHis: 1.473 ± 0.338
2.864ArgIle: 2.864 ± 0.414
3.192ArgLys: 3.192 ± 0.628
4.829ArgLeu: 4.829 ± 0.592
1.719ArgMet: 1.719 ± 0.452
3.274ArgAsn: 3.274 ± 0.584
2.455ArgPro: 2.455 ± 0.425
3.355ArgGln: 3.355 ± 0.615
3.437ArgArg: 3.437 ± 0.429
3.274ArgSer: 3.274 ± 0.493
2.373ArgThr: 2.373 ± 0.401
3.683ArgVal: 3.683 ± 0.518
0.655ArgTrp: 0.655 ± 0.229
1.964ArgTyr: 1.964 ± 0.286
0.0ArgXaa: 0.0 ± 0.0
Ser
5.32SerAla: 5.32 ± 0.876
0.655SerCys: 0.655 ± 0.234
3.437SerAsp: 3.437 ± 0.505
2.783SerGlu: 2.783 ± 0.406
2.21SerPhe: 2.21 ± 0.333
5.238SerGly: 5.238 ± 0.639
1.309SerHis: 1.309 ± 0.341
2.537SerIle: 2.537 ± 0.414
3.192SerLys: 3.192 ± 0.605
4.501SerLeu: 4.501 ± 0.66
1.473SerMet: 1.473 ± 0.394
2.128SerAsn: 2.128 ± 0.582
1.964SerPro: 1.964 ± 0.389
1.8SerGln: 1.8 ± 0.387
3.11SerArg: 3.11 ± 0.456
2.619SerSer: 2.619 ± 0.51
2.373SerThr: 2.373 ± 0.433
3.683SerVal: 3.683 ± 0.623
0.9SerTrp: 0.9 ± 0.218
1.964SerTyr: 1.964 ± 0.418
0.0SerXaa: 0.0 ± 0.0
Thr
4.992ThrAla: 4.992 ± 0.55
0.491ThrCys: 0.491 ± 0.206
3.028ThrAsp: 3.028 ± 0.601
4.829ThrGlu: 4.829 ± 0.636
1.473ThrPhe: 1.473 ± 0.522
5.156ThrGly: 5.156 ± 0.833
1.228ThrHis: 1.228 ± 0.278
3.274ThrIle: 3.274 ± 0.465
4.338ThrLys: 4.338 ± 0.614
5.483ThrLeu: 5.483 ± 0.567
1.146ThrMet: 1.146 ± 0.277
2.128ThrAsn: 2.128 ± 0.441
3.028ThrPro: 3.028 ± 0.516
2.455ThrGln: 2.455 ± 0.553
2.455ThrArg: 2.455 ± 0.599
2.537ThrSer: 2.537 ± 0.614
3.437ThrThr: 3.437 ± 0.639
3.683ThrVal: 3.683 ± 0.668
0.409ThrTrp: 0.409 ± 0.211
1.228ThrTyr: 1.228 ± 0.277
0.0ThrXaa: 0.0 ± 0.0
Val
6.956ValAla: 6.956 ± 0.842
0.409ValCys: 0.409 ± 0.169
3.437ValAsp: 3.437 ± 0.582
4.501ValGlu: 4.501 ± 0.638
1.637ValPhe: 1.637 ± 0.388
4.829ValGly: 4.829 ± 0.597
1.473ValHis: 1.473 ± 0.296
2.946ValIle: 2.946 ± 0.462
4.501ValLys: 4.501 ± 0.735
4.583ValLeu: 4.583 ± 0.624
1.473ValMet: 1.473 ± 0.38
2.701ValAsn: 2.701 ± 0.565
2.537ValPro: 2.537 ± 0.442
2.946ValGln: 2.946 ± 0.463
4.665ValArg: 4.665 ± 0.747
4.01ValSer: 4.01 ± 0.48
5.401ValThr: 5.401 ± 0.779
4.501ValVal: 4.501 ± 0.715
1.309ValTrp: 1.309 ± 0.384
1.964ValTyr: 1.964 ± 0.42
0.0ValXaa: 0.0 ± 0.0
Trp
1.555TrpAla: 1.555 ± 0.44
0.164TrpCys: 0.164 ± 0.129
0.818TrpAsp: 0.818 ± 0.23
0.573TrpGlu: 0.573 ± 0.202
0.491TrpPhe: 0.491 ± 0.212
0.982TrpGly: 0.982 ± 0.208
0.655TrpHis: 0.655 ± 0.205
0.573TrpIle: 0.573 ± 0.277
1.309TrpLys: 1.309 ± 0.362
1.719TrpLeu: 1.719 ± 0.38
0.491TrpMet: 0.491 ± 0.207
0.573TrpAsn: 0.573 ± 0.296
0.409TrpPro: 0.409 ± 0.205
0.573TrpGln: 0.573 ± 0.233
0.491TrpArg: 0.491 ± 0.185
1.064TrpSer: 1.064 ± 0.383
0.655TrpThr: 0.655 ± 0.23
1.309TrpVal: 1.309 ± 0.349
0.082TrpTrp: 0.082 ± 0.082
0.164TrpTyr: 0.164 ± 0.112
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.11TyrAla: 3.11 ± 0.666
0.491TyrCys: 0.491 ± 0.217
1.964TyrAsp: 1.964 ± 0.42
2.128TyrGlu: 2.128 ± 0.46
0.573TyrPhe: 0.573 ± 0.21
2.619TyrGly: 2.619 ± 0.403
0.491TyrHis: 0.491 ± 0.188
1.064TyrIle: 1.064 ± 0.233
1.473TyrLys: 1.473 ± 0.322
1.882TyrLeu: 1.882 ± 0.374
1.391TyrMet: 1.391 ± 0.395
0.982TyrAsn: 0.982 ± 0.285
0.737TyrPro: 0.737 ± 0.215
1.309TyrGln: 1.309 ± 0.35
2.21TyrArg: 2.21 ± 0.364
1.146TyrSer: 1.146 ± 0.317
2.292TyrThr: 2.292 ± 0.553
2.128TyrVal: 2.128 ± 0.432
0.737TyrTrp: 0.737 ± 0.218
0.327TyrTyr: 0.327 ± 0.183
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (12220 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski