Nocardioides sp. BN130099

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B1LKR9|A0A5B1LKR9_9ACTN Ferredoxin OS=Nocardioides sp. BN130099 OX=2607660 GN=F0U44_07175 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.78KK3 pKa = 9.41WIVAIIVVWSLVIVSGIAVAVVLVTGGDD31 pKa = 3.91DD32 pKa = 5.0DD33 pKa = 5.95NDD35 pKa = 3.82AQRR38 pKa = 11.84DD39 pKa = 3.89PSPSPTTPGEE49 pKa = 4.28SSTSPTTPAGPVPAGLEE66 pKa = 4.09SFYY69 pKa = 10.74SQQVAWEE76 pKa = 3.9QCGSDD81 pKa = 2.92KK82 pKa = 11.2CGTLTVPLDD91 pKa = 3.56YY92 pKa = 11.31AEE94 pKa = 4.49PTGEE98 pKa = 4.12TIEE101 pKa = 4.7IALEE105 pKa = 3.75MAPATGEE112 pKa = 4.23RR113 pKa = 11.84IGSLVVNPGGPGAPGTDD130 pKa = 3.15TAKK133 pKa = 10.96DD134 pKa = 3.07ADD136 pKa = 4.32YY137 pKa = 11.45YY138 pKa = 10.49FAPEE142 pKa = 4.18LRR144 pKa = 11.84VAYY147 pKa = 9.71DD148 pKa = 3.06IVGFDD153 pKa = 3.66PRR155 pKa = 11.84GTGDD159 pKa = 4.1SSPIDD164 pKa = 4.37CLPDD168 pKa = 3.92DD169 pKa = 4.77QLDD172 pKa = 3.98EE173 pKa = 4.35YY174 pKa = 11.36LAGDD178 pKa = 4.56PDD180 pKa = 5.39PDD182 pKa = 3.77TKK184 pKa = 11.56AEE186 pKa = 4.13VADD189 pKa = 5.17FNADD193 pKa = 3.47SADD196 pKa = 3.72YY197 pKa = 10.09WSGCAEE203 pKa = 3.93RR204 pKa = 11.84SGDD207 pKa = 3.66LAAHH211 pKa = 6.54VSTIEE216 pKa = 3.71AARR219 pKa = 11.84DD220 pKa = 3.44MDD222 pKa = 4.18VLRR225 pKa = 11.84AALGEE230 pKa = 4.22SQLAYY235 pKa = 10.88LGFSYY240 pKa = 8.75GTRR243 pKa = 11.84LGATYY248 pKa = 10.98ASLFPANVGRR258 pKa = 11.84LVLDD262 pKa = 4.04GAVDD266 pKa = 3.91PSLPTLEE273 pKa = 5.22GSLSQAKK280 pKa = 10.02GFEE283 pKa = 4.19TALRR287 pKa = 11.84SYY289 pKa = 10.49LQNCVDD295 pKa = 5.76LGDD298 pKa = 4.63CFLGDD303 pKa = 4.29SVDD306 pKa = 4.09AGLKK310 pKa = 7.46TVKK313 pKa = 10.46DD314 pKa = 3.82LLADD318 pKa = 3.78IDD320 pKa = 4.11AQPIPTDD327 pKa = 3.9DD328 pKa = 4.25PDD330 pKa = 4.85RR331 pKa = 11.84DD332 pKa = 3.9LTVGRR337 pKa = 11.84AFYY340 pKa = 10.8GVVAPLYY347 pKa = 11.18SKK349 pKa = 11.1DD350 pKa = 2.62NWTYY354 pKa = 11.3LDD356 pKa = 3.47QGLEE360 pKa = 3.9QALDD364 pKa = 3.85GDD366 pKa = 4.35GSTLLFLSDD375 pKa = 5.06FYY377 pKa = 11.0TSRR380 pKa = 11.84NEE382 pKa = 4.09DD383 pKa = 2.54GTYY386 pKa = 10.87ADD388 pKa = 4.47NSVEE392 pKa = 4.3AIGVINCLDD401 pKa = 4.48DD402 pKa = 3.77PWSITSAQVKK412 pKa = 8.13SHH414 pKa = 5.5YY415 pKa = 10.53KK416 pKa = 9.75EE417 pKa = 4.09FEE419 pKa = 3.97KK420 pKa = 10.87ASPTFGDD427 pKa = 3.43VFAWGLVSCNGDD439 pKa = 3.21PFTSTEE445 pKa = 3.75PDD447 pKa = 3.05IPITGAGAAPIVVIGTTRR465 pKa = 11.84DD466 pKa = 3.1PATPYY471 pKa = 11.1AEE473 pKa = 4.08AVAMADD479 pKa = 3.43QLEE482 pKa = 4.46SGVLLSRR489 pKa = 11.84DD490 pKa = 3.62GDD492 pKa = 3.57GHH494 pKa = 4.95TAYY497 pKa = 10.86NKK499 pKa = 10.58GNACIDD505 pKa = 4.22DD506 pKa = 4.07AVHH509 pKa = 7.22AYY511 pKa = 10.57LIDD514 pKa = 3.69GTVPADD520 pKa = 3.5GTEE523 pKa = 4.1CC524 pKa = 4.36

Molecular weight: 55.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B1LN51|A0A5B1LN51_9ACTN Uncharacterized protein OS=Nocardioides sp. BN130099 OX=2607660 GN=F0U44_03725 PE=4 SV=1
MM1 pKa = 7.37TATPTPPDD9 pKa = 3.47RR10 pKa = 11.84RR11 pKa = 11.84SSRR14 pKa = 11.84PVRR17 pKa = 11.84TSRR20 pKa = 11.84RR21 pKa = 11.84LPLLRR26 pKa = 11.84PRR28 pKa = 11.84RR29 pKa = 11.84PRR31 pKa = 11.84RR32 pKa = 11.84PPRR35 pKa = 11.84RR36 pKa = 11.84AAPPRR41 pKa = 11.84SRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84WRR47 pKa = 11.84RR48 pKa = 11.84CVRR51 pKa = 11.84PTPSWSRR58 pKa = 11.84PSRR61 pKa = 11.84TPLRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84AIVRR71 pKa = 11.84RR72 pKa = 11.84SPPRR76 pKa = 11.84SRR78 pKa = 11.84RR79 pKa = 3.46

Molecular weight: 9.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4452

0

4452

1471220

30

3545

330.5

35.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.679 ± 0.052

0.79 ± 0.01

6.881 ± 0.037

5.607 ± 0.042

2.931 ± 0.02

9.33 ± 0.051

2.116 ± 0.015

3.965 ± 0.025

2.314 ± 0.027

9.767 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.81 ± 0.016

2.089 ± 0.028

5.522 ± 0.031

2.784 ± 0.017

7.02 ± 0.047

5.397 ± 0.032

6.321 ± 0.049

9.041 ± 0.037

1.495 ± 0.016

2.143 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski