Amino acid dipepetide frequency for Staphylococcus phage SN13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.45AlaAla: 2.45 ± 0.407
0.316AlaCys: 0.316 ± 0.153
3.794AlaAsp: 3.794 ± 0.623
5.532AlaGlu: 5.532 ± 0.678
2.45AlaPhe: 2.45 ± 0.457
2.766AlaGly: 2.766 ± 0.59
0.948AlaHis: 0.948 ± 0.221
6.006AlaIle: 6.006 ± 1.15
5.69AlaLys: 5.69 ± 0.719
5.69AlaLeu: 5.69 ± 0.649
1.265AlaMet: 1.265 ± 0.292
4.505AlaAsn: 4.505 ± 0.652
0.948AlaPro: 0.948 ± 0.199
2.687AlaGln: 2.687 ± 0.459
2.529AlaArg: 2.529 ± 0.471
2.529AlaSer: 2.529 ± 0.543
4.426AlaThr: 4.426 ± 0.653
3.556AlaVal: 3.556 ± 0.572
0.395AlaTrp: 0.395 ± 0.207
2.134AlaTyr: 2.134 ± 0.462
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.079CysAsp: 0.079 ± 0.081
0.158CysGlu: 0.158 ± 0.127
0.0CysPhe: 0.0 ± 0.0
0.237CysGly: 0.237 ± 0.139
0.158CysHis: 0.158 ± 0.11
0.316CysIle: 0.316 ± 0.17
0.395CysLys: 0.395 ± 0.164
0.237CysLeu: 0.237 ± 0.192
0.316CysMet: 0.316 ± 0.187
0.474CysAsn: 0.474 ± 0.202
0.0CysPro: 0.0 ± 0.0
0.316CysGln: 0.316 ± 0.168
0.395CysArg: 0.395 ± 0.19
0.237CysSer: 0.237 ± 0.119
0.316CysThr: 0.316 ± 0.155
0.079CysVal: 0.079 ± 0.091
0.079CysTrp: 0.079 ± 0.077
0.158CysTyr: 0.158 ± 0.109
0.0CysXaa: 0.0 ± 0.0
Asp
3.477AspAla: 3.477 ± 0.577
0.079AspCys: 0.079 ± 0.077
5.532AspAsp: 5.532 ± 0.891
6.718AspGlu: 6.718 ± 0.863
3.319AspPhe: 3.319 ± 0.546
3.636AspGly: 3.636 ± 0.537
0.632AspHis: 0.632 ± 0.225
5.532AspIle: 5.532 ± 0.639
5.532AspLys: 5.532 ± 0.741
5.216AspLeu: 5.216 ± 0.753
1.423AspMet: 1.423 ± 0.353
4.031AspAsn: 4.031 ± 0.652
1.185AspPro: 1.185 ± 0.258
0.869AspGln: 0.869 ± 0.242
2.292AspArg: 2.292 ± 0.389
4.979AspSer: 4.979 ± 0.649
3.161AspThr: 3.161 ± 0.443
4.268AspVal: 4.268 ± 0.481
0.474AspTrp: 0.474 ± 0.202
3.319AspTyr: 3.319 ± 0.483
0.0AspXaa: 0.0 ± 0.0
Glu
5.137GluAla: 5.137 ± 0.583
0.632GluCys: 0.632 ± 0.244
3.477GluAsp: 3.477 ± 0.519
7.508GluGlu: 7.508 ± 1.128
4.742GluPhe: 4.742 ± 0.54
2.766GluGly: 2.766 ± 0.488
0.711GluHis: 0.711 ± 0.233
5.216GluIle: 5.216 ± 0.759
5.69GluLys: 5.69 ± 0.82
6.402GluLeu: 6.402 ± 0.698
2.608GluMet: 2.608 ± 0.408
4.584GluAsn: 4.584 ± 0.749
1.502GluPro: 1.502 ± 0.443
3.319GluGln: 3.319 ± 0.48
4.663GluArg: 4.663 ± 0.802
4.189GluSer: 4.189 ± 0.619
2.845GluThr: 2.845 ± 0.575
5.69GluVal: 5.69 ± 0.646
0.711GluTrp: 0.711 ± 0.233
3.24GluTyr: 3.24 ± 0.484
0.0GluXaa: 0.0 ± 0.0
Phe
2.45PheAla: 2.45 ± 0.498
0.158PheCys: 0.158 ± 0.118
2.529PheAsp: 2.529 ± 0.534
3.952PheGlu: 3.952 ± 0.585
1.897PhePhe: 1.897 ± 0.362
3.082PheGly: 3.082 ± 0.494
0.316PheHis: 0.316 ± 0.165
3.319PheIle: 3.319 ± 0.513
4.347PheLys: 4.347 ± 0.469
2.608PheLeu: 2.608 ± 0.549
1.423PheMet: 1.423 ± 0.327
2.924PheAsn: 2.924 ± 0.531
0.869PhePro: 0.869 ± 0.233
0.869PheGln: 0.869 ± 0.293
1.027PheArg: 1.027 ± 0.28
2.529PheSer: 2.529 ± 0.55
2.608PheThr: 2.608 ± 0.483
4.031PheVal: 4.031 ± 0.634
0.474PheTrp: 0.474 ± 0.202
1.66PheTyr: 1.66 ± 0.335
0.0PheXaa: 0.0 ± 0.0
Gly
2.766GlyAla: 2.766 ± 0.748
0.316GlyCys: 0.316 ± 0.146
2.529GlyAsp: 2.529 ± 0.478
3.319GlyGlu: 3.319 ± 0.54
2.845GlyPhe: 2.845 ± 0.57
1.739GlyGly: 1.739 ± 0.38
0.869GlyHis: 0.869 ± 0.241
3.715GlyIle: 3.715 ± 0.521
4.742GlyLys: 4.742 ± 0.601
4.584GlyLeu: 4.584 ± 0.76
0.948GlyMet: 0.948 ± 0.271
2.608GlyAsn: 2.608 ± 0.492
0.474GlyPro: 0.474 ± 0.244
1.976GlyGln: 1.976 ± 0.466
2.371GlyArg: 2.371 ± 0.437
2.292GlySer: 2.292 ± 0.441
3.477GlyThr: 3.477 ± 0.505
4.742GlyVal: 4.742 ± 0.701
0.474GlyTrp: 0.474 ± 0.202
3.24GlyTyr: 3.24 ± 0.588
0.0GlyXaa: 0.0 ± 0.0
His
0.553HisAla: 0.553 ± 0.238
0.237HisCys: 0.237 ± 0.114
1.027HisAsp: 1.027 ± 0.278
1.027HisGlu: 1.027 ± 0.382
1.027HisPhe: 1.027 ± 0.332
1.185HisGly: 1.185 ± 0.357
0.316HisHis: 0.316 ± 0.16
1.265HisIle: 1.265 ± 0.41
1.344HisLys: 1.344 ± 0.333
1.739HisLeu: 1.739 ± 0.334
0.316HisMet: 0.316 ± 0.158
0.948HisAsn: 0.948 ± 0.305
0.395HisPro: 0.395 ± 0.185
1.185HisGln: 1.185 ± 0.312
0.158HisArg: 0.158 ± 0.16
0.711HisSer: 0.711 ± 0.251
0.948HisThr: 0.948 ± 0.237
0.948HisVal: 0.948 ± 0.279
0.079HisTrp: 0.079 ± 0.084
1.265HisTyr: 1.265 ± 0.307
0.0HisXaa: 0.0 ± 0.0
Ile
4.821IleAla: 4.821 ± 0.753
0.316IleCys: 0.316 ± 0.166
6.718IleAsp: 6.718 ± 0.664
5.295IleGlu: 5.295 ± 0.793
2.766IlePhe: 2.766 ± 0.485
3.794IleGly: 3.794 ± 0.96
1.027IleHis: 1.027 ± 0.315
4.031IleIle: 4.031 ± 0.497
8.694IleLys: 8.694 ± 0.889
4.663IleLeu: 4.663 ± 0.64
1.185IleMet: 1.185 ± 0.354
5.374IleAsn: 5.374 ± 0.606
2.687IlePro: 2.687 ± 0.374
2.213IleGln: 2.213 ± 0.602
2.608IleArg: 2.608 ± 0.429
4.584IleSer: 4.584 ± 0.602
4.268IleThr: 4.268 ± 0.581
5.216IleVal: 5.216 ± 0.5
1.027IleTrp: 1.027 ± 0.311
3.398IleTyr: 3.398 ± 0.622
0.0IleXaa: 0.0 ± 0.0
Lys
6.402LysAla: 6.402 ± 0.783
0.158LysCys: 0.158 ± 0.112
6.481LysAsp: 6.481 ± 0.727
6.955LysGlu: 6.955 ± 1.097
3.24LysPhe: 3.24 ± 0.474
4.742LysGly: 4.742 ± 0.669
1.818LysHis: 1.818 ± 0.41
7.113LysIle: 7.113 ± 0.678
8.061LysLys: 8.061 ± 0.944
7.429LysLeu: 7.429 ± 0.75
2.766LysMet: 2.766 ± 0.436
4.426LysAsn: 4.426 ± 0.659
3.003LysPro: 3.003 ± 0.943
4.979LysGln: 4.979 ± 0.569
4.11LysArg: 4.11 ± 0.686
5.532LysSer: 5.532 ± 0.797
5.532LysThr: 5.532 ± 0.729
5.69LysVal: 5.69 ± 0.572
0.711LysTrp: 0.711 ± 0.227
4.426LysTyr: 4.426 ± 0.788
0.0LysXaa: 0.0 ± 0.0
Leu
5.295LeuAla: 5.295 ± 0.598
0.316LeuCys: 0.316 ± 0.223
6.244LeuAsp: 6.244 ± 0.628
6.006LeuGlu: 6.006 ± 0.715
2.924LeuPhe: 2.924 ± 0.517
3.398LeuGly: 3.398 ± 0.672
1.344LeuHis: 1.344 ± 0.284
5.69LeuIle: 5.69 ± 0.755
7.192LeuLys: 7.192 ± 0.749
5.69LeuLeu: 5.69 ± 0.678
2.055LeuMet: 2.055 ± 0.419
6.006LeuAsn: 6.006 ± 0.765
2.213LeuPro: 2.213 ± 0.472
3.161LeuGln: 3.161 ± 0.4
3.24LeuArg: 3.24 ± 0.657
5.611LeuSer: 5.611 ± 0.925
5.848LeuThr: 5.848 ± 0.696
3.952LeuVal: 3.952 ± 0.692
1.027LeuTrp: 1.027 ± 0.387
2.134LeuTyr: 2.134 ± 0.403
0.0LeuXaa: 0.0 ± 0.0
Met
1.976MetAla: 1.976 ± 0.348
0.079MetCys: 0.079 ± 0.087
1.818MetAsp: 1.818 ± 0.397
1.818MetGlu: 1.818 ± 0.423
1.027MetPhe: 1.027 ± 0.295
0.79MetGly: 0.79 ± 0.225
0.395MetHis: 0.395 ± 0.157
1.739MetIle: 1.739 ± 0.433
2.213MetLys: 2.213 ± 0.394
1.739MetLeu: 1.739 ± 0.363
0.869MetMet: 0.869 ± 0.21
1.027MetAsn: 1.027 ± 0.299
0.395MetPro: 0.395 ± 0.165
0.711MetGln: 0.711 ± 0.251
1.344MetArg: 1.344 ± 0.352
1.897MetSer: 1.897 ± 0.426
1.66MetThr: 1.66 ± 0.399
0.79MetVal: 0.79 ± 0.22
0.237MetTrp: 0.237 ± 0.112
1.106MetTyr: 1.106 ± 0.297
0.0MetXaa: 0.0 ± 0.0
Asn
4.821AsnAla: 4.821 ± 0.684
0.316AsnCys: 0.316 ± 0.206
4.189AsnAsp: 4.189 ± 0.739
4.742AsnGlu: 4.742 ± 0.634
2.371AsnPhe: 2.371 ± 0.304
5.137AsnGly: 5.137 ± 0.559
0.869AsnHis: 0.869 ± 0.233
3.24AsnIle: 3.24 ± 0.421
6.718AsnLys: 6.718 ± 0.692
5.295AsnLeu: 5.295 ± 0.755
1.502AsnMet: 1.502 ± 0.359
4.9AsnAsn: 4.9 ± 0.839
2.45AsnPro: 2.45 ± 0.427
1.739AsnGln: 1.739 ± 0.368
2.213AsnArg: 2.213 ± 0.439
4.11AsnSer: 4.11 ± 0.526
3.952AsnThr: 3.952 ± 0.527
4.347AsnVal: 4.347 ± 0.647
1.106AsnTrp: 1.106 ± 0.335
2.766AsnTyr: 2.766 ± 0.584
0.0AsnXaa: 0.0 ± 0.0
Pro
1.344ProAla: 1.344 ± 0.382
0.079ProCys: 0.079 ± 0.078
1.185ProAsp: 1.185 ± 0.379
1.344ProGlu: 1.344 ± 0.428
0.948ProPhe: 0.948 ± 0.322
0.632ProGly: 0.632 ± 0.261
0.632ProHis: 0.632 ± 0.236
2.134ProIle: 2.134 ± 0.459
3.477ProLys: 3.477 ± 0.874
1.185ProLeu: 1.185 ± 0.376
0.474ProMet: 0.474 ± 0.194
2.687ProAsn: 2.687 ± 0.494
0.869ProPro: 0.869 ± 0.404
1.185ProGln: 1.185 ± 0.503
0.869ProArg: 0.869 ± 0.247
2.292ProSer: 2.292 ± 0.466
1.502ProThr: 1.502 ± 0.285
1.818ProVal: 1.818 ± 0.399
0.0ProTrp: 0.0 ± 0.0
1.185ProTyr: 1.185 ± 0.317
0.0ProXaa: 0.0 ± 0.0
Gln
2.529GlnAla: 2.529 ± 0.433
0.316GlnCys: 0.316 ± 0.175
1.739GlnAsp: 1.739 ± 0.303
1.897GlnGlu: 1.897 ± 0.531
1.66GlnPhe: 1.66 ± 0.352
1.344GlnGly: 1.344 ± 0.302
0.79GlnHis: 0.79 ± 0.262
2.371GlnIle: 2.371 ± 0.462
4.031GlnLys: 4.031 ± 0.561
3.319GlnLeu: 3.319 ± 0.596
0.395GlnMet: 0.395 ± 0.159
3.873GlnAsn: 3.873 ± 0.703
0.79GlnPro: 0.79 ± 0.422
2.529GlnGln: 2.529 ± 0.798
1.818GlnArg: 1.818 ± 0.33
2.055GlnSer: 2.055 ± 0.406
3.161GlnThr: 3.161 ± 0.625
2.213GlnVal: 2.213 ± 0.372
0.158GlnTrp: 0.158 ± 0.11
1.66GlnTyr: 1.66 ± 0.415
0.0GlnXaa: 0.0 ± 0.0
Arg
2.134ArgAla: 2.134 ± 0.433
0.158ArgCys: 0.158 ± 0.131
2.292ArgAsp: 2.292 ± 0.47
2.608ArgGlu: 2.608 ± 0.465
2.055ArgPhe: 2.055 ± 0.393
1.739ArgGly: 1.739 ± 0.414
1.265ArgHis: 1.265 ± 0.319
3.477ArgIle: 3.477 ± 0.482
3.24ArgLys: 3.24 ± 0.511
4.347ArgLeu: 4.347 ± 0.582
1.265ArgMet: 1.265 ± 0.343
2.213ArgAsn: 2.213 ± 0.423
1.106ArgPro: 1.106 ± 0.373
1.897ArgGln: 1.897 ± 0.376
1.66ArgArg: 1.66 ± 0.337
2.055ArgSer: 2.055 ± 0.439
1.976ArgThr: 1.976 ± 0.441
2.45ArgVal: 2.45 ± 0.383
0.474ArgTrp: 0.474 ± 0.192
2.766ArgTyr: 2.766 ± 0.398
0.0ArgXaa: 0.0 ± 0.0
Ser
3.556SerAla: 3.556 ± 0.643
0.158SerCys: 0.158 ± 0.12
3.398SerAsp: 3.398 ± 0.578
4.426SerGlu: 4.426 ± 0.473
2.213SerPhe: 2.213 ± 0.598
3.794SerGly: 3.794 ± 0.51
1.265SerHis: 1.265 ± 0.337
6.086SerIle: 6.086 ± 0.965
5.69SerLys: 5.69 ± 0.723
5.848SerLeu: 5.848 ± 0.776
1.027SerMet: 1.027 ± 0.288
4.11SerAsn: 4.11 ± 0.536
1.265SerPro: 1.265 ± 0.282
1.739SerGln: 1.739 ± 0.383
2.924SerArg: 2.924 ± 0.447
3.319SerSer: 3.319 ± 0.614
3.715SerThr: 3.715 ± 0.554
3.715SerVal: 3.715 ± 0.502
0.395SerTrp: 0.395 ± 0.203
2.213SerTyr: 2.213 ± 0.478
0.0SerXaa: 0.0 ± 0.0
Thr
3.398ThrAla: 3.398 ± 0.491
0.079ThrCys: 0.079 ± 0.092
3.715ThrAsp: 3.715 ± 0.577
3.873ThrGlu: 3.873 ± 0.541
2.45ThrPhe: 2.45 ± 0.472
3.319ThrGly: 3.319 ± 0.65
1.265ThrHis: 1.265 ± 0.26
4.821ThrIle: 4.821 ± 0.746
5.769ThrLys: 5.769 ± 0.639
5.058ThrLeu: 5.058 ± 0.64
1.185ThrMet: 1.185 ± 0.305
3.319ThrAsn: 3.319 ± 0.568
2.213ThrPro: 2.213 ± 0.36
2.213ThrGln: 2.213 ± 0.472
2.687ThrArg: 2.687 ± 0.491
3.24ThrSer: 3.24 ± 0.596
3.952ThrThr: 3.952 ± 0.707
4.268ThrVal: 4.268 ± 0.894
0.711ThrTrp: 0.711 ± 0.323
2.608ThrTyr: 2.608 ± 0.401
0.0ThrXaa: 0.0 ± 0.0
Val
4.189ValAla: 4.189 ± 0.639
0.158ValCys: 0.158 ± 0.105
5.137ValAsp: 5.137 ± 0.491
5.453ValGlu: 5.453 ± 0.719
2.371ValPhe: 2.371 ± 0.49
3.24ValGly: 3.24 ± 0.584
0.79ValHis: 0.79 ± 0.2
5.137ValIle: 5.137 ± 0.495
4.9ValLys: 4.9 ± 0.604
4.821ValLeu: 4.821 ± 0.62
1.344ValMet: 1.344 ± 0.424
4.9ValAsn: 4.9 ± 0.592
2.213ValPro: 2.213 ± 0.399
2.134ValGln: 2.134 ± 0.392
1.818ValArg: 1.818 ± 0.337
5.453ValSer: 5.453 ± 0.608
4.11ValThr: 4.11 ± 0.754
4.347ValVal: 4.347 ± 0.676
0.79ValTrp: 0.79 ± 0.211
2.292ValTyr: 2.292 ± 0.469
0.0ValXaa: 0.0 ± 0.0
Trp
0.948TrpAla: 0.948 ± 0.286
0.079TrpCys: 0.079 ± 0.074
0.474TrpAsp: 0.474 ± 0.168
0.316TrpGlu: 0.316 ± 0.152
0.316TrpPhe: 0.316 ± 0.181
0.316TrpGly: 0.316 ± 0.191
0.237TrpHis: 0.237 ± 0.145
0.632TrpIle: 0.632 ± 0.233
0.632TrpLys: 0.632 ± 0.26
0.632TrpLeu: 0.632 ± 0.198
0.079TrpMet: 0.079 ± 0.077
1.106TrpAsn: 1.106 ± 0.571
0.158TrpPro: 0.158 ± 0.11
0.395TrpGln: 0.395 ± 0.244
0.395TrpArg: 0.395 ± 0.172
1.185TrpSer: 1.185 ± 0.315
0.711TrpThr: 0.711 ± 0.228
0.948TrpVal: 0.948 ± 0.235
0.079TrpTrp: 0.079 ± 0.085
0.553TrpTyr: 0.553 ± 0.212
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.371TyrAla: 2.371 ± 0.407
0.0TyrCys: 0.0 ± 0.0
3.398TyrAsp: 3.398 ± 0.626
2.687TyrGlu: 2.687 ± 0.479
2.45TyrPhe: 2.45 ± 0.575
2.371TyrGly: 2.371 ± 0.465
1.027TyrHis: 1.027 ± 0.322
2.845TyrIle: 2.845 ± 0.609
5.216TyrLys: 5.216 ± 0.764
2.845TyrLeu: 2.845 ± 0.575
1.106TyrMet: 1.106 ± 0.334
2.766TyrAsn: 2.766 ± 0.447
1.106TyrPro: 1.106 ± 0.278
2.529TyrGln: 2.529 ± 0.622
2.055TyrArg: 2.055 ± 0.391
2.055TyrSer: 2.055 ± 0.503
1.976TyrThr: 1.976 ± 0.377
2.529TyrVal: 2.529 ± 0.464
0.79TyrTrp: 0.79 ± 0.293
1.739TyrTyr: 1.739 ± 0.456
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (12654 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski