Amino acid dipepetide frequency for Synechococcus phage ACG-2014f

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.083AlaAla: 4.083 ± 0.333
0.496AlaCys: 0.496 ± 0.093
3.223AlaAsp: 3.223 ± 0.26
3.412AlaGlu: 3.412 ± 0.268
2.435AlaPhe: 2.435 ± 0.209
4.739AlaGly: 4.739 ± 0.352
0.919AlaHis: 0.919 ± 0.134
3.587AlaIle: 3.587 ± 0.228
3.718AlaLys: 3.718 ± 0.338
4.535AlaLeu: 4.535 ± 0.363
1.414AlaMet: 1.414 ± 0.17
3.135AlaAsn: 3.135 ± 0.266
2.391AlaPro: 2.391 ± 0.245
2.319AlaGln: 2.319 ± 0.284
2.537AlaArg: 2.537 ± 0.186
4.608AlaSer: 4.608 ± 0.271
5.016AlaThr: 5.016 ± 0.773
3.412AlaVal: 3.412 ± 0.238
0.525AlaTrp: 0.525 ± 0.104
1.954AlaTyr: 1.954 ± 0.162
0.0AlaXaa: 0.0 ± 0.0
Cys
0.54CysAla: 0.54 ± 0.088
0.16CysCys: 0.16 ± 0.044
0.933CysAsp: 0.933 ± 0.165
0.554CysGlu: 0.554 ± 0.103
0.35CysPhe: 0.35 ± 0.082
0.627CysGly: 0.627 ± 0.111
0.19CysHis: 0.19 ± 0.063
0.598CysIle: 0.598 ± 0.093
0.481CysLys: 0.481 ± 0.083
0.758CysLeu: 0.758 ± 0.124
0.306CysMet: 0.306 ± 0.058
0.554CysAsn: 0.554 ± 0.093
0.496CysPro: 0.496 ± 0.109
0.19CysGln: 0.19 ± 0.054
0.394CysArg: 0.394 ± 0.086
0.598CysSer: 0.598 ± 0.091
0.496CysThr: 0.496 ± 0.082
0.671CysVal: 0.671 ± 0.107
0.131CysTrp: 0.131 ± 0.044
0.467CysTyr: 0.467 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
3.937AspAla: 3.937 ± 0.231
0.787AspCys: 0.787 ± 0.123
3.864AspAsp: 3.864 ± 0.218
4.098AspGlu: 4.098 ± 0.263
2.96AspPhe: 2.96 ± 0.194
5.235AspGly: 5.235 ± 0.373
0.846AspHis: 0.846 ± 0.114
4.666AspIle: 4.666 ± 0.328
3.631AspLys: 3.631 ± 0.318
5.556AspLeu: 5.556 ± 0.416
1.327AspMet: 1.327 ± 0.171
4.025AspAsn: 4.025 ± 0.281
2.8AspPro: 2.8 ± 0.193
1.969AspGln: 1.969 ± 0.169
2.654AspArg: 2.654 ± 0.186
4.229AspSer: 4.229 ± 0.293
4.404AspThr: 4.404 ± 0.262
5.133AspVal: 5.133 ± 0.337
1.035AspTrp: 1.035 ± 0.181
3.325AspTyr: 3.325 ± 0.294
0.0AspXaa: 0.0 ± 0.0
Glu
3.252GluAla: 3.252 ± 0.265
0.846GluCys: 0.846 ± 0.13
4.316GluAsp: 4.316 ± 0.302
5.658GluGlu: 5.658 ± 0.532
3.339GluPhe: 3.339 ± 0.266
4.302GluGly: 4.302 ± 0.342
1.079GluHis: 1.079 ± 0.131
3.893GluIle: 3.893 ± 0.28
3.85GluLys: 3.85 ± 0.368
5.366GluLeu: 5.366 ± 0.363
1.619GluMet: 1.619 ± 0.22
3.237GluAsn: 3.237 ± 0.236
2.085GluPro: 2.085 ± 0.24
1.954GluGln: 1.954 ± 0.205
2.829GluArg: 2.829 ± 0.2
4.141GluSer: 4.141 ± 0.329
4.098GluThr: 4.098 ± 0.274
5.206GluVal: 5.206 ± 0.293
0.802GluTrp: 0.802 ± 0.119
2.989GluTyr: 2.989 ± 0.245
0.0GluXaa: 0.0 ± 0.0
Phe
2.596PheAla: 2.596 ± 0.175
0.554PheCys: 0.554 ± 0.109
3.281PheAsp: 3.281 ± 0.224
2.537PheGlu: 2.537 ± 0.236
1.633PhePhe: 1.633 ± 0.168
3.193PheGly: 3.193 ± 0.209
0.7PheHis: 0.7 ± 0.118
2.741PheIle: 2.741 ± 0.153
2.085PheLys: 2.085 ± 0.211
3.179PheLeu: 3.179 ± 0.246
0.977PheMet: 0.977 ± 0.126
2.45PheAsn: 2.45 ± 0.201
2.042PhePro: 2.042 ± 0.394
1.473PheGln: 1.473 ± 0.125
2.027PheArg: 2.027 ± 0.17
3.368PheSer: 3.368 ± 0.196
3.675PheThr: 3.675 ± 0.286
3.631PheVal: 3.631 ± 0.349
0.437PheTrp: 0.437 ± 0.1
1.867PheTyr: 1.867 ± 0.171
0.0PheXaa: 0.0 ± 0.0
Gly
5.264GlyAla: 5.264 ± 0.831
0.51GlyCys: 0.51 ± 0.097
5.906GlyAsp: 5.906 ± 0.53
4.798GlyGlu: 4.798 ± 0.297
3.675GlyPhe: 3.675 ± 0.285
7.918GlyGly: 7.918 ± 0.797
1.064GlyHis: 1.064 ± 0.159
5.877GlyIle: 5.877 ± 0.584
3.748GlyLys: 3.748 ± 0.344
5.031GlyLeu: 5.031 ± 0.37
1.808GlyMet: 1.808 ± 0.202
4.462GlyAsn: 4.462 ± 0.377
2.377GlyPro: 2.377 ± 0.497
2.435GlyGln: 2.435 ± 0.212
3.033GlyArg: 3.033 ± 0.222
6.474GlySer: 6.474 ± 0.541
6.139GlyThr: 6.139 ± 0.605
5.993GlyVal: 5.993 ± 0.449
1.064GlyTrp: 1.064 ± 0.148
3.325GlyTyr: 3.325 ± 0.245
0.0GlyXaa: 0.0 ± 0.0
His
0.729HisAla: 0.729 ± 0.098
0.233HisCys: 0.233 ± 0.063
1.006HisAsp: 1.006 ± 0.128
1.079HisGlu: 1.079 ± 0.159
0.86HisPhe: 0.86 ± 0.136
1.094HisGly: 1.094 ± 0.156
0.467HisHis: 0.467 ± 0.094
0.962HisIle: 0.962 ± 0.15
0.89HisLys: 0.89 ± 0.111
1.269HisLeu: 1.269 ± 0.171
0.306HisMet: 0.306 ± 0.079
1.094HisAsn: 1.094 ± 0.164
0.904HisPro: 0.904 ± 0.21
0.525HisGln: 0.525 ± 0.083
0.715HisArg: 0.715 ± 0.116
0.977HisSer: 0.977 ± 0.145
1.225HisThr: 1.225 ± 0.153
0.962HisVal: 0.962 ± 0.116
0.277HisTrp: 0.277 ± 0.073
0.7HisTyr: 0.7 ± 0.137
0.0HisXaa: 0.0 ± 0.0
Ile
3.879IleAla: 3.879 ± 0.278
0.554IleCys: 0.554 ± 0.098
4.768IleAsp: 4.768 ± 0.322
4.243IleGlu: 4.243 ± 0.241
2.581IlePhe: 2.581 ± 0.236
5.206IleGly: 5.206 ± 0.422
1.035IleHis: 1.035 ± 0.133
3.893IleIle: 3.893 ± 0.246
3.733IleLys: 3.733 ± 0.24
4.389IleLeu: 4.389 ± 0.273
1.225IleMet: 1.225 ± 0.182
4.345IleAsn: 4.345 ± 0.288
2.566IlePro: 2.566 ± 0.186
2.406IleGln: 2.406 ± 0.169
2.887IleArg: 2.887 ± 0.203
4.827IleSer: 4.827 ± 0.381
5.6IleThr: 5.6 ± 0.502
3.908IleVal: 3.908 ± 0.236
0.467IleTrp: 0.467 ± 0.102
2.319IleTyr: 2.319 ± 0.269
0.0IleXaa: 0.0 ± 0.0
Lys
3.412LysAla: 3.412 ± 0.332
0.525LysCys: 0.525 ± 0.1
3.31LysAsp: 3.31 ± 0.309
4.316LysGlu: 4.316 ± 0.463
2.494LysPhe: 2.494 ± 0.245
3.471LysGly: 3.471 ± 0.26
1.006LysHis: 1.006 ± 0.137
3.398LysIle: 3.398 ± 0.268
4.36LysLys: 4.36 ± 0.579
4.695LysLeu: 4.695 ± 0.327
1.531LysMet: 1.531 ± 0.168
2.785LysAsn: 2.785 ± 0.205
2.231LysPro: 2.231 ± 0.274
2.027LysGln: 2.027 ± 0.211
2.756LysArg: 2.756 ± 0.276
3.441LysSer: 3.441 ± 0.329
3.091LysThr: 3.091 ± 0.222
4.243LysVal: 4.243 ± 0.261
0.583LysTrp: 0.583 ± 0.119
2.391LysTyr: 2.391 ± 0.252
0.0LysXaa: 0.0 ± 0.0
Leu
4.243LeuAla: 4.243 ± 0.305
0.598LeuCys: 0.598 ± 0.13
5.293LeuAsp: 5.293 ± 0.294
5.104LeuGlu: 5.104 ± 0.32
2.902LeuPhe: 2.902 ± 0.22
4.754LeuGly: 4.754 ± 0.325
1.327LeuHis: 1.327 ± 0.187
3.996LeuIle: 3.996 ± 0.256
4.623LeuLys: 4.623 ± 0.313
5.002LeuLeu: 5.002 ± 0.427
1.954LeuMet: 1.954 ± 0.239
4.462LeuAsn: 4.462 ± 0.286
2.698LeuPro: 2.698 ± 0.214
2.421LeuGln: 2.421 ± 0.166
3.587LeuArg: 3.587 ± 0.216
5.804LeuSer: 5.804 ± 0.322
5.512LeuThr: 5.512 ± 0.391
4.448LeuVal: 4.448 ± 0.258
0.598LeuTrp: 0.598 ± 0.111
3.616LeuTyr: 3.616 ± 0.23
0.0LeuXaa: 0.0 ± 0.0
Met
1.794MetAla: 1.794 ± 0.199
0.335MetCys: 0.335 ± 0.076
1.444MetAsp: 1.444 ± 0.184
1.429MetGlu: 1.429 ± 0.157
0.919MetPhe: 0.919 ± 0.156
1.283MetGly: 1.283 ± 0.173
0.292MetHis: 0.292 ± 0.08
1.371MetIle: 1.371 ± 0.162
1.662MetLys: 1.662 ± 0.214
1.064MetLeu: 1.064 ± 0.183
0.525MetMet: 0.525 ± 0.11
1.444MetAsn: 1.444 ± 0.182
0.787MetPro: 0.787 ± 0.116
0.598MetGln: 0.598 ± 0.107
0.948MetArg: 0.948 ± 0.133
1.794MetSer: 1.794 ± 0.206
1.473MetThr: 1.473 ± 0.145
1.196MetVal: 1.196 ± 0.15
0.262MetTrp: 0.262 ± 0.075
0.831MetTyr: 0.831 ± 0.136
0.0MetXaa: 0.0 ± 0.0
Asn
2.712AsnAla: 2.712 ± 0.248
0.496AsnCys: 0.496 ± 0.099
3.033AsnAsp: 3.033 ± 0.189
3.237AsnGlu: 3.237 ± 0.206
2.727AsnPhe: 2.727 ± 0.178
4.273AsnGly: 4.273 ± 0.268
0.992AsnHis: 0.992 ± 0.156
4.9AsnIle: 4.9 ± 0.307
3.193AsnLys: 3.193 ± 0.22
4.535AsnLeu: 4.535 ± 0.361
0.948AsnMet: 0.948 ± 0.149
3.354AsnAsn: 3.354 ± 0.29
2.975AsnPro: 2.975 ± 0.279
2.129AsnGln: 2.129 ± 0.183
2.202AsnArg: 2.202 ± 0.167
4.054AsnSer: 4.054 ± 0.295
3.85AsnThr: 3.85 ± 0.254
4.579AsnVal: 4.579 ± 0.449
0.656AsnTrp: 0.656 ± 0.099
2.362AsnTyr: 2.362 ± 0.162
0.0AsnXaa: 0.0 ± 0.0
Pro
2.231ProAla: 2.231 ± 0.206
0.262ProCys: 0.262 ± 0.061
3.193ProAsp: 3.193 ± 0.271
2.771ProGlu: 2.771 ± 0.263
1.604ProPhe: 1.604 ± 0.176
3.354ProGly: 3.354 ± 0.352
1.181ProHis: 1.181 ± 0.164
2.231ProIle: 2.231 ± 0.202
2.012ProLys: 2.012 ± 0.297
2.319ProLeu: 2.319 ± 0.236
0.685ProMet: 0.685 ± 0.11
2.45ProAsn: 2.45 ± 0.183
1.823ProPro: 1.823 ± 0.22
1.458ProGln: 1.458 ± 0.184
1.502ProArg: 1.502 ± 0.138
2.771ProSer: 2.771 ± 0.197
3.062ProThr: 3.062 ± 0.209
2.698ProVal: 2.698 ± 0.233
0.496ProTrp: 0.496 ± 0.088
1.531ProTyr: 1.531 ± 0.126
0.0ProXaa: 0.0 ± 0.0
Gln
1.823GlnAla: 1.823 ± 0.179
0.233GlnCys: 0.233 ± 0.054
1.939GlnAsp: 1.939 ± 0.199
1.721GlnGlu: 1.721 ± 0.166
1.692GlnPhe: 1.692 ± 0.16
2.391GlnGly: 2.391 ± 0.224
0.496GlnHis: 0.496 ± 0.105
2.144GlnIle: 2.144 ± 0.179
2.056GlnLys: 2.056 ± 0.233
2.96GlnLeu: 2.96 ± 0.222
1.021GlnMet: 1.021 ± 0.144
1.633GlnAsn: 1.633 ± 0.127
1.137GlnPro: 1.137 ± 0.119
1.342GlnGln: 1.342 ± 0.202
1.546GlnArg: 1.546 ± 0.159
2.625GlnSer: 2.625 ± 0.211
2.216GlnThr: 2.216 ± 0.19
2.464GlnVal: 2.464 ± 0.203
0.554GlnTrp: 0.554 ± 0.113
1.662GlnTyr: 1.662 ± 0.15
0.0GlnXaa: 0.0 ± 0.0
Arg
2.319ArgAla: 2.319 ± 0.227
0.452ArgCys: 0.452 ± 0.079
2.785ArgAsp: 2.785 ± 0.194
2.989ArgGlu: 2.989 ± 0.282
1.852ArgPhe: 1.852 ± 0.166
3.427ArgGly: 3.427 ± 0.204
0.525ArgHis: 0.525 ± 0.108
2.639ArgIle: 2.639 ± 0.199
2.873ArgLys: 2.873 ± 0.278
2.96ArgLeu: 2.96 ± 0.21
1.312ArgMet: 1.312 ± 0.145
2.173ArgAsn: 2.173 ± 0.169
1.75ArgPro: 1.75 ± 0.195
1.254ArgGln: 1.254 ± 0.132
1.837ArgArg: 1.837 ± 0.193
2.669ArgSer: 2.669 ± 0.195
2.231ArgThr: 2.231 ± 0.221
3.179ArgVal: 3.179 ± 0.248
0.583ArgTrp: 0.583 ± 0.115
2.173ArgTyr: 2.173 ± 0.181
0.0ArgXaa: 0.0 ± 0.0
Ser
4.302SerAla: 4.302 ± 0.391
0.656SerCys: 0.656 ± 0.134
4.229SerAsp: 4.229 ± 0.268
4.652SerGlu: 4.652 ± 0.355
3.427SerPhe: 3.427 ± 0.211
7.379SerGly: 7.379 ± 0.637
1.444SerHis: 1.444 ± 0.177
5.235SerIle: 5.235 ± 0.351
3.281SerLys: 3.281 ± 0.297
5.016SerLeu: 5.016 ± 0.343
1.123SerMet: 1.123 ± 0.135
3.791SerAsn: 3.791 ± 0.274
2.669SerPro: 2.669 ± 0.23
2.464SerGln: 2.464 ± 0.209
2.61SerArg: 2.61 ± 0.21
6.008SerSer: 6.008 ± 0.576
5.789SerThr: 5.789 ± 0.492
5.162SerVal: 5.162 ± 0.332
0.773SerTrp: 0.773 ± 0.127
3.004SerTyr: 3.004 ± 0.223
0.0SerXaa: 0.0 ± 0.0
Thr
4.054ThrAla: 4.054 ± 0.333
0.612ThrCys: 0.612 ± 0.087
4.9ThrAsp: 4.9 ± 0.345
4.389ThrGlu: 4.389 ± 0.3
3.543ThrPhe: 3.543 ± 0.385
7.495ThrGly: 7.495 ± 1.125
1.094ThrHis: 1.094 ± 0.139
5.118ThrIle: 5.118 ± 0.477
3.383ThrLys: 3.383 ± 0.263
5.629ThrLeu: 5.629 ± 0.379
0.977ThrMet: 0.977 ± 0.132
4.491ThrAsn: 4.491 ± 0.422
3.543ThrPro: 3.543 ± 0.245
2.698ThrGln: 2.698 ± 0.23
2.406ThrArg: 2.406 ± 0.19
4.827ThrSer: 4.827 ± 0.366
6.256ThrThr: 6.256 ± 0.659
4.841ThrVal: 4.841 ± 0.47
0.612ThrTrp: 0.612 ± 0.094
2.698ThrTyr: 2.698 ± 0.226
0.0ThrXaa: 0.0 ± 0.0
Val
4.564ValAla: 4.564 ± 0.288
0.671ValCys: 0.671 ± 0.115
4.885ValAsp: 4.885 ± 0.366
4.448ValGlu: 4.448 ± 0.278
3.091ValPhe: 3.091 ± 0.22
6.693ValGly: 6.693 ± 0.912
0.715ValHis: 0.715 ± 0.1
4.156ValIle: 4.156 ± 0.314
3.631ValLys: 3.631 ± 0.278
4.681ValLeu: 4.681 ± 0.265
1.342ValMet: 1.342 ± 0.154
3.514ValAsn: 3.514 ± 0.242
2.508ValPro: 2.508 ± 0.184
2.187ValGln: 2.187 ± 0.177
3.048ValArg: 3.048 ± 0.263
6.329ValSer: 6.329 ± 0.461
6.27ValThr: 6.27 ± 0.658
5.497ValVal: 5.497 ± 0.352
0.685ValTrp: 0.685 ± 0.122
2.581ValTyr: 2.581 ± 0.199
0.0ValXaa: 0.0 ± 0.0
Trp
0.554TrpAla: 0.554 ± 0.104
0.102TrpCys: 0.102 ± 0.044
0.627TrpAsp: 0.627 ± 0.129
0.831TrpGlu: 0.831 ± 0.13
0.612TrpPhe: 0.612 ± 0.115
1.05TrpGly: 1.05 ± 0.148
0.306TrpHis: 0.306 ± 0.069
0.671TrpIle: 0.671 ± 0.117
0.802TrpLys: 0.802 ± 0.127
0.744TrpLeu: 0.744 ± 0.142
0.306TrpMet: 0.306 ± 0.076
0.787TrpAsn: 0.787 ± 0.12
0.146TrpPro: 0.146 ± 0.049
0.365TrpGln: 0.365 ± 0.075
0.525TrpArg: 0.525 ± 0.094
0.54TrpSer: 0.54 ± 0.113
0.7TrpThr: 0.7 ± 0.11
0.86TrpVal: 0.86 ± 0.15
0.131TrpTrp: 0.131 ± 0.039
0.452TrpTyr: 0.452 ± 0.097
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.173TyrAla: 2.173 ± 0.172
0.408TyrCys: 0.408 ± 0.085
3.543TyrAsp: 3.543 ± 0.313
2.596TyrGlu: 2.596 ± 0.236
1.75TyrPhe: 1.75 ± 0.2
3.019TyrGly: 3.019 ± 0.226
0.598TyrHis: 0.598 ± 0.102
2.756TyrIle: 2.756 ± 0.273
2.056TyrLys: 2.056 ± 0.206
3.252TyrLeu: 3.252 ± 0.238
0.729TyrMet: 0.729 ± 0.124
3.019TyrAsn: 3.019 ± 0.192
1.706TyrPro: 1.706 ± 0.17
1.444TyrGln: 1.444 ± 0.181
1.939TyrArg: 1.939 ± 0.171
2.931TyrSer: 2.931 ± 0.234
2.464TyrThr: 2.464 ± 0.166
3.412TyrVal: 3.412 ± 0.229
0.437TyrTrp: 0.437 ± 0.093
1.896TyrTyr: 1.896 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 286 proteins (68578 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski