Ganoderma sinense ZZ0214-1

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Polyporaceae; Ganoderma; Ganoderma sinense

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15394 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G8S310|A0A2G8S310_9APHY Transcription factor OS=Ganoderma sinense ZZ0214-1 OX=1077348 GN=GSI_09696 PE=4 SV=1
MM1 pKa = 7.46FLPLTLLAALPLFPVWAAPMCSLKK25 pKa = 10.53PPKK28 pKa = 9.89SASATSSAPAPTGTTVGATGSNSNGAQVVATTWYY62 pKa = 10.16AGWHH66 pKa = 5.46GTDD69 pKa = 4.58FPPEE73 pKa = 3.64NVTWSKK79 pKa = 11.41YY80 pKa = 9.46SAATYY85 pKa = 10.39AFAVTTPDD93 pKa = 3.2VNTVSLADD101 pKa = 3.66SDD103 pKa = 5.11EE104 pKa = 4.12EE105 pKa = 4.08LLPRR109 pKa = 11.84FVDD112 pKa = 3.58QAHH115 pKa = 5.76QNDD118 pKa = 3.85VRR120 pKa = 11.84AMLTIGGWTGSQYY133 pKa = 10.5FSSAVATEE141 pKa = 4.03ANRR144 pKa = 11.84TAFVKK149 pKa = 10.14TMTNLISKK157 pKa = 10.05YY158 pKa = 10.5NLDD161 pKa = 3.82GLDD164 pKa = 4.4FDD166 pKa = 4.25WEE168 pKa = 4.49YY169 pKa = 10.89PNKK172 pKa = 10.11QGAGCNIISDD182 pKa = 3.79NDD184 pKa = 3.74SQNFLSFLQSLRR196 pKa = 11.84SAVGPDD202 pKa = 3.31VTLTAAAGITPFAGTDD218 pKa = 3.65GTPMTDD224 pKa = 2.45VSGFAKK230 pKa = 10.52VLDD233 pKa = 3.68WVAIMNYY240 pKa = 9.44DD241 pKa = 3.06VWGSWSSSVGPNAPLNDD258 pKa = 3.78TCASAADD265 pKa = 3.85QEE267 pKa = 4.83GSAVSAVKK275 pKa = 10.19AWTTAGFPANQLLLGVAAYY294 pKa = 9.26GHH296 pKa = 6.14SFSVPTTDD304 pKa = 4.86ALASSSSSNTDD315 pKa = 2.68SSQSALTAYY324 pKa = 9.45PAFDD328 pKa = 4.78KK329 pKa = 11.2SNQPMGDD336 pKa = 2.73SWDD339 pKa = 3.76AVSAEE344 pKa = 3.81SDD346 pKa = 3.26MCGVVTAAGPSGIFDD361 pKa = 3.43FWGLVEE367 pKa = 5.63GGFLTTNGTAASGIDD382 pKa = 3.6YY383 pKa = 10.8RR384 pKa = 11.84FDD386 pKa = 3.5ACSQTPYY393 pKa = 10.45VYY395 pKa = 10.71NPSTQVMVSYY405 pKa = 11.36DD406 pKa = 3.57DD407 pKa = 3.73TRR409 pKa = 11.84SFAAKK414 pKa = 10.07GKK416 pKa = 9.98FINDD420 pKa = 3.08QGLLGFAMWEE430 pKa = 3.78AAGDD434 pKa = 4.03YY435 pKa = 10.91KK436 pKa = 11.72DD437 pKa = 4.18MLLDD441 pKa = 5.26AISDD445 pKa = 4.07AIGIVEE451 pKa = 4.12VSCC454 pKa = 5.0

Molecular weight: 47.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G8SQV3|A0A2G8SQV3_9APHY Uncharacterized protein OS=Ganoderma sinense ZZ0214-1 OX=1077348 GN=GSI_01811 PE=4 SV=1
MM1 pKa = 7.41PRR3 pKa = 11.84IPPSLARR10 pKa = 11.84LLSRR14 pKa = 11.84PPSLPAAVPRR24 pKa = 11.84PATSVSFASSHH35 pKa = 4.86TPFRR39 pKa = 11.84PILPSATPTSSLLRR53 pKa = 11.84LLPTLMTRR61 pKa = 11.84FNSVPSTLSPMFTPLLQVRR80 pKa = 11.84HH81 pKa = 4.5NTKK84 pKa = 9.7GQEE87 pKa = 4.08YY88 pKa = 9.97QPSQRR93 pKa = 11.84KK94 pKa = 8.85RR95 pKa = 11.84KK96 pKa = 9.01RR97 pKa = 11.84KK98 pKa = 9.64HH99 pKa = 5.8GFLTRR104 pKa = 11.84KK105 pKa = 9.7RR106 pKa = 11.84SVNGRR111 pKa = 11.84KK112 pKa = 8.41MLARR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84AKK120 pKa = 10.13GRR122 pKa = 11.84LFLSHH127 pKa = 7.07

Molecular weight: 14.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15394

0

15394

6730433

51

5028

437.2

48.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.393 ± 0.019

1.264 ± 0.007

5.752 ± 0.013

5.788 ± 0.018

3.678 ± 0.011

6.681 ± 0.017

2.637 ± 0.009

4.265 ± 0.013

3.994 ± 0.017

9.251 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.012 ± 0.007

3.02 ± 0.01

7.061 ± 0.028

3.52 ± 0.012

6.601 ± 0.018

8.396 ± 0.025

5.977 ± 0.013

6.639 ± 0.014

1.487 ± 0.007

2.584 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski